Kwan-Woo Lee, Su-Hyun Mun, Yeon-A Kim, Hyo-Rim Kim, Qinglong Jin, Min-Ki Lee, Soo Nam Park
{"title":"Koenigia alpina 提取物的功能特性和成分。","authors":"Kwan-Woo Lee, Su-Hyun Mun, Yeon-A Kim, Hyo-Rim Kim, Qinglong Jin, Min-Ki Lee, Soo Nam Park","doi":"10.1111/srt.70102","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Koenigia alpina (All.) T.M.Schust. & Reveal (alpine knotweed) is a perennial herb belonging to the Polygonaceae family. Several studies have examined Polygonaceae species' potential applications as cosmeceutical materials; however, the potential of K. alpina as a cosmeceutical has not yet been studied.</p><p><strong>Materials and methods: </strong>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipopolysaccharide were used to induce an inflammatory response in RAW 264.7 cells. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals and H<sub>2</sub>O<sub>2</sub> were used to evaluate the free-radical scavenging activity of K. alpina extract and its protective effect against reactive oxygen species (ROS)-induced cell damage. The whitening, antiaging, and cell proliferation/migration effects of the extracts were evaluated via tyrosinase inhibition, collagenase/elastase inhibition, and wound healing assays, respectively. The anti-inflammatory effect was confirmed by evaluating nitric oxide (NO) production in RAW 264.7 cells. High-performance liquid chromatography (HPLC), UV, and MS/MS were used to determine the main components of the extract and fractions.</p><p><strong>Results: </strong>The ethyl acetate (EA) fraction and its aglycone fraction showed very high free-radical scavenging activities (47.5 and 47.1 µg/mL, respectively). The extract/fractions also showed significant tyrosinase inhibition (IC<sub>50</sub> = 0.38 mg/mL in EA fraction), collagenase inhibition (IC<sub>50</sub> = 0.21 mg/mL in EA fraction), and elastase inhibition (IC<sub>50</sub> = 0.57 mg/mL in aglycone fraction). NO production in lipopolysaccharide-induced RAW 264.7 cells was inhibited by the extract/fractions. The extract also promoted the closure of scratch wounds in HaCaT cells. The K. alpina extract/fractions contained cardamonin, quercetin, and quercitrin.</p><p><strong>Conclusion: </strong>K. alpina extracts/fractions showed antioxidant, antiaging, whitening, and anti-inflammatory activities, suggesting they may have potential as antiaging cosmeceuticals.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"30 10","pages":"e70102"},"PeriodicalIF":2.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465873/pdf/","citationCount":"0","resultStr":"{\"title\":\"Functional Properties and Components of Koenigia alpina Extract.\",\"authors\":\"Kwan-Woo Lee, Su-Hyun Mun, Yeon-A Kim, Hyo-Rim Kim, Qinglong Jin, Min-Ki Lee, Soo Nam Park\",\"doi\":\"10.1111/srt.70102\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Koenigia alpina (All.) T.M.Schust. & Reveal (alpine knotweed) is a perennial herb belonging to the Polygonaceae family. Several studies have examined Polygonaceae species' potential applications as cosmeceutical materials; however, the potential of K. alpina as a cosmeceutical has not yet been studied.</p><p><strong>Materials and methods: </strong>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipopolysaccharide were used to induce an inflammatory response in RAW 264.7 cells. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals and H<sub>2</sub>O<sub>2</sub> were used to evaluate the free-radical scavenging activity of K. alpina extract and its protective effect against reactive oxygen species (ROS)-induced cell damage. The whitening, antiaging, and cell proliferation/migration effects of the extracts were evaluated via tyrosinase inhibition, collagenase/elastase inhibition, and wound healing assays, respectively. The anti-inflammatory effect was confirmed by evaluating nitric oxide (NO) production in RAW 264.7 cells. High-performance liquid chromatography (HPLC), UV, and MS/MS were used to determine the main components of the extract and fractions.</p><p><strong>Results: </strong>The ethyl acetate (EA) fraction and its aglycone fraction showed very high free-radical scavenging activities (47.5 and 47.1 µg/mL, respectively). The extract/fractions also showed significant tyrosinase inhibition (IC<sub>50</sub> = 0.38 mg/mL in EA fraction), collagenase inhibition (IC<sub>50</sub> = 0.21 mg/mL in EA fraction), and elastase inhibition (IC<sub>50</sub> = 0.57 mg/mL in aglycone fraction). NO production in lipopolysaccharide-induced RAW 264.7 cells was inhibited by the extract/fractions. The extract also promoted the closure of scratch wounds in HaCaT cells. The K. alpina extract/fractions contained cardamonin, quercetin, and quercitrin.</p><p><strong>Conclusion: </strong>K. alpina extracts/fractions showed antioxidant, antiaging, whitening, and anti-inflammatory activities, suggesting they may have potential as antiaging cosmeceuticals.</p>\",\"PeriodicalId\":21746,\"journal\":{\"name\":\"Skin Research and Technology\",\"volume\":\"30 10\",\"pages\":\"e70102\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465873/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Skin Research and Technology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/srt.70102\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Skin Research and Technology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/srt.70102","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景介绍Koenigia alpina (All.) T.M.Schust. & Reveal（高山结缕草）属于蓼科多年生草本植物。材料与方法：使用过氧化氢（H2O2）和脂多糖诱导 RAW 264.7 细胞产生炎症反应。用 2,2-二苯基-1-苦基肼（DPPH）自由基和 H2O2 评估阿尔卑斯山红景天提取物的自由基清除活性及其对活性氧（ROS）诱导的细胞损伤的保护作用。通过酪氨酸酶抑制、胶原酶/弹性蛋白酶抑制和伤口愈合试验，分别评估了萃取物的美白、抗衰老和细胞增殖/迁移效果。通过评估 RAW 264.7 细胞中一氧化氮（NO）的产生，证实了提取物的抗炎作用。采用高效液相色谱法（HPLC）、紫外光谱法（UV）和质谱/质谱法（MS/MS）测定提取物和馏分的主要成分：结果：乙酸乙酯（EA）馏分及其苷元馏分显示出极高的自由基清除活性（分别为 47.5 和 47.1 µg/mL）。提取物/馏分还显示出显著的酪氨酸酶抑制作用（EA馏分的IC50 = 0.38 mg/mL）、胶原酶抑制作用（EA馏分的IC50 = 0.21 mg/mL）和弹性蛋白酶抑制作用（苷元馏分的IC50 = 0.57 mg/mL）。提取物/馏分可抑制脂多糖诱导的 RAW 264.7 细胞中 NO 的产生。提取物还能促进 HaCaT 细胞划痕伤口的闭合。K. alpina 提取物/萃取物含有豆蔻苷、槲皮素和槲皮苷：K.alpina萃取物/馏分具有抗氧化、抗衰老、美白和抗炎活性，这表明它们可能具有作为抗衰老药妆品的潜力。
Functional Properties and Components of Koenigia alpina Extract.
Background: Koenigia alpina (All.) T.M.Schust. & Reveal (alpine knotweed) is a perennial herb belonging to the Polygonaceae family. Several studies have examined Polygonaceae species' potential applications as cosmeceutical materials; however, the potential of K. alpina as a cosmeceutical has not yet been studied.
Materials and methods: Hydrogen peroxide (H2O2) and lipopolysaccharide were used to induce an inflammatory response in RAW 264.7 cells. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals and H2O2 were used to evaluate the free-radical scavenging activity of K. alpina extract and its protective effect against reactive oxygen species (ROS)-induced cell damage. The whitening, antiaging, and cell proliferation/migration effects of the extracts were evaluated via tyrosinase inhibition, collagenase/elastase inhibition, and wound healing assays, respectively. The anti-inflammatory effect was confirmed by evaluating nitric oxide (NO) production in RAW 264.7 cells. High-performance liquid chromatography (HPLC), UV, and MS/MS were used to determine the main components of the extract and fractions.
Results: The ethyl acetate (EA) fraction and its aglycone fraction showed very high free-radical scavenging activities (47.5 and 47.1 µg/mL, respectively). The extract/fractions also showed significant tyrosinase inhibition (IC50 = 0.38 mg/mL in EA fraction), collagenase inhibition (IC50 = 0.21 mg/mL in EA fraction), and elastase inhibition (IC50 = 0.57 mg/mL in aglycone fraction). NO production in lipopolysaccharide-induced RAW 264.7 cells was inhibited by the extract/fractions. The extract also promoted the closure of scratch wounds in HaCaT cells. The K. alpina extract/fractions contained cardamonin, quercetin, and quercitrin.
Conclusion: K. alpina extracts/fractions showed antioxidant, antiaging, whitening, and anti-inflammatory activities, suggesting they may have potential as antiaging cosmeceuticals.
期刊介绍：
Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin.
Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered.
The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.
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