使用吲哚菁绿负载的脆性钠多糖和壳聚糖纳米粒子的光免疫疗法可抑制肿瘤生长和转移。

IF 10.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Nanobiotechnology Pub Date : 2024-10-23 DOI:10.1186/s12951-024-02944-0
Dayoung Ryu, Hae-Bin Park, Eun-Koung An, So-Jung Kim, Da Young Kim, Daeun Lim, Juyoung Hwang, Minseok Kwak, Wonpil Im, Ja-Hyoung Ryu, SangGuan You, Peter C W Lee, Jun-O Jin
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引用次数: 0

摘要

转移和复发是癌症治疗的主要挑战。在各种治疗方法中,免疫疗法有望预防转移和复发。在这项研究中,我们评估了光免疫治疗纳米粒子治疗原发性癌症和阻断转移和复发的疗效,这些纳米粒子是用两种带电多糖合成的。脆性钠多糖(CFP)带负电荷,具有免疫刺激特性,与带正电荷的壳聚糖结合合成纳米粒子。此外,还在这些颗粒中加入了光敏剂吲哚菁绿(ICG),称为壳聚糖-CFP-ICG(CC-ICG)。小鼠结肠癌细胞(CT-26)内化了 CC-ICG,随后 808 纳米激光照射促进了细胞凋亡/坏死。此外,小鼠瘤内注射 CC-ICG 并用 808 纳米激光照射可消除 CT-26 肿瘤。CC-ICG治愈的小鼠在树突状细胞激活介导的细胞毒性T淋巴细胞刺激下,CT-26癌的再侵袭肺转移受到抑制。这些结果表明,CC-ICG 是一种天然的肿瘤治疗药物,具有治疗原发性肿瘤、抑制转移和复发的潜力。
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Photoimmunotherapy using indocyanine green-loaded Codium fragile polysaccharide and chitosan nanoparticles suppresses tumor growth and metastasis.

Metastasis and recurrence are the main challenges in cancer treatment. Among various therapeutic approaches, immunotherapy holds promise for preventing metastasis and recurrence. In this study, we evaluated the efficacy of treating primary cancer and blocking metastasis and recurrence with photo-immunotherapeutic nanoparticles, which were synthesized using two types of charged polysaccharides. Codium fragile polysaccharide (CFP), which exhibits immune-stimulating properties and carries a negative charge, was combined with positively charged chitosan to synthesize nanoparticles. Additionally, indocyanine green (ICG), a photosensitizer, was loaded inside these particles and was referred to as chitosan-CFP-ICG (CC-ICG). Murine colon cancer cells (CT-26) internalized CC-ICG, and subsequent 808-nanometer laser irradiation promoted apoptotic/necrotic cell death. Moreover, intratumoral injection of CC-ICG, with 808-nanometer laser irradiation eliminated CT-26 tumors in mice. Rechallenged lung metastases of CT-26 cancer were inhibited by dendritic cell activation-mediated cytotoxic T lymphocyte stimulation in mice cured by CC-ICG. These results demonstrated that CC-ICG is a natural tumor therapeutic with the potential to treat primary tumors and suppress metastasis and recurrence.

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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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