从野生型鸡肠道中直接饲喂微生物群的分离、潜在有益特性和储存稳定性评估

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Probiotics and Antimicrobial Proteins Pub Date : 2024-10-25 DOI:10.1007/s12602-024-10387-0
Haiku D J Gómez-Velázquez, Pamela Peña-Medellín, Carlos O Guzmán-Hernández, Laura González-Dávalos, Alfredo Varela-Echavarría, Armando Shimada, Ofelia Mora
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引用次数: 0

摘要

在家禽生产中,直接饲喂微生物(DFM)因其健康益处而被视为抗生素类生长促进剂的替代品。然而,DFM 应经过严格的安全性测试,以确保其符合 "公认安全 "的标准。本研究评估了从野生型鸡的回肠和盲肠中分离出来的八个细菌群(BC),并对它们进行了益生菌测试。随后,对它们进行喷雾干燥和冷冻干燥,以评估其 30 天的储存稳定性。从回肠中分离出的 BC5-I 和 BC7-I 是很有前途的 DFM,它们在选择性培养基中显示出较高的乳酸菌含量,对革兰氏阳性和革兰氏阴性抗生素的敏感性也较高。这些 BC 对温度(> 90%)、pH 值 > 4(88-98%)有较高的耐受性,对大肠杆菌和沙门氏菌有拮抗作用。BC5-I 在模拟胃条件下的存活率更高,肠粘液粘附性也令人满意。冷冻干燥是获得 BC5-I 和 BC7-I 粉末的最佳方法,与开始储存时相比,存活率分别为 80.3% 和 73.2%。通过存活贮存动力学,BC5-I 的细胞死亡率最低,半衰期最长。BC5-I 只含有乳酸杆菌,而在液体(78.3%)和冻干(59.8%)形式中,Limosilactobacillus reuteri 是最主要的菌种。BC5-I 是一种以乳酸菌为基础的有前景的 DFM,可改善鸡肠道健康并提高肉蛋产量。
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Isolation, Potential Beneficial Properties, and Assessment of Storage Stability of Direct-Fed Microbial Consortia from Wild-Type Chicken Intestine.

Direct-fed microorganisms (DFM) are recognized as an alternative to antibiotic-based growth promoters in poultry production due to their health benefits. DFM, however, should undergo rigorous safety testing to ensure they meet the criteria to be "Generally Recognized as Safe." This study assessed eight bacterial consortia (BC) isolated from the ileal and cecal intestinal regions of wild-type chickens, subjecting them to probiotic tests. Subsequently, they were spray- and freeze-dried to evaluate their storage stability for 30 days. BC5-I and BC7-I, isolated from the ileum, emerged as promising DFM, displaying a high content of Lactobacillales using a selective medium and higher susceptibility to Gram-positive and Gram-negative antibiotics. These BC showed a high tolerance to temperature (> 90%), pH > 4 (88-98%), and antagonist effects against Escherichia coli and Salmonella. BC5-I exhibited superior survival in the simulated gastric conditions and satisfactory intestine mucus adhesion. Freeze-drying was the best method to obtain BC5-I and BC7-I powders, with a survival efficiency of 80.3% and 73.2%, respectively, compared to the beginning of storage. BC5-I presented the lowest cell death rate and prolonged half-life through survival storage kinetics. BC5-I only contained Lactobacillus, and Limosilactobacillus reuteri was the predominant species in liquid (78.3%) and freeze-dried (59.8%) forms. BC5-I stands out as a promising Lactobacillus-based DFM that could improve chicken intestinal health and enhance meat and egg production.

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来源期刊
Probiotics and Antimicrobial Proteins
Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY
CiteScore
11.30
自引率
6.10%
发文量
140
期刊介绍: Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.
期刊最新文献
The Influence of Protein Secretomes of Enterococcus durans on ex vivo Human Gut Microbiome. Lactobacillus rhamnosus GG Regulates Host IFN-I Through the RIG-I Signalling Pathway to Inhibit Herpes Simplex Virus Type 2 Infection. Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota. A Two Bacteriocinogenic Ligilactobacillus Strain Association Inhibits Growth, Adhesion, and Invasion of Salmonella in a Simulated Chicken Gut Environment. Gum Arabic/Chitosan Coacervates for Encapsulation and Protection of Lacticaseibacillus rhamnosus in Storage and Gastrointestinal Environments.
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