Khristina G Judan Cruz, Okamoto Takumi, Kenneth A Bongulto, Emmanuel E Gandalera, Ngure Kagia, Kozo Watanabe
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This paper presents the inhibition of biofilm formation, the expression of biofilm-linked genes and ARGs by phytochemicals andrographolide, docosanol, lanosterol, quercetin, rutin and thymohydroquinone. <i>Aeromonas</i> species were isolated and purified from activated sludge samples. The ARGs were detected in the isolated <i>Aeromonas s</i>pecies through PCR. <i>Aeromonas</i> biofilms were quantified following the application of biocompounds through the microtiter plate assay. qPCR analyses of related genes were done for confirmation. Findings showed that the natural compounds inhibited the formation of biofilms and reduced the expression of genes linked to biofilm production as well as ARGs in wastewater <i>Aeromonas</i>. This indicates the efficacy of these compounds in targeting and controlling both ARGs and biofilm formation, highlighting their potential as innovative solutions for combating antimicrobial resistance and biofouling.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1456700"},"PeriodicalIF":4.6000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513397/pdf/","citationCount":"0","resultStr":"{\"title\":\"Natural compound-induced downregulation of antimicrobial resistance and biofilm-linked genes in wastewater <i>Aeromonas</i> species.\",\"authors\":\"Khristina G Judan Cruz, Okamoto Takumi, Kenneth A Bongulto, Emmanuel E Gandalera, Ngure Kagia, Kozo Watanabe\",\"doi\":\"10.3389/fcimb.2024.1456700\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Addressing the global antimicrobial resistance (AMR) crisis requires a multifaceted innovative approach to mitigate impacts on public health, healthcare and economic systems. 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引用次数: 0
摘要
应对全球抗菌药物耐药性(AMR)危机需要采取多方面的创新方法,以减轻对公共卫生、医疗保健和经济系统的影响。在 AMR 的复杂演变过程中,生物膜和抗菌药耐药性基因(ARGs)的获得起着关键作用。气单胞菌是一种主要的 AMR 感染者,它经常形成生物膜、携带 ARGs 并经常在废水中被检测到。现有的污水处理厂(WWTPs)没有能力完全消除耐抗菌素细菌,这有利于废水中 ARGs 的进化。除了促进 AMR 的出现外,生物膜还对污水处理厂生物反应器活性污泥中的生物污垢过程起着重要作用。本文介绍了植物化学物质穿心莲内酯、二十二烷醇、羊毛甾醇、槲皮素、芦丁和胸腺氢醌对生物膜形成、生物膜相关基因和 ARGs 表达的抑制作用。从活性污泥样本中分离并纯化了气单胞菌。通过聚合酶链反应在分离出的气单胞菌中检测到 ARGs。使用生物化合物后,通过微孔板测定法对气单胞菌生物膜进行定量。研究结果表明,天然化合物抑制了生物膜的形成,并减少了与生物膜生成有关的基因以及废水中气单胞菌 ARGs 的表达。这表明这些化合物在靶向和控制 ARGs 和生物膜形成方面具有功效,凸显了它们作为抗菌剂耐药性和生物污损的创新解决方案的潜力。
Natural compound-induced downregulation of antimicrobial resistance and biofilm-linked genes in wastewater Aeromonas species.
Addressing the global antimicrobial resistance (AMR) crisis requires a multifaceted innovative approach to mitigate impacts on public health, healthcare and economic systems. In the complex evolution of AMR, biofilms and the acquisition of antimicrobial resistance genes (ARGs) play a pivotal role. Aeromonas is a major AMR player that often forms biofilm, harbors ARGs and is frequently detected in wastewater. Existing wastewater treatment plants (WWTPs) do not have the capacity to totally eliminate antimicrobial-resistant bacteria favoring the evolution of ARGs in wastewater. Besides facilitating the emergence of AMR, biofilms contribute significantly to biofouling process within the activated sludge of WWTP bioreactors. This paper presents the inhibition of biofilm formation, the expression of biofilm-linked genes and ARGs by phytochemicals andrographolide, docosanol, lanosterol, quercetin, rutin and thymohydroquinone. Aeromonas species were isolated and purified from activated sludge samples. The ARGs were detected in the isolated Aeromonas species through PCR. Aeromonas biofilms were quantified following the application of biocompounds through the microtiter plate assay. qPCR analyses of related genes were done for confirmation. Findings showed that the natural compounds inhibited the formation of biofilms and reduced the expression of genes linked to biofilm production as well as ARGs in wastewater Aeromonas. This indicates the efficacy of these compounds in targeting and controlling both ARGs and biofilm formation, highlighting their potential as innovative solutions for combating antimicrobial resistance and biofouling.
期刊介绍:
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.