膳食摄入根瘤菌生物质的亚慢性毒性评估

IF 2.7 4区 医学 Q3 TOXICOLOGY Journal of Applied Toxicology Pub Date : 2024-10-20 DOI:10.1002/jat.4713
Kevin Scaife, Kirt R Phipps, Daniella Scalise, Olivér Polgár
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引用次数: 0

摘要

真菌衍生食品符合可持续食品供应原则,为消费者提供了一种可持续的营养选择。根瘤菌(Rhizomucor pusillus)菌株 CBS 143028 已成为一种候选食品成分。R. pusillus CBS 143028 发酵后产生的菌丝生物质主要包括真菌蛋白质、细胞壁成分和微量营养素。虽然乳杆菌在食品酶的生产和传统发酵食品中已有安全使用的历史,但乳杆菌 CBS 143028 发酵后获得的真菌生物质被认为是一种新型食品,需要进行全面的安全性评估。为此,我们对 Wistar 大鼠(10 只/性别/组)进行了为期 90 天的口服毒性研究,给它们喂食的食物中分别含有 0、100,000、200,000 或 300,000 ppm 的 R. pusillus 菌丝体。对 OECD 试验指南 408 中给出的标准毒性研究参数进行了检测。在低剂量组、中剂量组和高剂量组中,R. pusillus 菌丝体的平均剂量分别为男性 6398、12738 或 19668 毫克/千克体重/天,女性 7235、14949 或 22461 毫克/千克体重/天。尽管报告的差异具有统计学意义,但由于对照组的数值不典型,缺乏剂量反应关系,或归因于正常的生物变化,这些影响被认为不具有生物相关性或与试验品无关。因此,无观测不良效应水平(NOAEL)被确定为 300,000 ppm(男性和女性分别相当于 19,668 毫克/千克体重/天和 22,461 毫克/千克体重/天),这是测试的最高浓度。
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Subchronic Toxicity Evaluation of Dietary Administration of a Fungal Biomass From Rhizomucor pusillus.

Fungal-derived food products align with sustainable food supply principles and offer a sustainable and nutritious option for consumers. Rhizomucor pusillus strain CBS 143028 has emerged as a candidate food ingredient. Fermentation of R. pusillus CBS 143028 results in a mycelium biomass mainly comprising fungal proteins, cell wall components, and micronutrients. Although R. pusillus has a history of safe use in the production of food enzymes and in traditional fermented foods, the fungal biomass obtained after fermentation of R. pusillus CBS 143028 is considered a novel food and requires a thorough safety assessment. To this end, a 90-day oral toxicity study was conducted in which Wistar rats (10/sex/group) were provided diets containing 0, 100,000, 200,000, or 300,000 ppm of R. pusillus mycelium. Standard toxicity study parameters as given in OECD Test Guideline 408 were examined. The mean achieved dosages of R. pusillus mycelium were 6398, 12,738, or 19,668 mg/kg body weight/day for males and 7235, 14,949, or 22,461 mg/kg body weight/day for females in the low-, mid-, and high-dose groups, respectively. Although statistically significant differences were reported, these effects were not considered biologically relevant or test article-related due to atypical values in the control groups, the lack of a dose response relationship, or were attributed to normal biological variation. Thus, the no-observed-adverse-effect level (NOAEL) was established at 300,000 ppm (corresponding to 19,668 and 22,461 mg/kg body weight/day for males and females, respectively), the highest concentration tested.

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来源期刊
CiteScore
7.00
自引率
6.10%
发文量
145
审稿时长
1 months
期刊介绍: Journal of Applied Toxicology publishes peer-reviewed original reviews and hypothesis-driven research articles on mechanistic, fundamental and applied research relating to the toxicity of drugs and chemicals at the molecular, cellular, tissue, target organ and whole body level in vivo (by all relevant routes of exposure) and in vitro / ex vivo. All aspects of toxicology are covered (including but not limited to nanotoxicology, genomics and proteomics, teratogenesis, carcinogenesis, mutagenesis, reproductive and endocrine toxicology, toxicopathology, target organ toxicity, systems toxicity (eg immunotoxicity), neurobehavioral toxicology, mechanistic studies, biochemical and molecular toxicology, novel biomarkers, pharmacokinetics/PBPK, risk assessment and environmental health studies) and emphasis is given to papers of clear application to human health, and/or advance mechanistic understanding and/or provide significant contributions and impact to their field.
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