Xiaoying Shan , Shuang Chen , Aiying Bai , Yuwen Shi , Xuanli Song , Xiaoyu Yin , Chunhong Duan , Jinglei Tang , Xian Xia , Lanzheng Liu , Bingqing Zhu
{"title":"健康人群咽部奈瑟菌的动态携带情况。","authors":"Xiaoying Shan , Shuang Chen , Aiying Bai , Yuwen Shi , Xuanli Song , Xiaoyu Yin , Chunhong Duan , Jinglei Tang , Xian Xia , Lanzheng Liu , Bingqing Zhu","doi":"10.1016/j.meegid.2024.105684","DOIUrl":null,"url":null,"abstract":"<div><div>Considering the significant role of commensal <em>Neisseria</em> carried in the pharynx on the variation of <em>N.meningitidis</em> and the acquisition of its resistance genes, understanding the true <em>Neisseria</em> population colonizing the human pharynx is of great significance. In this study, we carried out a five-month continuous survey of oropharyngeal carriage in a certain healthy population to reveal the long-term carriage status of different <em>Neisseria</em> species. Totally, 419 <em>Neisseria</em> strains were isolated from 203 out of 205 pharyngeal swabs of 49 participants. Using combined methods (MALDI-TOF-MS, <em>rplF</em> sequencing and genome sequencing), the isolates were identified as <em>N.subflava</em> (<em>n</em> = 290), <em>N.mucosa</em> (<em>n</em> = 52), <em>N.oralis</em> (<em>n</em> = 8), <em>N.elongata</em> group (<em>n</em> = 6) and non-species-confirmed (<em>n</em> = 63). <em>N.subflava</em> was isolated from all individuals and 168 swabs (81.95 %). <em>N.mucosa</em>, <em>N.oralis, N.elongata</em> and non-species-confirmed were isolated from 25 (45), 6 (7), 4 (5) and 20 (53) individuals (swabs) respectively. It was common that multiple <em>Neisseria</em> spp. or multiple clones of one species were isolated from a single sample. An identical strain could be isolated frequently from a single person within five months. These results indicate that <em>Neisseria</em> spp. and <em>N.subflava</em> are ubiquitous in human pharynx and both have diverse population; we should pay more attention to them when studying <em>N.meningitidis</em> or other respiratory pathogens; robust and handy method for identifying <em>Neisseria</em> species remains to be developed.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"125 ","pages":"Article 105684"},"PeriodicalIF":2.6000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dynamic pharyngeal carriage of Neisseria species in healthy population\",\"authors\":\"Xiaoying Shan , Shuang Chen , Aiying Bai , Yuwen Shi , Xuanli Song , Xiaoyu Yin , Chunhong Duan , Jinglei Tang , Xian Xia , Lanzheng Liu , Bingqing Zhu\",\"doi\":\"10.1016/j.meegid.2024.105684\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Considering the significant role of commensal <em>Neisseria</em> carried in the pharynx on the variation of <em>N.meningitidis</em> and the acquisition of its resistance genes, understanding the true <em>Neisseria</em> population colonizing the human pharynx is of great significance. In this study, we carried out a five-month continuous survey of oropharyngeal carriage in a certain healthy population to reveal the long-term carriage status of different <em>Neisseria</em> species. Totally, 419 <em>Neisseria</em> strains were isolated from 203 out of 205 pharyngeal swabs of 49 participants. Using combined methods (MALDI-TOF-MS, <em>rplF</em> sequencing and genome sequencing), the isolates were identified as <em>N.subflava</em> (<em>n</em> = 290), <em>N.mucosa</em> (<em>n</em> = 52), <em>N.oralis</em> (<em>n</em> = 8), <em>N.elongata</em> group (<em>n</em> = 6) and non-species-confirmed (<em>n</em> = 63). <em>N.subflava</em> was isolated from all individuals and 168 swabs (81.95 %). <em>N.mucosa</em>, <em>N.oralis, N.elongata</em> and non-species-confirmed were isolated from 25 (45), 6 (7), 4 (5) and 20 (53) individuals (swabs) respectively. It was common that multiple <em>Neisseria</em> spp. or multiple clones of one species were isolated from a single sample. An identical strain could be isolated frequently from a single person within five months. These results indicate that <em>Neisseria</em> spp. and <em>N.subflava</em> are ubiquitous in human pharynx and both have diverse population; we should pay more attention to them when studying <em>N.meningitidis</em> or other respiratory pathogens; robust and handy method for identifying <em>Neisseria</em> species remains to be developed.</div></div>\",\"PeriodicalId\":54986,\"journal\":{\"name\":\"Infection Genetics and Evolution\",\"volume\":\"125 \",\"pages\":\"Article 105684\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Genetics and Evolution\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567134824001357\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Genetics and Evolution","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567134824001357","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Dynamic pharyngeal carriage of Neisseria species in healthy population
Considering the significant role of commensal Neisseria carried in the pharynx on the variation of N.meningitidis and the acquisition of its resistance genes, understanding the true Neisseria population colonizing the human pharynx is of great significance. In this study, we carried out a five-month continuous survey of oropharyngeal carriage in a certain healthy population to reveal the long-term carriage status of different Neisseria species. Totally, 419 Neisseria strains were isolated from 203 out of 205 pharyngeal swabs of 49 participants. Using combined methods (MALDI-TOF-MS, rplF sequencing and genome sequencing), the isolates were identified as N.subflava (n = 290), N.mucosa (n = 52), N.oralis (n = 8), N.elongata group (n = 6) and non-species-confirmed (n = 63). N.subflava was isolated from all individuals and 168 swabs (81.95 %). N.mucosa, N.oralis, N.elongata and non-species-confirmed were isolated from 25 (45), 6 (7), 4 (5) and 20 (53) individuals (swabs) respectively. It was common that multiple Neisseria spp. or multiple clones of one species were isolated from a single sample. An identical strain could be isolated frequently from a single person within five months. These results indicate that Neisseria spp. and N.subflava are ubiquitous in human pharynx and both have diverse population; we should pay more attention to them when studying N.meningitidis or other respiratory pathogens; robust and handy method for identifying Neisseria species remains to be developed.
期刊介绍：
(aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID)
Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance.
However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors.
Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases.
Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .
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