Arsh Bibi, Fathia Mubeen, Ali Rizwan, Irfan Ullah, Masooma Hammad, Muhammad Abu Bakar Waqas, Ayesha Ikram, Zaheer Abbas, Dennis Halterman, Nasir Ahmad Saeed
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Morphological characterization, <i>internal transcribed spacer</i> (<i>ITS</i>) sequencing, specific <i>translation elongation factor 1-alpha</i> (<i>TEF</i>) sequencing, and phylogenetic analysis were used to identify six different <i>Fusarium</i> species: <i>F. oxysporum</i>, <i>F. equiseti</i>, <i>F. incarnatum</i>, <i>F. fujikuroi</i>, <i>F. annulatum</i> and <i>F. thapsinum</i>. Pathogenicity tests in a greenhouse revealed that <i>F. oxysporum</i> and <i>F. equiseti</i> were responsible for Fusarium wilt in all sampled regions, with <i>F. oxysporum</i> being more prevalent in wilted samples. This is the first report of <i>F. equiseti</i> on wilted potatoes in Pakistan. In vitro biocontrol tests using <i>Trichoderma harzianum</i> showed 89% inhibition against <i>F. equiseti</i> and 65% inhibition against <i>F. oxysporum</i>. These findings on <i>F. equiseti</i> will aid in developing future control strategies, including biocontrol measures for Fusarium wilt in potatoes.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508693/pdf/","citationCount":"0","resultStr":"{\"title\":\"Morpho-Molecular Identification of <i>Fusarium equiseti</i> and <i>Fusarium oxysporum</i> Associated with Symptomatic Wilting of Potato from Pakistan.\",\"authors\":\"Arsh Bibi, Fathia Mubeen, Ali Rizwan, Irfan Ullah, Masooma Hammad, Muhammad Abu Bakar Waqas, Ayesha Ikram, Zaheer Abbas, Dennis Halterman, Nasir Ahmad Saeed\",\"doi\":\"10.3390/jof10100701\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Potato (<i>Solanum tuberosum</i> L.) is one of the emerging staple crops in Pakistan, with Punjab producing over 95% of the country's potatoes. Wilt is an emerging threat to the potato crop worldwide, including in Pakistan. We identified and characterized <i>Fusarium</i> species associated with potato wilt in Pakistan through morphological and molecular analyses. Samples were collected during the 2020-2022 potato seasons from five major potato-growing regions: Sahiwal, Chichawatni, Pakpattan, Kamalia, and Faisalabad. Morphological characterization, <i>internal transcribed spacer</i> (<i>ITS</i>) sequencing, specific <i>translation elongation factor 1-alpha</i> (<i>TEF</i>) sequencing, and phylogenetic analysis were used to identify six different <i>Fusarium</i> species: <i>F. oxysporum</i>, <i>F. equiseti</i>, <i>F. incarnatum</i>, <i>F. fujikuroi</i>, <i>F. annulatum</i> and <i>F. thapsinum</i>. Pathogenicity tests in a greenhouse revealed that <i>F. oxysporum</i> and <i>F. equiseti</i> were responsible for Fusarium wilt in all sampled regions, with <i>F. oxysporum</i> being more prevalent in wilted samples. This is the first report of <i>F. equiseti</i> on wilted potatoes in Pakistan. In vitro biocontrol tests using <i>Trichoderma harzianum</i> showed 89% inhibition against <i>F. equiseti</i> and 65% inhibition against <i>F. oxysporum</i>. These findings on <i>F. equiseti</i> will aid in developing future control strategies, including biocontrol measures for Fusarium wilt in potatoes.</p>\",\"PeriodicalId\":15878,\"journal\":{\"name\":\"Journal of Fungi\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508693/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fungi\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/jof10100701\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fungi","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/jof10100701","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
马铃薯(Solanum tuberosum L.)是巴基斯坦新兴的主食作物之一,旁遮普省的马铃薯产量占全国产量的 95% 以上。枯萎病是对包括巴基斯坦在内的全球马铃薯作物的新威胁。我们通过形态学和分子分析确定了与巴基斯坦马铃薯枯萎病相关的镰刀菌种并对其进行了定性。我们在 2020-2022 年马铃薯收获季节从五个主要马铃薯种植区采集了样本:萨希瓦尔(Sahiwal)、奇查瓦特尼(Chichawatni)、帕克帕坦(Pakpattan)、卡马利亚(Kamalia)和费萨拉巴德(Faisalabad)。通过形态特征描述、内部转录隔距(ITS)测序、特异性翻译延伸因子 1-α(TEF)测序和系统发育分析,确定了六个不同的镰刀菌种:F. oxysporum、F. equiseti、F. incarnatum、F. fujikuroi、F. annulatum 和 F. thapsinum。在温室中进行的致病性测试表明,在所有采样地区,镰孢菌枯萎病都是由 F. oxysporum 和 F. equiseti 引起的,其中 F. oxysporum 在枯萎样本中更为普遍。这是巴基斯坦首次报告马铃薯枯萎病中的马镰孢菌。使用 Harzianum 毛霉进行的体外生物防治试验显示,对 F. equiseti 的抑制率为 89%,对 F. oxysporum 的抑制率为 65%。这些关于马铃薯镰刀菌枯萎病的研究结果将有助于制定未来的控制策略,包括针对马铃薯镰刀菌枯萎病的生物控制措施。
Morpho-Molecular Identification of Fusarium equiseti and Fusarium oxysporum Associated with Symptomatic Wilting of Potato from Pakistan.
Potato (Solanum tuberosum L.) is one of the emerging staple crops in Pakistan, with Punjab producing over 95% of the country's potatoes. Wilt is an emerging threat to the potato crop worldwide, including in Pakistan. We identified and characterized Fusarium species associated with potato wilt in Pakistan through morphological and molecular analyses. Samples were collected during the 2020-2022 potato seasons from five major potato-growing regions: Sahiwal, Chichawatni, Pakpattan, Kamalia, and Faisalabad. Morphological characterization, internal transcribed spacer (ITS) sequencing, specific translation elongation factor 1-alpha (TEF) sequencing, and phylogenetic analysis were used to identify six different Fusarium species: F. oxysporum, F. equiseti, F. incarnatum, F. fujikuroi, F. annulatum and F. thapsinum. Pathogenicity tests in a greenhouse revealed that F. oxysporum and F. equiseti were responsible for Fusarium wilt in all sampled regions, with F. oxysporum being more prevalent in wilted samples. This is the first report of F. equiseti on wilted potatoes in Pakistan. In vitro biocontrol tests using Trichoderma harzianum showed 89% inhibition against F. equiseti and 65% inhibition against F. oxysporum. These findings on F. equiseti will aid in developing future control strategies, including biocontrol measures for Fusarium wilt in potatoes.
期刊介绍:
Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.