加工引起的何首乌滇黄酮含量减少和毒性：超高效液相色谱三重四极杆质谱分析和毒理学评估的启示。

IF 3.4 Q1 Health Professions Animal models and experimental medicine Pub Date : 2024-10-22 DOI:10.1002/ame2.12474

Wan-Fang Li, Ying Wang, Cai-Xia Qiu, Jie Li, Jie Bao, Jian-Bo Yang, Hong-Tao Jin

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摘要

背景：何首乌诱导的肝损伤（PM-DILI）严重阻碍了其临床应用和发展：何首乌诱导的肝损伤（PM-DILI）严重阻碍了其临床应用和发展：本研究调查了不同加工周期中何首乌毒性成分滇黄酮（dianthrones）的含量和毒性变化。我们采用了超高效液相色谱三重四极杆质谱法，对未加工的多花丹和采用九次蒸晒法加工的配方中的六种二蒽进行了定量分析。此外，还利用人体正常肝细胞系 L02 和斑马鱼胚胎进行了毒性评估：结果表明，随着加工周期的增加，二蒽含量逐渐减少。加工后的制剂对 L02 细胞的细胞毒性和对斑马鱼胚胎的肝毒性明显降低：我们的研究结果阐明了加工周期与多花金银花毒性之间的关系，为安全使用多花金银花提供了理论支持。

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Processing-induced reduction in dianthrones content and toxicity of Polygonum multiflorum: Insights from ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis and toxicological assessment

Background

Polygonum multiflorum-induced liver injury (PM-DILI) has significantly hindered its clinical application and development.

Methods

This study investigates the variation in content and toxicity of dianthrones, the toxic components of P. multiflorum, during different processing cycles. We employed the ultra-high-performance liquid chromatography triple quadrupole mass spectrometry method to quantify six dianthrones in raw P. multiflorum and formulations processed with a method called nine cycles of steaming and sunning. Additionally, toxicity assessments were conducted using human normal liver cell line L02 and zebrafish embryos.

Results

Results indicate a gradual reduction in dianthrones content with increasing processing cycles. Processed formulations exhibited significantly reduced cytotoxicity in L02 cells and hepatotoxicity in zebrafish embryos.