{"title":"一种便携式光学传感器,结合了智能手机和基于藻青素的荧光试纸,用于快速、可视和现场检测 CO32。","authors":"Yifeng Xu, Rui Sun, Shubo Wang, Fei Fei, Zhangyu Gan, Pin Zhou","doi":"10.1016/j.saa.2024.125276","DOIUrl":null,"url":null,"abstract":"<p><p>With the development of global industry, carbon dioxide emissions surged. The conversion of carbon dioxide from the air results in some CO<sub>3</sub><sup>2-</sup>, which can exacerbate environmental disasters like ocean acidification. Therefore, the content of CO<sub>3</sub><sup>2-</sup> in seawater is an important indicator of the degree of ocean acidification. In this study, natural fluorescent protein phycocyanin (PC) was used as a fluorescent probe, and a fluorescence detection method was established for quantitative monitoring of CO<sub>3</sub><sup>2-</sup> with quick response time (within 50 s), high sensitivity, and selectivity. The fluorescence quenching phenomenon between PC and CO<sub>3</sub><sup>2-</sup> was mainly attributed to static quenching. The limit of detection (LOD) was 0.42 μM and the method was successfully applied to monitor CO<sub>3</sub><sup>2-</sup> in tap water and seawater, acquiring satisfactory recovery between 99.28 % and 106.40 %. More importantly, paper-based test strips were easily fabricated using PC, enabling the rapid, visual, and on-site detection of CO<sub>3</sub><sup>2-</sup> with the aid of a smartphone. The visual detection integrated with the smartphone was converted to data information (RGB value) through a Color Picker APP and successfully used for quantitative identification of CO<sub>3</sub><sup>2-</sup>. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, the linear calibration ranged from 0.5 μM to 500.0 μM with LOD of 0.11 μM was obtained. Satisfactory recoveries were acquired in tap water (98.00 %-107.50 %) and seawater (97.30 %-101.74 %), respectively. Therefore, integrating the PC fluorescent paper with a smartphone realizes the rapid, visual, and on-site detection of CO<sub>3</sub><sup>2-</sup> in the water environment, which is expected to broaden application prospects of monitoring ocean acidification degree.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A portable optical sensor combining smartphone with phycocyanin-based fluorescent test paper for rapid, visual and on-site detection of CO<sub>3</sub><sup>2</sup>.\",\"authors\":\"Yifeng Xu, Rui Sun, Shubo Wang, Fei Fei, Zhangyu Gan, Pin Zhou\",\"doi\":\"10.1016/j.saa.2024.125276\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>With the development of global industry, carbon dioxide emissions surged. The conversion of carbon dioxide from the air results in some CO<sub>3</sub><sup>2-</sup>, which can exacerbate environmental disasters like ocean acidification. Therefore, the content of CO<sub>3</sub><sup>2-</sup> in seawater is an important indicator of the degree of ocean acidification. In this study, natural fluorescent protein phycocyanin (PC) was used as a fluorescent probe, and a fluorescence detection method was established for quantitative monitoring of CO<sub>3</sub><sup>2-</sup> with quick response time (within 50 s), high sensitivity, and selectivity. The fluorescence quenching phenomenon between PC and CO<sub>3</sub><sup>2-</sup> was mainly attributed to static quenching. The limit of detection (LOD) was 0.42 μM and the method was successfully applied to monitor CO<sub>3</sub><sup>2-</sup> in tap water and seawater, acquiring satisfactory recovery between 99.28 % and 106.40 %. More importantly, paper-based test strips were easily fabricated using PC, enabling the rapid, visual, and on-site detection of CO<sub>3</sub><sup>2-</sup> with the aid of a smartphone. The visual detection integrated with the smartphone was converted to data information (RGB value) through a Color Picker APP and successfully used for quantitative identification of CO<sub>3</sub><sup>2-</sup>. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, the linear calibration ranged from 0.5 μM to 500.0 μM with LOD of 0.11 μM was obtained. Satisfactory recoveries were acquired in tap water (98.00 %-107.50 %) and seawater (97.30 %-101.74 %), respectively. Therefore, integrating the PC fluorescent paper with a smartphone realizes the rapid, visual, and on-site detection of CO<sub>3</sub><sup>2-</sup> in the water environment, which is expected to broaden application prospects of monitoring ocean acidification degree.</p>\",\"PeriodicalId\":94213,\"journal\":{\"name\":\"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.saa.2024.125276\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.saa.2024.125276","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A portable optical sensor combining smartphone with phycocyanin-based fluorescent test paper for rapid, visual and on-site detection of CO32.
With the development of global industry, carbon dioxide emissions surged. The conversion of carbon dioxide from the air results in some CO32-, which can exacerbate environmental disasters like ocean acidification. Therefore, the content of CO32- in seawater is an important indicator of the degree of ocean acidification. In this study, natural fluorescent protein phycocyanin (PC) was used as a fluorescent probe, and a fluorescence detection method was established for quantitative monitoring of CO32- with quick response time (within 50 s), high sensitivity, and selectivity. The fluorescence quenching phenomenon between PC and CO32- was mainly attributed to static quenching. The limit of detection (LOD) was 0.42 μM and the method was successfully applied to monitor CO32- in tap water and seawater, acquiring satisfactory recovery between 99.28 % and 106.40 %. More importantly, paper-based test strips were easily fabricated using PC, enabling the rapid, visual, and on-site detection of CO32- with the aid of a smartphone. The visual detection integrated with the smartphone was converted to data information (RGB value) through a Color Picker APP and successfully used for quantitative identification of CO32-. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, the linear calibration ranged from 0.5 μM to 500.0 μM with LOD of 0.11 μM was obtained. Satisfactory recoveries were acquired in tap water (98.00 %-107.50 %) and seawater (97.30 %-101.74 %), respectively. Therefore, integrating the PC fluorescent paper with a smartphone realizes the rapid, visual, and on-site detection of CO32- in the water environment, which is expected to broaden application prospects of monitoring ocean acidification degree.