吸湿性生物活性光固化复合材料可促进牙桥形成。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2024-09-26 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae114
Yunzi Long, Guibin Huang, Siyi Liu, Liju Xu, Ailing Li, Dong Qiu, Yanmei Dong
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引用次数: 0

摘要

一种名为 TheraCal LC 的光固化生物活性复合材料易于操作且固化速度快。但在应用于直接盖髓(DPC)时,吸水性差限制了其生物活性。提高树脂基生物活性材料的吸水性可能是优化光固化生物活性材料生物矿化过程的关键。在这项研究中,我们构建了一种由生物活性玻璃(BG)、聚乙二醇(PEG)和树脂组成的吸湿性光固化生物活性复合材料。生物活性玻璃被封装在多孔剂(即 PEG)中,并与树脂基质混合。电感耦合等离子体显示,与 TheraCal LC 相比,光固化 BG(LC-BG)的离子释放速度更快,离子交换量更大。扫描电子显微镜观察到 BG 微粒上形成了大孔和羟基磷灰石晶体涂层。树脂和 LC-BG 组之间的剪切粘接强度与 TheraCal LC 组没有显著差异。CCK-8 检测表明,LC-BG 提取物无毒。实时聚合酶链反应显示,LC-BG 能上调人牙髓细胞中牙源性基因的表达。DPC 检测证明,LC-BG 组在牙本质小管形成(P = 0.659)或牙本质母细胞样细胞层形成(P = 0.155)方面与 TheraCal LC 组无显著差异,但钙化桥的完整性明显优于 TheraCal LC 组(P = 0.039);与 TheraCal LC 组相比,LC-BG 组检测到更多的 DSPP 阳性和 DMP-1 阳性细胞。虽然 LC-BG 组和 TheraCal LC 组在牙髓炎症细胞浸润方面无明显差异(P = 0.476),但 LC-BG 组检测到的白细胞介素 1β 阳性和肿瘤坏死因子 α 阳性细胞少于 TheraCal LC 组。总之,新开发的吸湿性 LC-BG 复合材料在体外比 TheraCal LC 表现出更好的生物活性和牙本质分化,在体内比 TheraCal LC 诱导出更好的钙化桥完整性。
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Hygroscopic bioactive light-cured composite promoting dentine bridge formation.

A light-cured bioactive composite, TheraCal LC, is easy to handle and fast-setting. But poor water absorption restricted its bioactivity when applied in direct pulp capping (DPC). Enhancing the water absorption of resin-based bioactive materials may be key to optimizing biomineralization procedure of light-cured bioactive materials. We constructed a hygroscopic, light-cured bioactive composite made up of bioactive glass (BG), poly (ethylene glycol) (PEG) and resin in this study. BG was encapsulated into a porogen (i.e. PEG) and mixed into resin matrix. Inductively coupled plasma showed that light-cured BG (LC-BG) exhibited faster ion release and more ion exchange than TheraCal LC did. The formation of macropores and hydroxyapatite crystal coatings on the BG microparticles was observed using scanning electron microscopy. The shear bond strength between the resin and LC-BG group did not significantly differ from the TheraCal LC group. CCK-8 assay showed that the LC-BG extract was nontoxic. Real-time polymerase chain reaction revealed that LC-BG upregulated odontogenic gene expression in human dental pulp cells. DPC assay proved that the LC-BG group exhibited no significant difference in dentin tubule formation (P = 0.659) or odontoblast-like cell layer formation (P = 0.155) from the TheraCal LC group, but exhibited significantly better integrity of the calcified bridge than the TheraCal LC group (P =0.039); more DSPP-positive and DMP-1-positive cells were detected in the LC-BG group than in the TheraCal LC group. Although no significant difference in pulpal inflammatory cell infiltration was observed between the LC-BG group and the TheraCal LC group (P = 0.476), fewer interleukin 1β-positive and tumor necrosis factor α-positive cells were detected in the LC-BG group than in the TheraCal LC group. In conclusion, the newly developed hygroscopic LC-BG composite showed better bioactivity and odontogenic differentiation than the TheraCal LC did in vitro and induced better integrity of the calcified bridge than the TheraCal LC did in vivo.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
期刊最新文献
Correction to: Constructing a highly efficient multifunctional carbon quantum dot platform for the treatment of infectious wounds. Non-invasive fast assessment of hepatic injury through computed tomography imaging with renal-clearable Bi-DTPA dimeglumine. Hygroscopic bioactive light-cured composite promoting dentine bridge formation. Biological properties and characterization of several variations of a clinical human plasma-based skin substitute model and its manufacturing process. The effects of heat and hydrogen peroxide treatment on the osteoinductivity of demineralized cortical bone: a potential method for preparing tendon/ligament repair scaffolds.
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