在人子宫内膜基质细胞系(THESC)中表达的Nesfatin-1通过FAK/PI3K/AKT信号通路刺激蜕膜化。

IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Peptides Pub Date : 2024-10-24 DOI:10.1016/j.peptides.2024.171315
Jinah Ha, Hyunwon Yang
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引用次数: 0

摘要

本研究旨在探讨传统上与食欲调节有关的肽类激素内司芬-1在人类子宫内膜中的表达和功能作用。具体来说,我们研究了它在人类子宫内膜基质细胞系THESC细胞中的存在和调控潜力,重点关注子宫内膜蜕膜化过程,该过程对着床和维持妊娠至关重要。我们发现,nesfatin-1及其结合位点在THESC细胞中表达。此外,17β-雌二醇处理后,nesfatin-1蛋白表达量减少,但黄体酮处理后,nesfatin-1蛋白表达量增加,这表明类性激素对nesfatin-1的表达有影响。值得注意的是,给THESC细胞注射nesfatin-1蛋白会导致与蜕膜化相关的基因(如胰岛素样生长因子结合蛋白1(IGFBP1)和催乳素)显著上调。此外,我们的研究还表明,nesfatin-1能通过激活FAK/PI3K/AKT信号通路促进蜕膜化。这些发现强调了内司蛋白-1在子宫内膜蜕膜化中的核心作用,并表明它在开发新的治疗方法以改善生育能力和妊娠结局方面具有潜在的用途。
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Nesfatin-1 expressed in human endometrial stromal cell line (THESC) stimulates decidualization through FAK/PI3K/AKT signaling pathway
This study aimed to investigate the expression and functional role of nesfatin-1, a peptide hormone traditionally associated with appetite regulation, in the human endometrium. Specifically, we examined its presence and regulatory potential in the human endometrial stromal cell line, THESC cells, focusing on the process of endometrial decidualization, which is critical for implantation and pregnancy maintenance. We found that nesfatin-1 and its binding sites were expressed in THESC cells. Furthermore, nesfatin-1 protein expression decreased after treatment with 17β-estradiol but increased upon exposure to progesterone, indicating an influence of sexsteroid hormones on nesfatin-1 expression. Notably, administration of nesfatin-1 protein to THESC cells resulted in significant upregulation of genes associated with decidualization, such as insulin-like growth factor binding protein 1 (IGFBP1) and prolactin. In addition, our research showed that nesfatin-1 promotes decidualization through the activation of the FAK/PI3K/AKT signaling pathway. These findings underscore the central role of nesfatin-1 in endometrial decidualization, and suggest its potential utility in the development of new treatments to improve fertility and pregnancy outcomes.
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来源期刊
Peptides
Peptides 医学-生化与分子生物学
CiteScore
6.40
自引率
6.70%
发文量
130
审稿时长
28 days
期刊介绍: Peptides is an international journal presenting original contributions on the biochemistry, physiology and pharmacology of biological active peptides, as well as their functions that relate to gastroenterology, endocrinology, and behavioral effects. Peptides emphasizes all aspects of high profile peptide research in mammals and non-mammalian vertebrates. Special consideration can be given to plants and invertebrates. Submission of articles with clinical relevance is particularly encouraged.
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