{"title":"BTB基因超家族的全基因组鉴定和表达分析为了解中华短吻鳄的性别决定和早期性腺发育提供了线索","authors":"Pengfei Li, Peng Liu, Dongsheng Zang, Changcheng Li, Chong Wang, Yunzhen Zhu, Mengqin Liu, Lilei Lu, Xiaobing Wu, Haitao Nie","doi":"10.3390/ijms251910771","DOIUrl":null,"url":null,"abstract":"<p><p>The <i>BTB</i> gene superfamily is widely distributed among higher eukaryotes and plays a significant role in numerous biological processes. However, there is limited knowledge about the structure and function of <i>BTB</i> genes in the critically endangered species <i>Alligator sinensis</i>, which is endemic to China. A total of 170 <i>BTB</i> genes were identified from the <i>A. sinensis</i> genome, classified into 13 families, and unevenly distributed across 16 chromosomes. Analysis of gene duplication events yielded eight pairs of tandem duplication genes and six pairs of segmental duplication genes. Phylogenetics shows that the <i>AsBTB</i> genes are evolutionarily conserved. The cis-regulatory elements in the <i>AsBTB</i> family promoter region reveal their involvement in multiple biological processes. Protein interaction network analysis indicates that the protein interactions of the <i>AsBTB</i> genes are centered around CLU-3, mainly participating in the regulation of biological processes through the ubiquitination pathway. The expression profile and protein interaction network analysis of <i>AsBTB</i> genes during sex differentiation and early gonadal development indicate that <i>AsBTB</i> genes are widely expressed in this process and involves numerous genes and pathways for regulation. This study provides a basis for further investigation of the role of the <i>BTB</i> gene in sex differentiation and gonadal development in <i>A. sinensis</i>.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11477308/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genome-Wide Identification and Expression Analysis of the <i>BTB</i> Gene Superfamily Provides Insight into Sex Determination and Early Gonadal Development of <i>Alligator sinensis</i>.\",\"authors\":\"Pengfei Li, Peng Liu, Dongsheng Zang, Changcheng Li, Chong Wang, Yunzhen Zhu, Mengqin Liu, Lilei Lu, Xiaobing Wu, Haitao Nie\",\"doi\":\"10.3390/ijms251910771\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The <i>BTB</i> gene superfamily is widely distributed among higher eukaryotes and plays a significant role in numerous biological processes. However, there is limited knowledge about the structure and function of <i>BTB</i> genes in the critically endangered species <i>Alligator sinensis</i>, which is endemic to China. A total of 170 <i>BTB</i> genes were identified from the <i>A. sinensis</i> genome, classified into 13 families, and unevenly distributed across 16 chromosomes. Analysis of gene duplication events yielded eight pairs of tandem duplication genes and six pairs of segmental duplication genes. Phylogenetics shows that the <i>AsBTB</i> genes are evolutionarily conserved. The cis-regulatory elements in the <i>AsBTB</i> family promoter region reveal their involvement in multiple biological processes. Protein interaction network analysis indicates that the protein interactions of the <i>AsBTB</i> genes are centered around CLU-3, mainly participating in the regulation of biological processes through the ubiquitination pathway. The expression profile and protein interaction network analysis of <i>AsBTB</i> genes during sex differentiation and early gonadal development indicate that <i>AsBTB</i> genes are widely expressed in this process and involves numerous genes and pathways for regulation. This study provides a basis for further investigation of the role of the <i>BTB</i> gene in sex differentiation and gonadal development in <i>A. sinensis</i>.</p>\",\"PeriodicalId\":14156,\"journal\":{\"name\":\"International Journal of Molecular Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11477308/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Molecular Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/ijms251910771\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Molecular Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/ijms251910771","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genome-Wide Identification and Expression Analysis of the BTB Gene Superfamily Provides Insight into Sex Determination and Early Gonadal Development of Alligator sinensis.
The BTB gene superfamily is widely distributed among higher eukaryotes and plays a significant role in numerous biological processes. However, there is limited knowledge about the structure and function of BTB genes in the critically endangered species Alligator sinensis, which is endemic to China. A total of 170 BTB genes were identified from the A. sinensis genome, classified into 13 families, and unevenly distributed across 16 chromosomes. Analysis of gene duplication events yielded eight pairs of tandem duplication genes and six pairs of segmental duplication genes. Phylogenetics shows that the AsBTB genes are evolutionarily conserved. The cis-regulatory elements in the AsBTB family promoter region reveal their involvement in multiple biological processes. Protein interaction network analysis indicates that the protein interactions of the AsBTB genes are centered around CLU-3, mainly participating in the regulation of biological processes through the ubiquitination pathway. The expression profile and protein interaction network analysis of AsBTB genes during sex differentiation and early gonadal development indicate that AsBTB genes are widely expressed in this process and involves numerous genes and pathways for regulation. This study provides a basis for further investigation of the role of the BTB gene in sex differentiation and gonadal development in A. sinensis.
期刊介绍:
The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).