通过发光酚化学发光法估算人类骸骨的死后间隔。

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL International Journal of Legal Medicine Pub Date : 2024-10-18 DOI:10.1007/s00414-024-03343-8
Catarina Ermida, Joana Rosa, Eugénia Cunha, Maria Teresa Ferreira
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引用次数: 0

摘要

死后间隔(PMI)估算是法医学中的一项重大挑战,尤其是在处理人类遗骸时。用于区分法医感兴趣的可能遗骸的筛选方案是司法目的的重要工具。在这种情况下,发光酚化学发光法是一种很有前途的方法,总体成本低,所需时间短。这种方法主要用作推定检验,因为化学发光反应的强度会随着死后间隔时间的延长而降低,从而强调了其实际意义。本研究旨在扩展之前关于鲁米诺化学发光潜力的研究,评估其在估计死后间隔时间方面的实用性。我们的样本包括 239 个已知 PMI 的人体锁骨。在暗室中将发光酚溶液喷洒在每个骨粉样本上,用肉眼观察并拍照。本研究结果表明,该方法是一种适用于推测 PMI 的工具,可减少刑事调查的时间和成本。研究结果强调了发光酚化学发光法检测近期 PMI 的高灵敏度,但也突出了假阳性的显著发生率。因此,我们的研究结果证实鲁米诺化学发光法是确定死亡时间的有力工具,特别是在鉴定法医相关遗骸时。不过,相对较低的特异性表明,该方法应辅以其他测试,以进一步确认和科学验证遗骸的法医相关性。
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Postmortem interval estimation of human skeletonized remains through luminol chemiluminescence.

Postmortem interval (PMI) estimation represents a significant challenge in the forensic sciences, particularly when dealing with human skeletal remains. A screening protocol for distinguishing possible remains of forensic interest is a crucial tool for judicial purposes. In this context, luminol chemiluminescence emerges as a promising method, with low overall costs and required time. This method is primarily used as a presumptive test, based on the understanding that the intensity of the chemiluminescence reactions decreases with an increase in the postmortem interval, thus underlining its practical implications.This research aims to expand previous research on the potential of luminol chemiluminescence, evaluating its usefulness in estimating PMI. Our sample comprised 239 human clavicles, with known PMI. The luminol solution was sprayed on each powder bone sample in a dark room, observed by the naked eye and photographed. The intensity of the chemiluminescence reaction was measured using a binary and a 5-level scale.The present results reveal that this method is a suitable tool for PMI estimation as a presumptive test, reducing time and costs in criminal investigations. The findings underscore the high sensitivity of luminol chemiluminescence for detecting recent PMI but also highlight a notable incidence of false positives. Thus, our results confirm luminol chemiluminescence as a powerful tool for dating time of death, particularly for identifying forensic relevant remains. Still, the relatively low specificity indicates that it should be complemented with additional tests for further confirmation and scientific validation of the remains' forensic relevance.

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来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
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