NaHS 可保护雌雄大鼠的大脑、心脏和肺等远端器官免受肾缺血/再灌注诱发的氧化应激的影响。

IF 2.2 4区 医学 Q2 UROLOGY & NEPHROLOGY BMC Nephrology Pub Date : 2024-10-22 DOI:10.1186/s12882-024-03824-3
Shadan Saberi, Hamid Najafipour, Mohammad Amin Rajizadeh, Abbas Etminan, Elham Jafari, Maryam Iranpour
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引用次数: 0

摘要

急性肾损伤(AKI)经常发生在重症监护病房的住院病人身上,通常是由肾缺血再灌注损伤(IRI)引起的。IRI 会通过炎症、氧化应激、细胞凋亡、白细胞浸润以及尿素和肌酐水平升高,破坏肺、胰腺、肠道、肝脏、心脏和大脑等各种 "远端器官 "的功能。肾脏 IRI 引起的损伤存在性别差异。H2S 是一种内源性气体调节剂,在血管扩张、支气管扩张和降血压方面具有潜力,并能调节细胞凋亡、炎症、血管生成、新陈代谢和氧化应激。本研究旨在探讨 NaHS 对肾红外损伤后脑、心和肺损伤的保护作用,并评估氧化系统状态作为一种潜在机制对雌雄大鼠的影响:48只Wistar大鼠被随机分为8组(n = 6):对照组/Saline组、Sham组/Saline组、IR组/Saline组和IR组/NaHS组。在 IR 组中,诱导双侧肾缺血 45 分钟,然后进行 24 小时再灌注。治疗组在钳夹释放前 10 分钟给予 NaHS(100µM/Kg,IP)。对作为远端器官的大脑、心脏和肺的 BUN、SCr、BUN/SCr、白蛋白尿、组织病理学和氧化应激生物标志物进行评估。红外线增加了肾功能血清标志物、白蛋白尿、丙二醛水平和组织损伤评分,同时降低了亚硝酸盐水平、超氧化物歧化酶和谷胱甘肽过氧化物酶活性。尽管 NaHS 对肾功能的改善有限,但它能逆转红外线对男女患者远处器官的不良影响。我们的研究结果表明,NaHS 可通过减轻氧化应激对肾脏红外损伤后的远端器官损伤产生有益的影响,并且在反应中存在明显的组织特异性和性别特异性差异。这些研究结果表明,NaHS 是减轻肾脏红外损伤后多器官损伤的潜在治疗药物,其效果因组织和性别而异。
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NaHS protects brain, heart, and lungs as remote organs from renal ischemia/reperfusion-induced oxidative stress in male and female rats.

Acute Kidney Injury (AKI) is frequently observed in hospitalized patients in intensive care units, often caused by renal ischemia-reperfusion injury (IRI). IRI disrupts the function of various 'remote organs' such as the lungs, pancreas, intestine, liver, heart, and brain through inflammation, oxidative stress, apoptosis, leukocyte infiltration, and increased urea and creatinine levels. Gender differences in renal IRI-induced injury are noted. H2S, an endogenous gaseous modulator, shows potential in vasodilation, bronchodilation, and hypotension and can regulate apoptosis, inflammation, angiogenesis, metabolism, and oxidative stress. This study aims to investigate the protective effects of NaHS on brain, heart, and lung injuries following renal IR and to assess the oxidative system status as a potential mechanism in male and female rats.Forty-eight Wistar rats were randomly divided into eight groups (n = 6): Control/Saline, Sham/Saline, IR/Saline, and IR/NaHS in both sexes. Forty-five minutes of bilateral renal ischemia followed by 24-hour reperfusion was induced in the IR groups. NaHS (100µM/Kg, IP) was administered 10 min before clamp release in treated groups. BUN, SCr, BUN/SCr, albuminuria, histopathology, and oxidative stress biomarkers of the brain, heart, and lung were assessed as remote organs. IR increased serum markers of renal function, albuminuria, malondialdehyde levels, and tissue injury scores while reducing nitrite levels and superoxide dismutase and glutathione peroxidase activities. NaHS treatment reversed the adverse effects of IR in remote organs in both sexes, although it showed limited improvement in renal function. Our findings demonstrate that NaHS has a beneficial effect on remote organ injury following renal IR by mitigating oxidative stress, with noted tissue-specific and gender-specific differences in response. These findings suggest NaHS as a potential therapeutic agent for mitigating multi-organ injury after renal IR, with effects varying by tissue and gender.

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来源期刊
BMC Nephrology
BMC Nephrology UROLOGY & NEPHROLOGY-
CiteScore
4.30
自引率
0.00%
发文量
375
审稿时长
3-8 weeks
期刊介绍: BMC Nephrology is an open access journal publishing original peer-reviewed research articles in all aspects of the prevention, diagnosis and management of kidney and associated disorders, as well as related molecular genetics, pathophysiology, and epidemiology.
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