党参多糖的结构特征及与 THP-1 细胞免疫调节的结构-活性关系

IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry & Biodiversity Pub Date : 2024-10-25 DOI:10.1002/cbdv.202401167
Junjie Fu, Cong Chang, Jinyang Ren, Jingjing Cheng, Ziwei Gao, Yan Meng
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引用次数: 0

摘要

近年来,党参多糖因其在免疫调节方面的卓越表现而受到越来越多的关注。然而,其结构与免疫调节活性之间的关系却鲜有报道。本研究以 THP-1 细胞为研究对象,通过色谱柱分离纯化了四种粗提物(CPW、CPS0.2、CPS0.5、CPS1),探讨了它们的结构和免疫调节作用。结果表明,CPs 的单糖组成、链构象、分子量(Mw)和聚集状态各不相同。同时,CPs 的免疫调节作用也因结构差异而不同,LPS 诱导的 THP-1 细胞释放的 TNF-α 和 IL-1β 细胞因子也表明了这一点。最后,我们总结并得出结论:CPs 的球形结构和较小的粒径是其具有较好免疫增强效果的关键因素。结果表明,多糖的单糖组合差异可能会导致抗炎或促炎活性。此外,CPs 中较高的葡萄糖比例、相对较大的粒径以及延链构象可能是其具有较好免疫增强效果的关键因素。本研究旨在为建立氯化石蜡的结构-活性关系提供理论依据。
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The Structure Characterization of Polysaccharides from Codonopsis pilosula and the Structure-activity Relationship with Immune-regulation on THP-1 Cells.

In recent years, polysaccharides from Codonopsis pilosula (CPs) have received increasing attention for their excellent behaviors in immune-regulation. However, the relationship between the structure and immunomodulatory activity has rarely been reported. In this work, four fractions purified from crude CPs (CPW, CPS0.2, CPS0.5, CPS1) by chromatographic column separation were explored with both structure and immunomodulatory effects by THP-1 cells. The results showed that the monosaccharide composition, chain conformation, molecular weight (Mw) and aggregated state of CPs were different. At the same time, the immunomodulatory effects of CPs were also varied depend on structure differences, as indicated by the released cytokines of TNF-α and IL-1β by LPS-induced THP-1 cells. Finally, we summarized and concluded that the spherical structure and smaller particle size of CPs were the key factors attributed to better immune-enhancing effects. The results showed that the monosaccharide combination differences of polysaccharides might lead to anti-inflammatory or pro-inflammatory activity. Moreover, the higher percentage of glucose, the relatively large particle size, as well as the extended chain conformation of CPs might be the key factors attributed to better immune-enhancing effects. This study aims to provide a theoretical basis for establishing the structure-activity relationship of CPs.

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来源期刊
Chemistry & Biodiversity
Chemistry & Biodiversity 环境科学-化学综合
CiteScore
3.40
自引率
10.30%
发文量
475
审稿时长
2.6 months
期刊介绍: Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.
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