{"title":"反复暴露于噪声后 C57BL/6j 小鼠耳蜗突触的定量和 mRNA 表达水平。","authors":"Minfei Qian, Zhuowei Yao, Qixuan Wang, Yaqi Zhou, Zhiwu Huang, Jiping Li","doi":"10.1080/00016489.2024.2413385","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Noise-induced cochlear synaptopathy has recently emerged as a focus in hearing research.</p><p><strong>Purpose: </strong>This study aimed to examine the impact of repeated noise exposure on the quantification and mRNA expression levels of cochlear synapses.</p><p><strong>Methods: </strong>Measurements were conducted at baseline, 1 day, and 14 days post-exposure to 88 or 97 dB SPL noise (2 h/day for 7 days, frequency range 2-20 kHz). Auditory brainstem responses (ABRs), immunofluorescence and quantitative real-time PCR (qRT-PCR) were used to examine the results.</p><p><strong>Results: </strong>1. Exposure to 88 dB SPL caused minimal changes in ABRs, ribbon morphology and medial olivocochlear (MOC) efferent synapses; elevation of synaptophysin(SYP) and α9α10 nAchR mRNA levels were observed. 2. Exposure to 97 dB SPL caused threshold shift and synaptopathy of ribbon and MOC; downregulation of α10nAchR, SYP and ctbp2 mRNA levels were observed.</p><p><strong>Conclusion: </strong>Noise-induced cochlear synaptic degeneration involves both afferent and efferent synaptopathy.</p>","PeriodicalId":6880,"journal":{"name":"Acta Oto-Laryngologica","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The quantification and mRNA expression levels of cochlear synapses in C57BL/6j mice following repeated exposure to noise.\",\"authors\":\"Minfei Qian, Zhuowei Yao, Qixuan Wang, Yaqi Zhou, Zhiwu Huang, Jiping Li\",\"doi\":\"10.1080/00016489.2024.2413385\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Noise-induced cochlear synaptopathy has recently emerged as a focus in hearing research.</p><p><strong>Purpose: </strong>This study aimed to examine the impact of repeated noise exposure on the quantification and mRNA expression levels of cochlear synapses.</p><p><strong>Methods: </strong>Measurements were conducted at baseline, 1 day, and 14 days post-exposure to 88 or 97 dB SPL noise (2 h/day for 7 days, frequency range 2-20 kHz). Auditory brainstem responses (ABRs), immunofluorescence and quantitative real-time PCR (qRT-PCR) were used to examine the results.</p><p><strong>Results: </strong>1. Exposure to 88 dB SPL caused minimal changes in ABRs, ribbon morphology and medial olivocochlear (MOC) efferent synapses; elevation of synaptophysin(SYP) and α9α10 nAchR mRNA levels were observed. 2. Exposure to 97 dB SPL caused threshold shift and synaptopathy of ribbon and MOC; downregulation of α10nAchR, SYP and ctbp2 mRNA levels were observed.</p><p><strong>Conclusion: </strong>Noise-induced cochlear synaptic degeneration involves both afferent and efferent synaptopathy.</p>\",\"PeriodicalId\":6880,\"journal\":{\"name\":\"Acta Oto-Laryngologica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Oto-Laryngologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/00016489.2024.2413385\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"OTORHINOLARYNGOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Oto-Laryngologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/00016489.2024.2413385","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
The quantification and mRNA expression levels of cochlear synapses in C57BL/6j mice following repeated exposure to noise.
Background: Noise-induced cochlear synaptopathy has recently emerged as a focus in hearing research.
Purpose: This study aimed to examine the impact of repeated noise exposure on the quantification and mRNA expression levels of cochlear synapses.
Methods: Measurements were conducted at baseline, 1 day, and 14 days post-exposure to 88 or 97 dB SPL noise (2 h/day for 7 days, frequency range 2-20 kHz). Auditory brainstem responses (ABRs), immunofluorescence and quantitative real-time PCR (qRT-PCR) were used to examine the results.
Results: 1. Exposure to 88 dB SPL caused minimal changes in ABRs, ribbon morphology and medial olivocochlear (MOC) efferent synapses; elevation of synaptophysin(SYP) and α9α10 nAchR mRNA levels were observed. 2. Exposure to 97 dB SPL caused threshold shift and synaptopathy of ribbon and MOC; downregulation of α10nAchR, SYP and ctbp2 mRNA levels were observed.
Conclusion: Noise-induced cochlear synaptic degeneration involves both afferent and efferent synaptopathy.
期刊介绍：
Acta Oto-Laryngologica is a truly international journal for translational otolaryngology and head- and neck surgery. The journal presents cutting-edge papers on clinical practice, clinical research and basic sciences. Acta also bridges the gap between clinical and basic research.
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