粘弹性溶液中大肠杆菌趋化效率的提高

IF 2.9 3区 化学 Q3 CHEMISTRY, PHYSICAL Soft Matter Pub Date : 2024-10-16 DOI:10.1039/D4SM01094A
Shaoying Zhu, Rui He, Caijuan Yue, Rongjing Zhang and Junhua Yuan
{"title":"粘弹性溶液中大肠杆菌趋化效率的提高","authors":"Shaoying Zhu, Rui He, Caijuan Yue, Rongjing Zhang and Junhua Yuan","doi":"10.1039/D4SM01094A","DOIUrl":null,"url":null,"abstract":"<p >Bacteria inhabit complex environments rich in macromolecular polymers that exhibit viscoelastic properties. While the influence of viscoelasticity on bacterial swimming is recognized, its impact on chemotaxis—a critical behavior for bacterial survival and colonization—remains elusive. In this study, we employed a microfluidic device to establish attractant gradients and observed the chemotactic behavior of <em>Escherichia coli</em> in both viscoelastic solutions containing carboxymethyl cellulose (CMC) and Newtonian buffers. Our results reveal that <em>E. coli</em> demonstrates markedly enhanced chemotactic efficiency in viscoelastic media. Notably, bacteria achieved faster migration velocities and higher steady-state accumulation in areas with higher attractant concentrations compared to those in Newtonian conditions. Through 3D tracking, we determined that changes in bulk motility parameters alone do not account for the observed enhancements. Further investigations through theoretical analysis and stochastic simulations suggested that the main enhancement mechanisms are mitigation of surface hydrodynamic hindrance resulting from solid surfaces commonly present in bacterial habitats, and the induction of a lifting force in viscoelastic solutions. These findings highlight the significant role of the rheological properties of bacterial habitats in shaping their chemotactic strategies, offering deeper insights into bacterial adaptive mechanisms in both natural and clinical settings.</p>","PeriodicalId":103,"journal":{"name":"Soft Matter","volume":" 43","pages":" 8675-8683"},"PeriodicalIF":2.9000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced chemotaxis efficiency of Escherichia coli in viscoelastic solutions†\",\"authors\":\"Shaoying Zhu, Rui He, Caijuan Yue, Rongjing Zhang and Junhua Yuan\",\"doi\":\"10.1039/D4SM01094A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Bacteria inhabit complex environments rich in macromolecular polymers that exhibit viscoelastic properties. While the influence of viscoelasticity on bacterial swimming is recognized, its impact on chemotaxis—a critical behavior for bacterial survival and colonization—remains elusive. In this study, we employed a microfluidic device to establish attractant gradients and observed the chemotactic behavior of <em>Escherichia coli</em> in both viscoelastic solutions containing carboxymethyl cellulose (CMC) and Newtonian buffers. Our results reveal that <em>E. coli</em> demonstrates markedly enhanced chemotactic efficiency in viscoelastic media. Notably, bacteria achieved faster migration velocities and higher steady-state accumulation in areas with higher attractant concentrations compared to those in Newtonian conditions. Through 3D tracking, we determined that changes in bulk motility parameters alone do not account for the observed enhancements. Further investigations through theoretical analysis and stochastic simulations suggested that the main enhancement mechanisms are mitigation of surface hydrodynamic hindrance resulting from solid surfaces commonly present in bacterial habitats, and the induction of a lifting force in viscoelastic solutions. These findings highlight the significant role of the rheological properties of bacterial habitats in shaping their chemotactic strategies, offering deeper insights into bacterial adaptive mechanisms in both natural and clinical settings.</p>\",\"PeriodicalId\":103,\"journal\":{\"name\":\"Soft Matter\",\"volume\":\" 43\",\"pages\":\" 8675-8683\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Soft Matter\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/sm/d4sm01094a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Soft Matter","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sm/d4sm01094a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

摘要

细菌栖息在富含大分子聚合物的复杂环境中,这些聚合物具有粘弹性。虽然粘弹性对细菌游动的影响已得到公认,但它对趋化性--细菌生存和定殖的关键行为--的影响却仍然难以捉摸。在这项研究中,我们利用微流体设备建立了吸引梯度,并观察了大肠杆菌在含有羧甲基纤维素(CMC)的粘弹性溶液和牛顿缓冲液中的趋化行为。我们的研究结果表明,大肠杆菌在粘弹性介质中的趋化效率明显提高。值得注意的是,与牛顿条件下的细菌相比,在吸引剂浓度较高的区域,细菌的迁移速度更快,稳态积累也更高。通过三维跟踪,我们确定仅凭体积运动参数的变化并不能解释所观察到的提高。通过理论分析和随机模拟进行的进一步研究表明,主要的增强机制是减轻细菌栖息地常见的固体表面所产生的表面流体动力阻碍,以及在粘弹性溶液中诱导提升力。这些发现凸显了细菌栖息地的流变特性在形成细菌趋化策略中的重要作用,为深入了解细菌在自然和临床环境中的适应机制提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Enhanced chemotaxis efficiency of Escherichia coli in viscoelastic solutions†

Bacteria inhabit complex environments rich in macromolecular polymers that exhibit viscoelastic properties. While the influence of viscoelasticity on bacterial swimming is recognized, its impact on chemotaxis—a critical behavior for bacterial survival and colonization—remains elusive. In this study, we employed a microfluidic device to establish attractant gradients and observed the chemotactic behavior of Escherichia coli in both viscoelastic solutions containing carboxymethyl cellulose (CMC) and Newtonian buffers. Our results reveal that E. coli demonstrates markedly enhanced chemotactic efficiency in viscoelastic media. Notably, bacteria achieved faster migration velocities and higher steady-state accumulation in areas with higher attractant concentrations compared to those in Newtonian conditions. Through 3D tracking, we determined that changes in bulk motility parameters alone do not account for the observed enhancements. Further investigations through theoretical analysis and stochastic simulations suggested that the main enhancement mechanisms are mitigation of surface hydrodynamic hindrance resulting from solid surfaces commonly present in bacterial habitats, and the induction of a lifting force in viscoelastic solutions. These findings highlight the significant role of the rheological properties of bacterial habitats in shaping their chemotactic strategies, offering deeper insights into bacterial adaptive mechanisms in both natural and clinical settings.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Soft Matter
Soft Matter 工程技术-材料科学:综合
CiteScore
6.00
自引率
5.90%
发文量
891
审稿时长
1.9 months
期刊介绍: Where physics meets chemistry meets biology for fundamental soft matter research.
期刊最新文献
Formation of bijels stabilized by magnetic ellipsoidal particles in external magnetic fields. Geometrical impacts of platonic particles on nematic liquid crystal dynamics. Rolling and ordering of micro rods in shear flow induced by rod wall interactions. Liquid crystalline collagen assemblies as substrates for directed alignment of human Schwann cells. Long-lived unidirectional flow of active particles within long narrow channels.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1