将唾液检测作为自然感染患者霍乱弧菌监测工具的诊断准确性。

IF 3.4 3区 医学 Q3 IMMUNOLOGY Clinical and experimental immunology Pub Date : 2024-10-21 DOI:10.1093/cei/uxae092
Caroline C Chisenga, Bernard Phiri, Harriet Ng'ombe, Mutinta Muchimba, Fraser Liswaniso, Biana Bernshtein, Adam F Cunningham, David Sack, Samuel Bosomprah
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引用次数: 0

摘要

唾液作为一种诊断介质,因其非侵入性的采集方式而有望成为血液的替代品,从而提高患者的依从性,尤其是在儿科和老年病人群中。在这项研究中,我们评估了唾液作为非侵入性介质测量自然感染者体内霍乱弧菌特异性血清抗体的实用性。我们对参加一项队列研究的 63 名霍乱患者的血清和唾液样本进行了配对检测。以杀菌抗体测定法(IgM/IgG)为标记,准确测定霍乱特异性抗体水平。利用接收器操作特征曲线(ROC),我们发现使(灵敏度+特异性)最大化的最佳临界值是 10 滴度。在此唾液滴度下,灵敏度为 76.9%(95%CI:60.9%,87.7%),特异性为 80.0%(95%CI:56.6%,92.5%)。使用斯皮尔曼相关系数(Spearman's correlation coefficient),我们还发现霍乱弧菌唾液抗体与血清抗体之间存在正相关(rho=0.66,p<0.05)。
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Diagnostic Accuracy of Saliva-based Testing as a Vibrio Cholerae Surveillance Tool among naturally-infected patients.

Saliva, as a diagnostic medium, offers a promising alternative to blood by virtue of its non-invasive collection, which enhances patient compliance, especially in paediatric and geriatric populations. In this study, we assessed the utility of saliva as a non-invasive medium for measuring V. cholerae-specific serum antibodies in naturally infected individuals. We tested paired serum and saliva samples obtained from a total of 63 cholera patients enrolled in a cohort study. Vibriocidal antibodies assay (IgM/IgG) as markers for accurate determination was used to determine cholera specific antibody levels. Using receiver operating characteristics (ROC) curve, we found that the best cut-off that maximizes (sensitivity + specificity) is 10 titres. At this saliva titre, the sensitivity is 76.9% (95%CI: 60.9%, 87.7%) and specificity is 80.0% (95%CI: 56.6%, 92.5%). Using Spearman's correlation coefficient, we also found evidence of a positive correlation between Vibrio Cholerae saliva and serum antibodies (rho=0.66, p<0.001). In conclusion, saliva-based diagnostic cholera tests has high diagnostic accuracy, and would be advantageous, cheaper, and quicker for early diagnosis of severe cholera outcomes.

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来源期刊
CiteScore
8.40
自引率
2.20%
发文量
101
审稿时长
3-8 weeks
期刊介绍: Clinical & Experimental Immunology (established in 1966) is an authoritative international journal publishing high-quality research studies in translational and clinical immunology that have the potential to transform our understanding of the immunopathology of human disease and/or change clinical practice. The journal is focused on translational and clinical immunology and is among the foremost journals in this field, attracting high-quality papers from across the world. Translation is viewed as a process of applying ideas, insights and discoveries generated through scientific studies to the treatment, prevention or diagnosis of human disease. Clinical immunology has evolved as a field to encompass the application of state-of-the-art technologies such as next-generation sequencing, metagenomics and high-dimensional phenotyping to understand mechanisms that govern the outcomes of clinical trials.
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