施用褪黑素纳米颗粒可改善夏季热应激下青春期前公羊的睾丸血流量、睾丸实质的回声纹理、阴囊周长以及雌二醇和一氧化氮水平。

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-10-23 DOI:10.1007/s11259-024-10563-1
Eman Fayez, Haney Samir, Fady Sayed Youssef, Ali Salama, Mohamed Ai ElSayed
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引用次数: 0

摘要

环境热应激(HS)会损害农场动物的繁殖效率。本研究首次研究了褪黑素和褪黑素纳米颗粒处理如何影响炎热气候条件下青春期前奥西米公羊的睾丸血液动力学、睾丸体积、回声纹理[像素强度(PIX)和综合密度(IND)]、阴囊周长、血清中睾酮(T)、雌二醇(E2)、一氧化氮(NO)浓度和总抗氧化能力(TAC)。接受检查的羔羊的温度湿度指数(THI)为 87.05 ± 1.70,表明其处于严重的 HS 状态。15 只青春期前的奥西米公羊接受了纳米褪黑素(纳米褪黑素组;20 毫克/克;n = 5)或悬浮在两毫升玉米油中的褪黑素(褪黑素组;40 毫克/克;n = 5)或两毫升玉米油(对照组;n = 5)的单次静脉注射。在治疗前(W0)立即对睾丸和睾丸上动脉(STA)进行采血和超声波评估,在注射纳米褪黑素和褪黑素后连续六周(W1-W6)每周进行一次评估。结果显示,与对照组相比,治疗组的变化有所减小(P 0.05)。E2、NO和TAC浓度增加(P
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Administration of melatonin nanoparticles improves testicular blood flow, echotexture of testicular parenchyma, scrotal circumference, and levels of estradiol and nitric oxide in prepubertal ossimi rams under summer heat stress.

Environmental heat stress (HS) impairs reproductive efficiency in farm animals. This study investigated, for the first time, how the melatonin and melatonin nanoparticles treatment affected the testicular hemodynamics, testicular volume, echotexture [Pixel intensity (PIX) and integrated density (IND)], scrotal circumference, serum concentration of testosterone (T), estradiol (E2), nitric oxide (NO), and total antioxidant capacity (TAC) in prepubertal Ossimi ram lambs in hot climatic conditions. The lambs undergoing examination had a temperature humidity index (THI) of 87.05 ± 1.70, indicating severe HS condition. Fifteen prepubertal Ossimi ram lambs were exposed to a single s.c injection of either nano melatonin (nano melatonin group; 20 mg/ram; n = 5) or melatonin suspended in two ml of corn oil (melatonin group; 40 mg/ram; n = 5) or two ml of corn oil (control group; n = 5). Blood collection and ultrasonographic assessment of the testes and supratesticular arteries (STAs) were conducted immediately before treatment (W0) and once weekly for six successive weeks after nano melatonin and melatonin injection (W1-W6). Results revealed decreases (P < 0.05) in the Doppler indices (resistive index; RI and pulsatility index; PI) of the testicular arteries at most time points of the study in the nano melatonin and melatonin groups. PIX of testicular parenchyma was significantly increased (P ˂ 0.05) in the treated groups compared to the control one. IND of testicular parenchyma increased significantly in the nano melatonin group compared to the melatonin and control groups. Testicular volume and scrotal circumference significantly increased (P < 0.05) in nano melatonin and melatonin groups compared to the control one. T concentration did not significantly (P > 0.05) change in the treated groups compared to the control group. E2, NO, and TAC concentrations increased (P < 0.05) in the treated groups compared to the control one. In conclusion, this study extrapolated that administrations of melatonin or nano melatonin can ameliorate the effects of environmental HS in prepubertal Ossimi ram lambs with a more protective effect and lower dose of nano melatonin.

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来源期刊
Veterinary Research Communications
Veterinary Research Communications 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
173
审稿时长
3 months
期刊介绍: Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
期刊最新文献
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