Julia H Majolo, João I B Gonçalves, Renata P Souza, Laura C González, Nathalia Sperotto, Maiele D Silveira, Sílvia D Oliveira, Cristiano V Bizarro, Pablo Machado, Luiz A Basso, Ana P D Souza, Jarbas R Oliveira, Carlos A S Ferreira
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引用次数: 0
摘要
背景:COVID-19 大流行给全球医疗保健系统带来了巨大挑战,尤其是对患有高血压等原有疾病的人造成了影响。本研究试图评估抗高血压药物洛沙坦和马来酸依那普利对 SARS-CoV-2 感染细胞的影响。对 Vero E6 细胞进行体外感染和处理,通过 MTT 比色法评估细胞活力。此外,研究还利用逆转录酶和实时定量聚合酶链反应测定了病毒 RNA 和选定基因信使 RNA 的相对水平:结果:研究结果表明,洛沙坦能将 SARS-CoV-2 的核头状 RNA 水平大幅降低到几乎检测不到的水平,而马来酸依那普利则没有明显效果。与未感染未处理的细胞相比,病毒感染后,il-18、p53、p21 和 p62 的表达均有所增加。值得注意的是,il-6的表达在感染和处理两种情况下均上调。用洛沙坦或马来酸依那普利处理的感染细胞之间的比较突显了il-6、p53、p21和p62表达的不同特征:我们的研究数据表明,这些药物可干扰 Vero E6 细胞感染 SARS-CoV-2 后产生的某些效应。结论:我们的研究数据表明,这些药物可干扰 SARS-CoV-2 感染 Vero E6 细胞所引发的某些效应,但它们对新陈代谢和信号转导途径的影响似乎在量和质上都有所不同。
Losartan and enalapril maleate differently influence SARS-CoV-2-infected vero cells.
Background: The COVID-19 pandemic has posed significant challenges to global healthcare systems, particularly impacting individuals with pre-existing conditions like hypertension. This study sought to assess the impact of the antihypertensive medications, losartan and enalapril maleate on SARS-CoV-2 infected cells. Vero E6 cells were infected and treated in vitro, evaluating cell viability via the MTT colorimetric assay. Additionally, the study measured relative levels of viral RNA and selected gene messenger RNAs using reverse transcriptase followed by quantitative real-time polymerase chain reaction.
Results: The findings revealed that losartan substantially reduced nucleocapsid RNA levels of SARS-CoV-2 to nearly undetectable levels, while enalapril maleate did not demonstrate a significant effect. In response to viral infection, the expression of il-18, p53, p21, and p62 increased compared to uninfected-untreated cells. Notably, il-6 expression was upregulated by both infection and treatments. A comparison between infected cells treated with losartan or enalapril maleate highlighted the presence of distinct profiles in the expression of il-6, p53, p21, and p62.
Conclusions: The data from our study suggest that these medications could interfere with certain effects triggered by SARS-CoV-2 infection in Vero E6 cells. However, their influence appears to vary both quantitatively and qualitatively in the modulation of metabolic and signal transduction pathways.
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