用液化二甲醚直接提取湿微藻杜纳藻中的脂质、β-胡萝卜素和多酚类化合物

IF 4.9 2区 医学 Q1 CHEMISTRY, MEDICINAL Marine Drugs Pub Date : 2024-09-26 DOI:10.3390/md22100438
Hideki Kanda, Kaito Kusumi, Li Zhu, Tao Wang
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引用次数: 0

摘要

从高湿微藻中提取脂质和高价值产品需要大量能源用于干燥预处理。在这项研究中,我们考察了利用液化二甲醚(DME)直接从湿的盐生杜莎藻中提取脂质、β-胡萝卜素和多酚化合物的方法。二甲醚提取的β-胡萝卜素含量为 7.0 毫克/克,高于氯仿-甲醇提取的β-胡萝卜素含量。此外,二甲醚萃取的总酚含量及其抗氧化能力也略高于氯仿-甲醇萃取。二甲醚几乎去除了所有水分,萃取了 29.2 重量百分比的总脂类和 9.7 重量百分比的脂肪酸。与氯仿-甲醇萃取相比,液化二甲醚从湿样品中萃取的脂质更多。用二甲醚萃取的脂质的 C/N 比为 112.0,高于氯仿-甲醇萃取的 C/N 比。高 C/N 比表明,液化二甲醚提取含氮磷脂酰胆碱可能不那么容易,而且可能具有高度选择性。然而,饱和脂肪酸的比率为 34.8%,低于氯仿-甲醇的比率。培养基中的 Na+ 和 Mg2+ 没有被二甲醚萃取出来。因此,与使用氯仿-甲醇提取相比,使用二甲醚提取有利有弊,但其提取性能令人满意。二甲醚不需要传统萃取溶剂所必须的干燥过程,因此有望成为一种环境友好型替代溶剂。
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Direct Extraction of Lipids, β-Carotene, and Polyphenolic Compounds from Wet Microalga Dunaliella salina by Liquefied Dimethyl Ether.

Extraction of lipids and high-value products from highly wet microalgae requires significant energy for the drying pretreatment. In this study, we examined the direct extraction of lipids, β-carotene, and polyphenolic compounds from wet Dunaliella salina using liquefied dimethyl ether (DME), which is effective in lipid extraction for biofuel production. The amount of DME-extracted β-carotene was 7.0 mg/g, which was higher than that obtained from the chloroform-methanol extraction. Moreover, the total phenolic content extracted with DME and its antioxidant capacity were slightly higher than those extracted with chloroform-methanol. DME removed almost all the water and extracted 29.2 wt% of total lipids and 9.7 wt% of fatty acids. More lipids were extracted from wet samples by liquefied DME than by chloroform-methanol extraction. The C/N ratio of lipids extracted with DME was 112.0, higher than that of chloroform-methanol. The high C/N ratio suggests that nitrogen-containing phosphatidylcholines may be less easily extracted by liquefied DME and may be highly selective. However, the ratio of saturated fatty acids was 34.8%, lower than that of chloroform-methanol. Na+ and Mg2+ in the culture medium were not extracted using DME. Thus, using the extract with DME has both advantages and disadvantages compared to using the extract with chloroform-methanol; however, it has satisfactory extraction properties. DME is expected to be an environment-friendly alternative solvent because it does not require drying, which is necessary for conventional extraction solvents.

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来源期刊
Marine Drugs
Marine Drugs 医学-医药化学
CiteScore
9.60
自引率
14.80%
发文量
671
审稿时长
1 months
期刊介绍: Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.
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