基于蛋白质组分析的紫锥菊苷防治缺氧性肺动脉高压的机制研究

IF 2.9 4区 医学 Q2 PHARMACOLOGY & PHARMACY Pharmacology Research & Perspectives Pub Date : 2024-10-01 DOI:10.1002/prp2.70025
Xiangyun Gai, Qingqing Xia, Hongmai Wang, Hongtao Bi, Jinyu Wang, Yuefu Zhao
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引用次数: 0

摘要

缺氧性肺动脉高压(HPH)是一种影响心肺系统的慢性疾病,死亡率很高。棘白甙是一种苯乙醇苷,可用于改善大鼠肺血管重塑和肺血管收缩。因此,我们旨在探索ECH预防和治疗HPH的机制。将 Sprague Dawley 大鼠饲养在低压缺氧箱中 28 天,以获得 HPH 模型。实验大鼠被随机分为以下几组:常氧组、慢性缺氧组和 ECH 组。ECH(10、20和40 mg/kg)的治疗结果显示,ECH可降低HPH大鼠的mPAP、Hb、Hct和RVHI。随后,本研究采用无标记定量蛋白质组分析、Western印迹和RT-PCR等方法研究了ECH预防HPH的机制。结果发现,在慢性缺氧组中,ACSL1、COL6A1、COL4A2、COL1A1和PC的水平比正常缺氧组升高。然而,在接受 ECH 治疗的慢性缺氧组中却观察到了相反的效果。该研究表明,服用 ECH 可抑制炎症反应、抑制平滑肌细胞增殖并减少细胞外基质的沉积,从而减缓 HPH 的病理进展。
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Study on the mechanism of echinacoside in preventing and treating hypoxic pulmonary hypertension based on proteomic analyses.

Hypoxic pulmonary hypertension (HPH), a chronic condition affecting the cardiopulmonary system, has high mortality. Echinacoside (ECH) is a phenylethanoid glycoside, which is used to ameliorate pulmonary vascular remodeling and pulmonary vasoconstriction in rats. Accordingly, we aimed to explore the mechanism of ECH in preventing and treating HPH. Sprague Dawley rats were housed in a hypobaric hypoxia chamber for 28 days to obtain the HPH model. The experimental rats were randomly allocated into the following several groups: normoxia group, chronic hypoxia group, and ECH group. The therapeutic results of ECH (10, 20, and 40 mg/kg) showed that ECH reduced mPAP, Hb, Hct, and RVHI in HPH rats. Then this work employed label-free quantitative proteomic analysis, western blotting, and RT-PCR to investigate the mechanism by which ECH prevents HPH. The results found that in the chronic hypoxia group, the levels of ACSL1, COL6A1, COL4A2, COL1A1, and PC increased compared to the normoxia group. However, the opposite effect was observed in the chronic hypoxia group treated with ECH. The study indicates that the administration of ECH may slow the pathological progression of HPH by suppressing the inflammatory response, inhibiting smooth muscle cell proliferation, and minimizing the deposition of extracellular matrix.

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来源期刊
Pharmacology Research & Perspectives
Pharmacology Research & Perspectives Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
5.30
自引率
3.80%
发文量
120
审稿时长
20 weeks
期刊介绍: PR&P is jointly published by the American Society for Pharmacology and Experimental Therapeutics (ASPET), the British Pharmacological Society (BPS), and Wiley. PR&P is a bi-monthly open access journal that publishes a range of article types, including: target validation (preclinical papers that show a hypothesis is incorrect or papers on drugs that have failed in early clinical development); drug discovery reviews (strategy, hypotheses, and data resulting in a successful therapeutic drug); frontiers in translational medicine (drug and target validation for an unmet therapeutic need); pharmacological hypotheses (reviews that are oriented to inform a novel hypothesis); and replication studies (work that refutes key findings [failed replication] and work that validates key findings). PR&P publishes papers submitted directly to the journal and those referred from the journals of ASPET and the BPS
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