从边缘到主流:ETD MS 如何让大众认识 O-GlcNAc。

IF 6.1 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS Molecular & Cellular Proteomics Pub Date : 2024-10-15 DOI:10.1016/j.mcpro.2024.100859
Namrata D Udeshi, Gerald W Hart, Chad Slawson
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引用次数: 0

摘要

O-GlcNAcylation 于 20 世纪 80 年代由 Torres 和 Hart 发现(1),可修饰数千种细胞蛋白质,但与磷酸化等其他循环翻译后修饰的大量机理信息相比,人们对 O-GlcNAc 的调控作用仍然知之甚少。研究 O-GlcNAcylation 面临许多挑战,其中包括质谱分析的技术障碍。多年来,许多研究小组开发出了研究 O-GlcNAcylation 的重要方法,揭示了它在细胞中的作用。本视角旨在回顾围绕 O-GlcNAc 研究的挑战和创新,并记录 Donald F. Hunt 及其实验室的工作,特别是开发 ETD 及其在该研究领域的应用。
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From Fringe to the Mainstream: How ETD MS Brought O-GlcNAc to the Masses.

O-GlcNAcylation was identified in the 1980s by Torres and Hart and modifies thousands of cellular proteins, yet the regulatory role of O-GlcNAc is still poorly understood compared to the abundance of mechanistic information known for other cycling post-translational modifications like phosphorylation. Many challenges are associated with studying O-GlcNAcylation and are tied to the technical hurdles with analysis by mass spectrometry. Over the years, many research groups have developed important methods to study O-GlcNAcylation revealing its role in the cell, and this perspective aims to review the challenges and innovations around O-GlcNAc research and chronicle the work by Donald F. Hunt and his laboratory, particularly in development of ETD and its application to this field of research.

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来源期刊
Molecular & Cellular Proteomics
Molecular & Cellular Proteomics 生物-生化研究方法
CiteScore
11.50
自引率
4.30%
发文量
131
审稿时长
84 days
期刊介绍: The mission of MCP is to foster the development and applications of proteomics in both basic and translational research. MCP will publish manuscripts that report significant new biological or clinical discoveries underpinned by proteomic observations across all kingdoms of life. Manuscripts must define the biological roles played by the proteins investigated or their mechanisms of action. The journal also emphasizes articles that describe innovative new computational methods and technological advancements that will enable future discoveries. Manuscripts describing such approaches do not have to include a solution to a biological problem, but must demonstrate that the technology works as described, is reproducible and is appropriate to uncover yet unknown protein/proteome function or properties using relevant model systems or publicly available data. Scope: -Fundamental studies in biology, including integrative "omics" studies, that provide mechanistic insights -Novel experimental and computational technologies -Proteogenomic data integration and analysis that enable greater understanding of physiology and disease processes -Pathway and network analyses of signaling that focus on the roles of post-translational modifications -Studies of proteome dynamics and quality controls, and their roles in disease -Studies of evolutionary processes effecting proteome dynamics, quality and regulation -Chemical proteomics, including mechanisms of drug action -Proteomics of the immune system and antigen presentation/recognition -Microbiome proteomics, host-microbe and host-pathogen interactions, and their roles in health and disease -Clinical and translational studies of human diseases -Metabolomics to understand functional connections between genes, proteins and phenotypes
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