利用 CRISPR/Cas9 系统建立杂合子 LMOD2 基因敲除人类胚胎干细胞系（ZZUNEUi022-A-1）。

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Stem cell research Pub Date : 2024-10-19 DOI:10.1016/j.scr.2024.103586

Chunjin Zhang , Jing Li , Yipu Sai , Haitao Su , Youxu Jiang , Lihua Zhang , Liguo Jian , Hui Zhang , Guangli Guo , En Li , Xiaowei Li , Liqiang Sun

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引用次数: 0

摘要

扩张型心肌病（DCM）是一种常见的心肌病，以心室扩张和收缩功能障碍为特征。其病因错综复杂，包括多种遗传和环境因素。LMOD2（Leiomodin 2）基因已被证实与 DCM 的发病机制密切相关。本研究通过敲除 LMOD2 基因产生了纯细胞系，并通过诱导分化建立了 DCM 细胞模型，从而为进一步了解 DCM 的发病机制提供了有力的实验方法。这也为 DCM 的早期诊断和个体化治疗提供了一个潜在的研究方向。

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Establishment of heterozygous LMOD2 knockout human embryonic stem cell line (ZZUNEUi022-A-1) using CRISPR/Cas9 system

Dilated Cardiomyopathy (DCM), a prevalent form of cardiomyopathy, is characterized by ventricular dilation and systolic dysfunction. Its etiology is intricate, encompassing multiple genetic and environmental elements. The LMOD2 (Leiomodin 2) gene has been demonstrated to be closely associated with the pathogenesis of DCM. In this study, a pure cell line was generated by knocking out the LMOD2 gene, and a DCM cell model was established through induced differentiation, thus providing a powerful experimental approach for further understanding the pathogenesis of DCM. It also provides a potential research orientation for the early diagnosis and individualized treatment of DCM.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.