白藜芦醇在肾缺血再灌注损伤中的肾保护作用:对 Sprague-Dawley 大鼠的临床前研究。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY BMC Pharmacology & Toxicology Pub Date : 2024-10-28 DOI:10.1186/s40360-024-00809-8
Elaf R Alaasam, Ali M Janabi, Karrar M Al-Buthabhak, Rihab H Almudhafar, Najah R Hadi, Athanasios Alexiou, Marios Papadakis, Mohammed E Abo-El Fetoh, Dalia Fouad, Gaber El-Saber Batiha
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引用次数: 0

摘要

背景:肾缺血再灌注损伤(IRI)是导致肾功能障碍、急性肾损伤(AKI)以及相关发病率和死亡率的重要因素。白藜芦醇是一种多酚和植物毒素,以其抗炎、抗氧化和抗癌特性而闻名。本研究探讨了白藜芦醇在肾脏IRI大鼠模型中的肾脏保护潜力:将 28 只雄性 Sprague-Dawley 大鼠分为四组:白藜芦醇。Sham 组进行相同的手术,但不夹闭肾蒂;IRI 组缺血 30 分钟,然后再灌注 2 小时。DMSO组在缺血前30分钟腹腔注射二甲基亚砜(DMSO),白藜芦醇组在缺血前30分钟腹腔注射30毫克/千克白藜芦醇。评估生化指标(尿素、肌酐、IL-1β、NF-κβ、SOD、GSH、Bcl-2和caspase-3)和组织病理学变化:IRI导致血清肌酐、尿素、IL-1β、NF-κβ和caspase-3水平大幅升高,同时降低了SOD、GSH和Bcl-2水平。白藜芦醇治疗通过降低炎症和凋亡标志物、增强抗氧化防御能力和改善组织学结果减轻了这些影响:结论:白藜芦醇主要通过其抗氧化、抗炎和抗细胞凋亡特性,对肾脏IRI具有显著的肾保护作用。
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Nephroprotective role of resveratrol in renal ischemia-reperfusion injury: a preclinical study in Sprague-Dawley rats.

Background: Renal ischemia-reperfusion injury (IRI) is a significant contributor to renal dysfunction, acute kidney injury (AKI), and associated morbidity and mortality. Resveratrol, a polyphenol and phytoalexin, is known for its anti-inflammatory, antioxidant, and anti-cancer properties. This study investigates the nephroprotective potential of resveratrol in a rat model of renal IRI.

Materials and methods: Twenty-eight male Sprague-Dawley rats were divided into four groups: Sham, IRI, DMSO, and Resveratrol. The Sham group underwent identical procedures without renal pedicle clamping, while the IRI group experienced 30 min of ischemia followed by 2 h of reperfusion. The DMSO group received dimethyl sulfoxide (DMSO) intraperitoneally 30 min before ischemia, and the Resveratrol group received 30 mg/kg resveratrol intraperitoneally 30 min before ischemia. Biochemical parameters (Urea, creatinine, IL-1β, NF-κβ, SOD, GSH, Bcl-2, and caspase-3) and histopathological changes were assessed.

Results: IRI caused a substantial increase in serum creatinine, Urea, IL-1β, NF-κβ, and caspase-3 levels, while simultaneously decreasing SOD, GSH, and Bcl-2 levels. Resveratrol treatment mitigated these effects by lowering inflammatory and apoptotic markers, enhancing antioxidant defenses, and improving histological outcomes.

Conclusion: Resveratrol demonstrates significant nephroprotective effects in renal IRI, primarily through its antioxidant, anti-inflammatory, and anti-apoptotic properties.

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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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