Panjie Sheng, Baochao Bai, Mingjian Liu, Weiqin Ma, Jianliang Liu, Chaoran Song, Shuai Du, Gentu Ge, Yushan Jia, Zhijun Wang
{"title":"不同添加剂和比例对帚状高粱秸秆青贮特性和细菌群落的影响","authors":"Panjie Sheng, Baochao Bai, Mingjian Liu, Weiqin Ma, Jianliang Liu, Chaoran Song, Shuai Du, Gentu Ge, Yushan Jia, Zhijun Wang","doi":"10.3390/microorganisms12102062","DOIUrl":null,"url":null,"abstract":"<p><p>As a large agricultural country, China produces a large number of agricultural and sideline products while harvesting agricultural products every year. Crop straw is one of them. Broom sorghum is a traditional crop in China, which produces a large amount of straw resources every year. These straw resources are placed in the field and cannot be used efficiently. The purpose of this study was to solve the problem of straw utilization of Broom sorghum, one of the main food crops in arid and semi-arid areas of northern China. Broom sorghum is not only a nutritious food crop, its straw is also rich in crude fiber and mineral elements, which has high utilization value. However, due to the high content of lignocellulose in straw, the texture is hard, which limits its digestion and utilization efficiency as feed. In this study, the broom sorghum straw was used as the research object, and the straw raw materials were treated with <i>Lactobacillus plantarum</i>, cellulase and xylanase, respectively. After silage fermentation for 30 d and 60 d, the bags were opened to determine the nutritional quality, fermentation quality, microbial community structure and other indicators. The best fermentation time and additives for broom sorghum straw silage were comprehensively screened to improve the nutritional value of straw and animal production performance. The results showed that the nutritional quality of silage straw increased with the extension of fermentation time. Compared with silage straw after 30 days of fermentation, the nutritional quality and fermentation quality of straw were significantly improved after 60 days of fermentation. <i>Lactobacillus plantarum</i>, cellulase and xylanase could improve the silage performance of broom sorghum straw by improving the microbial community structure in straw, and the effect of cellulase was the best. When cellulase was used in straw at the standard of 20 U/g FM, the content of water-soluble carbohydrates could be significantly increased to 31.35 g/kg FM, and the concentration of lactic acid was also significantly increased to 23.79 g/kg FM. Therefore, in actual production, it is recommended to use cellulase at a dose of 20 U/g FM in broom sorghum silage and open the bag after 60 days of silage fermentation. The results of this study provided a scientific basis for the efficient utilization of broom sorghum straw as feed.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 10","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509844/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effects of Different Additives and Ratios on Broom Sorghum Straw Silage Characteristics and Bacterial Communities.\",\"authors\":\"Panjie Sheng, Baochao Bai, Mingjian Liu, Weiqin Ma, Jianliang Liu, Chaoran Song, Shuai Du, Gentu Ge, Yushan Jia, Zhijun Wang\",\"doi\":\"10.3390/microorganisms12102062\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>As a large agricultural country, China produces a large number of agricultural and sideline products while harvesting agricultural products every year. Crop straw is one of them. Broom sorghum is a traditional crop in China, which produces a large amount of straw resources every year. These straw resources are placed in the field and cannot be used efficiently. The purpose of this study was to solve the problem of straw utilization of Broom sorghum, one of the main food crops in arid and semi-arid areas of northern China. Broom sorghum is not only a nutritious food crop, its straw is also rich in crude fiber and mineral elements, which has high utilization value. However, due to the high content of lignocellulose in straw, the texture is hard, which limits its digestion and utilization efficiency as feed. In this study, the broom sorghum straw was used as the research object, and the straw raw materials were treated with <i>Lactobacillus plantarum</i>, cellulase and xylanase, respectively. After silage fermentation for 30 d and 60 d, the bags were opened to determine the nutritional quality, fermentation quality, microbial community structure and other indicators. The best fermentation time and additives for broom sorghum straw silage were comprehensively screened to improve the nutritional value of straw and animal production performance. The results showed that the nutritional quality of silage straw increased with the extension of fermentation time. Compared with silage straw after 30 days of fermentation, the nutritional quality and fermentation quality of straw were significantly improved after 60 days of fermentation. <i>Lactobacillus plantarum</i>, cellulase and xylanase could improve the silage performance of broom sorghum straw by improving the microbial community structure in straw, and the effect of cellulase was the best. When cellulase was used in straw at the standard of 20 U/g FM, the content of water-soluble carbohydrates could be significantly increased to 31.35 g/kg FM, and the concentration of lactic acid was also significantly increased to 23.79 g/kg FM. Therefore, in actual production, it is recommended to use cellulase at a dose of 20 U/g FM in broom sorghum silage and open the bag after 60 days of silage fermentation. 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引用次数: 0
摘要
作为一个农业大国,中国在每年收获农产品的同时,也会生产出大量的农副产品。农作物秸秆就是其中之一。帚状高粱是中国的传统农作物,每年都会产生大量的秸秆资源。这些秸秆资源被放置在田间地头,无法得到有效利用。帚状高粱是中国北方干旱和半干旱地区的主要粮食作物之一,本研究旨在解决帚状高粱的秸秆利用问题。帚状高粱不仅是一种营养丰富的粮食作物,其秸秆还富含粗纤维和矿物质元素,具有很高的利用价值。然而,由于秸秆中木质纤维素含量较高,质地较硬,限制了其作为饲料的消化和利用效率。本研究以扫帚高粱秸秆为研究对象,分别用植物乳杆菌、纤维素酶和木聚糖酶处理秸秆原料。青贮发酵 30 d 和 60 d 后,开袋测定营养质量、发酵质量、微生物群落结构等指标。综合筛选出锦高粱秸秆青贮的最佳发酵时间和添加剂,以提高秸秆的营养价值和动物生产性能。结果表明,青贮秸秆的营养品质随着发酵时间的延长而提高。与发酵 30 天后的青贮秸秆相比,发酵 60 天后秸秆的营养质量和发酵质量均有显著提高。植物乳杆菌、纤维素酶和木聚糖酶可通过改善秸秆中的微生物群落结构来提高扫帚高粱秸秆的青贮性能,其中纤维素酶的效果最好。当纤维素酶在秸秆中的使用标准为 20 U/g FM 时,水溶性碳水化合物的含量可显著提高到 31.35 g/kg FM,乳酸的浓度也显著提高到 23.79 g/kg FM。因此,在实际生产中,建议在帚状高粱青贮中使用纤维素酶,剂量为 20 U/g FM,青贮发酵 60 天后开袋。该研究结果为有效利用扫帚高粱秸秆作为饲料提供了科学依据。
Effects of Different Additives and Ratios on Broom Sorghum Straw Silage Characteristics and Bacterial Communities.
As a large agricultural country, China produces a large number of agricultural and sideline products while harvesting agricultural products every year. Crop straw is one of them. Broom sorghum is a traditional crop in China, which produces a large amount of straw resources every year. These straw resources are placed in the field and cannot be used efficiently. The purpose of this study was to solve the problem of straw utilization of Broom sorghum, one of the main food crops in arid and semi-arid areas of northern China. Broom sorghum is not only a nutritious food crop, its straw is also rich in crude fiber and mineral elements, which has high utilization value. However, due to the high content of lignocellulose in straw, the texture is hard, which limits its digestion and utilization efficiency as feed. In this study, the broom sorghum straw was used as the research object, and the straw raw materials were treated with Lactobacillus plantarum, cellulase and xylanase, respectively. After silage fermentation for 30 d and 60 d, the bags were opened to determine the nutritional quality, fermentation quality, microbial community structure and other indicators. The best fermentation time and additives for broom sorghum straw silage were comprehensively screened to improve the nutritional value of straw and animal production performance. The results showed that the nutritional quality of silage straw increased with the extension of fermentation time. Compared with silage straw after 30 days of fermentation, the nutritional quality and fermentation quality of straw were significantly improved after 60 days of fermentation. Lactobacillus plantarum, cellulase and xylanase could improve the silage performance of broom sorghum straw by improving the microbial community structure in straw, and the effect of cellulase was the best. When cellulase was used in straw at the standard of 20 U/g FM, the content of water-soluble carbohydrates could be significantly increased to 31.35 g/kg FM, and the concentration of lactic acid was also significantly increased to 23.79 g/kg FM. Therefore, in actual production, it is recommended to use cellulase at a dose of 20 U/g FM in broom sorghum silage and open the bag after 60 days of silage fermentation. The results of this study provided a scientific basis for the efficient utilization of broom sorghum straw as feed.
期刊介绍:
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.