利用 HPLC-MS/MS 比较分析不同加工的附子煎煮提取物中的六种乌头类生物碱及其在大鼠体内的药代动力学行为。

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS Biomedical Chromatography Pub Date : 2024-10-28 DOI:10.1002/bmc.6035
Baodong Feng, Bing Shang, Yang Yang, Renyan Liu, Linqi Su, Yu Zhang, Lingyi Xin, Qinhua Chen, Zhihao Li
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引用次数: 0

摘要

Aconiti Lateralis Radix praeparata(又称 "附子")已被广泛应用于临床。然而,毒性问题限制了附子的治疗范围。因此,本研究开发了一种快速、灵敏的 HPLC-MS/MS 方法,用于同时定量比较四种不同加工的附子煎煮提取物中的六种活性/毒性成分以及大鼠口服附子煎煮提取物后血浆中的六种活性/毒性成分。验证了该方法的选择性、线性、灵敏度、精确度、准确度、基质效应、提取回收率和稳定性。采用HPLC-MS/MS对四种附子煎煮提取物中的六种生物碱进行了比较分析。采用主成分分析法(PCA)和正交偏最小二乘判别分析法(OPLS-DA)比较了不同夫子制品煎煮提取物的差异。此外,以黑顺片为代表,研究了其主要生物碱口服后的药代动力学行为。所建立的HPLC-MS/MS方法可同时分析煎煮提取物中的这些乌头类生物碱及其口服后的药代动力学行为,可为夫子的质量控制和临床处方的安全性与合理性监测铺平道路。
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Comparative analysis of six aconitine-type alkaloids in decocting extracts between the different processed Fuzi (Aconiti Lateralis Radix praeparata) and its pharmacokinetic behavior in rats by HPLC-MS/MS

Aconiti Lateralis Radix praeparata (also known as Fuzi in Chinese) has been extensively used in clinic. However, the toxicity issues limit the therapeutic range of Fuzi. Thus, a rapid and sensitive HPLC-MS/MS method was developed to simultaneously quantify and compare six active/toxic constituents in decocting extracts from four different processed Fuzi products and in rat plasma after oral administration of its decocting extracts. The selectivity, linearity, sensitivity, precisions, accuracy, matrix effects, extraction recoveries, and stability were validated. The comparative analysis of six alkaloids in decocting extracts between the four kinds of Fuzi products were conducted by the validated HPLC-MS/MS. Principal component analysis (PCA) and orthogonal partial least-squares discriminate analysis (OPLS-DA) were adopted to compare the differences of decocting extracts from the different processed Fuzi products. Besides, selecting Heishunpian (HSP) as the representative of the processed Fuzi products, we investigated the pharmacokinetic behaviors of these major alkaloids after oral administration. The developed HPLC-MS/MS method to simultaneously analyze these aconitine-type alkaloids in decocting extracts, and its pharmacokinetic behavior after oral administration may pave a way for quality control of Fuzi and monitoring the safety and rationality of clinical prescriptions.

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来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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