中国一个携带 mcr-8 的 ST65 型高病毒性肺炎克雷伯氏菌分离株的基因组序列。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-10-19 DOI:10.1016/j.jgar.2024.09.006
Weili Zhang, Hao Xu, Yaling Li, Ruishan Liu, Danfeng Lou
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引用次数: 0

摘要

研究目的本研究报告了来自中国的携带 mcr-8 的 ST65 高病毒性肺炎克雷伯菌分离株的完整基因组序列。目的是研究其分子特征和耐药机制:方法:从中国的一名住院病人身上分离出耐药秋水仙碱的 hvKP。全基因组测序在 Illumina NovaSeq 6000 和长线程 ONT 平台上进行。进行了 S1 核酸酶脉冲场凝胶电泳、Southern-blot 和抗菌药物敏感性检测。根据序列预测了质粒的序列类型、抗菌性和毒力相关基因。通过 BLAST 环形图像绘制了多质粒比较的环形图:结果:肺炎双球菌 ACESH00926 的完整基因组序列由一条染色体和两条质粒组成。根据 MLST 方案,ACESH00926 属于 K2 ST65。ACESH00926 对可乐定表现出高度耐药性(MIC > 8 µg/mL)。发现了几个ARGs,包括位于IncFIl(K)/IncFIA(HI1)型质粒中的可移动的耐秋水仙素基因mcr-8。更大的质粒是一个类似 pK244 的毒力质粒。它携带了一系列毒力基因,如粘液表型调节基因(rmpA 和 rmpA2)、盐霉素苷元生物合成基因(iroB)、ABC 转运体基因(iroC)、铁锈气动杆菌素受体基因(iutA)、气动杆菌素苷元生物合成蛋白基因(iucC)和气动杆菌素合成酶基因(iucA)。另一个质粒携带具有保守遗传背景的 mcr-8(dgkA-sasA-copR-mcr-8-ccdA-ccdB-xerD-repE-parM-umuC-lexA-klcA):结论:有必要强调今后监测耐秋水仙碱菌株和高病毒性肺炎克雷伯菌株组合的必要性。
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Genome sequence of a ST65 hypervirulent Klebsiella pneumoniae isolate carrying mcr-8 from China.

Objectives: In this study, we report the complete genome sequence of a ST65 hypervirulent Klebsiella pneumoniae isolate carrying mcr-8 from China. The aim was to investigate its molecular characteristics and resistant mechanism.

Methods: A colistin-resistant hvKP was isolated from an inpatient in China. The whole genome was sequenced on Illumina NovaSeq 6000 and long-read ONT platforms. de novo assembly was conducted using SPAdes and Unicycler. S1 nuclease pulsed-field gel electrophoresis, Southern-blot, and antimicrobial susceptibility testing were performed. Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. The circular maps of multiple plasmids comparisons were drawn by the BLAST Ring Image.

Results: The complete genome sequence of K. pneumoniae ACESH00926 consists of one chromosome and two plasmids. ACESH00926 belongs to K2 ST65 according to the MLST scheme. ACESH00926 showed high resistance to colistin (MIC > 8 µg/mL). Several ARGs were identified, including mobile colistin-resistant gene mcr-8 which was located in an IncFIl(K)/IncFIA(HI1) type plasmid. The bigger plasmid was a pK244-like virulence plasmid. It carrying a series of virulence genes, such as the regulator of the mucoid phenotype (rmpA and rmpA2), salmochelin siderophore biosynthesis (iroB), ABC transporter (iroC), ferric aerobactin receptor (iutA), aerobactin siderophore biosynthesis protein (iucC), and aerobactin synthetase (iucA) encoded genes. And another plasmid carrying mcr-8 with a conserved genetic context (dgkA-sasA-copR-mcr-8-ccdA-ccdB-xerD-repE-parM-umuC-lexA-klcA).

Conclusions: It is necessary to emphasize the necessity of monitoring a combination of colistin-resistant and hypervirulent Klebsiella pneumoniae strains in the future.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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