通过对 Platycladus orientalis (L.) Franco 叶的正己烷萃取物和精油的化学成分、抗氧化性和酶抑制潜能的分子内研究。

IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry & Biodiversity Pub Date : 2024-10-27 DOI:10.1002/cbdv.202402000
Doaa A Korany, Nilofar Nilofar, Gokhan Zengin, Omayma A Eldahshan
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引用次数: 0

摘要

板蓝根叶在传统医学中被广泛用于治疗各种疾病。本研究通过正己烷萃取和水蒸馏法获得了挥发性成分。利用气相色谱-质谱分析进行了全面的代谢组学分析。此外,还对体外抗氧化潜力和酶抑制活性进行了评估,并得到了硅学分析的支持。结果表明,水蒸馏挥发油中主要是单萜烃类(42.30%),其次是含氧倍半萜类(32.10%);主要成分是柏木酚。二萜类化合物(49.70%)和倍半萜碳氢化合物(13.43%)是正己烷萃取物的主要成分;二萜醇 vulgarol A 是主要成分。挥发油在 ABTS 和 DDPH 清除活性、CUPRAC 和 FRAP 试验等所有试验中都表现出更高的抗氧化潜力。不过,正己烷提取物对丁酰胆硷酯酶、酪氨酸酶、α-淀粉酶和α-葡萄糖苷酶具有广泛的抑制作用,分子对接研究和预测性 ADME 分析也证明了这一点。因此,可以得出结论,正己烷提取物是治疗酶失调症的可行选择。此外,还可以推断出挥发油在制药业和氧化应激管理方面的潜在用途。这些结果值得进一步研究,以验证挥发油和正己烷提取物的治疗潜力。
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Chemical Constituents, Antioxidant, and Enzyme Inhibitory Potentials Supported by In-Silico Studies of the n-Hexane Extract and Essential Oil of Platycladus Orientalis (L.) Franco Leaves.

Platycladus orientalis leaves are widely used in traditional medicine to treat different ailments. In the present study, the volatile constituents were obtained by n-hexane extraction and hydrodistillation. Comprehensive metabolomic profiling was performed using GC-MS analysis. Furthermore, in vitro antioxidant potential and enzyme-inhibitory activity were assessed and supported by in silico profiling. Results revealed the predominance of monoterpene hydrocarbons in the hydrodistilled volatile oil (42.30 %) followed by oxygenated sesquiterpenes (32.10 %); with cedrol as the main component. Diterpenoids (49.70 %) and sesquiterpene hydrocarbons (13.43 %) were the major components of the n-hexane extract; with vulgarol A, a diterpene alcohol, as the major constituent. The volatile oil demonstrated significantly higher antioxidant potential across all assays, including ABTS and DDPH scavenging activity, CUPRAC, and FRAP assays. However, the n-hexane extract demonstrated broad inhibitory effects against butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase enzymes, supported by molecular docking study and predictive ADME profiling. Therefore, it may be concluded that the n-hexane extract is a viable option for treating dysregulated enzyme conditions. In addition, the potential use of volatile oil in the pharmaceutical industries and management of oxidative stress can be inferred. These results warrant further studies to validate the therapeutic potential of the volatile oil and the n-hexane extract.

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来源期刊
Chemistry & Biodiversity
Chemistry & Biodiversity 环境科学-化学综合
CiteScore
3.40
自引率
10.30%
发文量
475
审稿时长
2.6 months
期刊介绍: Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.
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