掺杂钼的碳点纳米酶具有过氧化物样活性,可用于灵敏、选择性的智能手机辅助比色法 S2- 离子检测和抗菌应用。

Dai Li, Huajie Chen, Yutao Zheng, Sheng Zhou, Fengyuan Yong, Xiangbo Zhang, Kui Wang, Huiyun Wen, Jiyong Wu, Weiming Xue, Saipeng Huang
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引用次数: 0

摘要

硫离子(S2-)在许多生物体和生态系统中发挥着重要的作用,而其异常含量会对人类健康和生态环境造成严重危害。因此,构建一个高灵敏度和高选择性的分析平台来检测复杂微环境,尤其是生物系统中的硫离子(S2-)是非常有意义的。本研究利用磷钼酸和 L-Arg 通过一步水热法制备了一种新型的掺钼碳点纳米酶(Mo-CDs),它具有很强的过氧化物酶样活性。在 H2O2 的存在下,Mo-CDs 能将 3,3',5,5'-四甲基联苯胺(TMB)转化为蓝色的 oxTMB,但 S2- 能将蓝色溶液强烈还原为无色,然后变为棕色,这表明 Mo-CDs 对 S2- 具有显著的选择性。通过紫外分析和智能手机辅助可视比色分析,Mo-CDs 具有宽线性范围(2.5 μM-900 μM)和低检测限(LOD = 76 nM)。特别是智能手机辅助分析平台成功实现了对水样中 S2- 的快速、便携、灵敏和可视鉴定,且回收率高(95.7-106.7 %),特异性好。更重要的是,基于良好的过氧化物酶样活性,Mo-CDs 被开发用于抗菌应用。该研究不仅构建了一种新型、高效的碳点纳米酶和一种低成本、便携式、可视化的实时检测 S2-的分析平台,而且提出了一种新的设计策略和方法来开发多功能纳米酶检测工具,具有重要的实际应用价值。
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Mo-doped carbon-dots nanozyme with peroxide-like activity for sensitive and selective smartphone-assisted colorimetric S2- ion detection and antibacterial application.

Sulfur ion (S2-) plays a significant and considerable role in many living organisms and ecosystems, while its abnormal content can pose a serious hazard to human health and ecological environment. Hence, it is extremely meaningful to construct a highly sensitive and selective analytical platform for S2- detection in complex microenvironment, particularly in biological systems. In this study, phosphomolybdic acid and L-Arg were utilized to prepare a new molybdenum doped carbon-dots nanozyme (Mo-CDs) with great peroxidase-like activity by one-step hydrothermal approach. In the presence of H2O2, Mo-CDs converted 3,3',5,5'-tetramethyl benzidine (TMB) into blue oxTMB, but S2- strongly reduced the blue solution to colorless and then brown, which established significant selectivity toward S2-. Mo-CDs illustrated a wide linear range (2.5 μM-900 μM) and low detection limit (LOD = 76 nM) by ultraviolet and smartphone-assisted visualized colorimetric analysis. Especially, the smartphone-assisted analysis platform successfully realized quick, portable, sensitive and visible identification of S2- with high recovery (95.7-106.7 %) and excellent specificity in water samples. More importantly, Mo-CDs was developed to antibacterial applications based on good peroxidase-like activity. This research not only constructed a new and efficient carbon-dots nanozyme and a low-cost, portable, visual analysis platform for real-time detection of S2-, but also proposed a novel design strategy and methodology for exploiting multifunctional nanozyme detection tool with great practical application.

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