{"title":"掺杂钼的碳点纳米酶具有过氧化物样活性,可用于灵敏、选择性的智能手机辅助比色法 S2- 离子检测和抗菌应用。","authors":"Dai Li, Huajie Chen, Yutao Zheng, Sheng Zhou, Fengyuan Yong, Xiangbo Zhang, Kui Wang, Huiyun Wen, Jiyong Wu, Weiming Xue, Saipeng Huang","doi":"10.1016/j.saa.2024.125274","DOIUrl":null,"url":null,"abstract":"<p><p>Sulfur ion (S<sup>2-</sup>) plays a significant and considerable role in many living organisms and ecosystems, while its abnormal content can pose a serious hazard to human health and ecological environment. Hence, it is extremely meaningful to construct a highly sensitive and selective analytical platform for S<sup>2-</sup> detection in complex microenvironment, particularly in biological systems. In this study, phosphomolybdic acid and L-Arg were utilized to prepare a new molybdenum doped carbon-dots nanozyme (Mo-CDs) with great peroxidase-like activity by one-step hydrothermal approach. In the presence of H<sub>2</sub>O<sub>2</sub>, Mo-CDs converted 3,3',5,5'-tetramethyl benzidine (TMB) into blue oxTMB, but S<sup>2-</sup> strongly reduced the blue solution to colorless and then brown, which established significant selectivity toward S<sup>2-</sup>. Mo-CDs illustrated a wide linear range (2.5 μM-900 μM) and low detection limit (LOD = 76 nM) by ultraviolet and smartphone-assisted visualized colorimetric analysis. Especially, the smartphone-assisted analysis platform successfully realized quick, portable, sensitive and visible identification of S<sup>2-</sup> with high recovery (95.7-106.7 %) and excellent specificity in water samples. More importantly, Mo-CDs was developed to antibacterial applications based on good peroxidase-like activity. This research not only constructed a new and efficient carbon-dots nanozyme and a low-cost, portable, visual analysis platform for real-time detection of S<sup>2-</sup>, but also proposed a novel design strategy and methodology for exploiting multifunctional nanozyme detection tool with great practical application.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"326 ","pages":"125274"},"PeriodicalIF":0.0000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mo-doped carbon-dots nanozyme with peroxide-like activity for sensitive and selective smartphone-assisted colorimetric S<sup>2-</sup> ion detection and antibacterial application.\",\"authors\":\"Dai Li, Huajie Chen, Yutao Zheng, Sheng Zhou, Fengyuan Yong, Xiangbo Zhang, Kui Wang, Huiyun Wen, Jiyong Wu, Weiming Xue, Saipeng Huang\",\"doi\":\"10.1016/j.saa.2024.125274\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sulfur ion (S<sup>2-</sup>) plays a significant and considerable role in many living organisms and ecosystems, while its abnormal content can pose a serious hazard to human health and ecological environment. Hence, it is extremely meaningful to construct a highly sensitive and selective analytical platform for S<sup>2-</sup> detection in complex microenvironment, particularly in biological systems. In this study, phosphomolybdic acid and L-Arg were utilized to prepare a new molybdenum doped carbon-dots nanozyme (Mo-CDs) with great peroxidase-like activity by one-step hydrothermal approach. In the presence of H<sub>2</sub>O<sub>2</sub>, Mo-CDs converted 3,3',5,5'-tetramethyl benzidine (TMB) into blue oxTMB, but S<sup>2-</sup> strongly reduced the blue solution to colorless and then brown, which established significant selectivity toward S<sup>2-</sup>. Mo-CDs illustrated a wide linear range (2.5 μM-900 μM) and low detection limit (LOD = 76 nM) by ultraviolet and smartphone-assisted visualized colorimetric analysis. Especially, the smartphone-assisted analysis platform successfully realized quick, portable, sensitive and visible identification of S<sup>2-</sup> with high recovery (95.7-106.7 %) and excellent specificity in water samples. More importantly, Mo-CDs was developed to antibacterial applications based on good peroxidase-like activity. This research not only constructed a new and efficient carbon-dots nanozyme and a low-cost, portable, visual analysis platform for real-time detection of S<sup>2-</sup>, but also proposed a novel design strategy and methodology for exploiting multifunctional nanozyme detection tool with great practical application.</p>\",\"PeriodicalId\":94213,\"journal\":{\"name\":\"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy\",\"volume\":\"326 \",\"pages\":\"125274\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.saa.2024.125274\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.saa.2024.125274","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mo-doped carbon-dots nanozyme with peroxide-like activity for sensitive and selective smartphone-assisted colorimetric S2- ion detection and antibacterial application.
Sulfur ion (S2-) plays a significant and considerable role in many living organisms and ecosystems, while its abnormal content can pose a serious hazard to human health and ecological environment. Hence, it is extremely meaningful to construct a highly sensitive and selective analytical platform for S2- detection in complex microenvironment, particularly in biological systems. In this study, phosphomolybdic acid and L-Arg were utilized to prepare a new molybdenum doped carbon-dots nanozyme (Mo-CDs) with great peroxidase-like activity by one-step hydrothermal approach. In the presence of H2O2, Mo-CDs converted 3,3',5,5'-tetramethyl benzidine (TMB) into blue oxTMB, but S2- strongly reduced the blue solution to colorless and then brown, which established significant selectivity toward S2-. Mo-CDs illustrated a wide linear range (2.5 μM-900 μM) and low detection limit (LOD = 76 nM) by ultraviolet and smartphone-assisted visualized colorimetric analysis. Especially, the smartphone-assisted analysis platform successfully realized quick, portable, sensitive and visible identification of S2- with high recovery (95.7-106.7 %) and excellent specificity in water samples. More importantly, Mo-CDs was developed to antibacterial applications based on good peroxidase-like activity. This research not only constructed a new and efficient carbon-dots nanozyme and a low-cost, portable, visual analysis platform for real-time detection of S2-, but also proposed a novel design strategy and methodology for exploiting multifunctional nanozyme detection tool with great practical application.