基于 Hg2+ 离子介导的双重保护红色发射铜纳米团簇,开发用于检测三聚氰胺的开关 "OFF-ON "荧光探针。

Ramadan Ali, Abdullah Alattar, Abdullah S Albalawi, Alanoud Alkhamali, Osama A Hakami, Hamzah Hussain Alharthi, Mohammed S Alahmari, Abdullah H Alharbi, Omar Mohammed Aljohani, Yahya A Yahya Alzahrani, Turki M Albaqami, Mohamed M El-Wekil
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引用次数: 0

摘要

三聚氰胺因其蛋白质含量高而常被用作婴幼儿配方奶粉的掺杂物,大量摄入会对人体造成危害,导致婴儿体内形成氰尿酸-三聚氰胺共晶体,并可能造成肾脏损伤。在这项研究中,我们介绍了一种荧光方法,用于选择性地、可靠地检测牛奶和婴儿配方奶粉中的三聚氰胺。该荧光探针是由硫代水杨酸(TSA)和聚乙烯吡咯烷酮(PVP)作为双重保护配体功能化的纳米铜簇(Cu NCs)组成。加入 Hg2+ 后,TSA-PVP@Cu NCs 的荧光发射会因静态淬灭而减弱。随后,由于三聚氰胺和 Hg2+ 之间的配位相互作用以及它们之间形成稳定的螯合物,TSA-PVP@Cu NCs + Hg2+ 探针的荧光发射在引入三聚氰胺后得以恢复。在优化条件下,首先记录 TSA-PVP@Cu NCs + Hg2+ 探针的荧光发射(F°),然后记录加入三聚氰胺后的荧光发射(F)。在 0.025-65 µM 范围内,随着三聚氰胺浓度的增加,(F/F°)比率也在增加。根据信噪比 3 计算得出的检测限为 8.0 nM。TSA-PVP@Cu NCs + Hg2+探针被成功用于检测牛奶和婴儿配方奶粉中的三聚氰胺,其回收率和相对标准偏差均可接受。这些结果证明了该探针用于实际样品中三聚氰胺荧光检测的可靠性和有效性。
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Developing a switch "OFF-ON" fluorescent probe for detection of melamine based on doubly-protected red emissive copper nanoclusters mediated by Hg2+ ions.

Melamine, often used as an adulterant in infants' formula due to its high protein content, can be harmful when ingested in large amounts, leading to the formation of cyanurate-melamine co-crystals in infants and potentially causing kidney damage. In this study, we introduce a fluorescent method for the selective and reliable detection of melamine in milk and infants' formula. The fluorescent probe comprises copper nanoclusters (Cu NCs) functionalized with thiosalicylic acid (TSA) and polyvinylpyrrolidone (PVP) as double-protecting ligands. Upon the addition of Hg2+, the fluorescence emission of TSA-PVP@Cu NCs is diminished due to static quenching. Subsequently, the fluorescence emission of the TSA-PVP@Cu NCs + Hg2+ probe is restored upon the introduction of melamine, facilitated by the coordination interaction between melamine and Hg2+ and the formation of a stable chelate between them. Under optimized conditions, the fluorescence emission was recorded initially for the TSA-PVP@Cu NCs + Hg2+ probe (F°) and after melamine addition (F). The (F/F°) ratio increased with rising melamine concentrations within the range of 0.025-65 µM. The detection limit, calculated using a signal-to-noise ratio of 3, was determined to be 8.0 nM. The TSA-PVP@Cu NCs + Hg2+ probe was successfully employed to detect melamine in milk and infants' formula, yielding acceptable recovery percentages and relative standard deviations. These results underscore the reliability and efficacy of the proposed probe for the fluorometric detection of melamine in real-world samples.

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