lncRNA LINC-PINT 在骨折延迟愈合中的功能机制和临床意义

IF 2.1 4区 医学 Q2 SURGERY Journal of Investigative Surgery Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI:10.1080/08941939.2024.2421826
Xiaoyu Ma, Xin Qian, Rong Ren, Yuzhou Chen, Hongyun Zhang, Ruirui Hao, Xinwei Pu, Yongliang Wang, Zhonglin Lu, Chao Tang
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引用次数: 0

摘要

背景:骨折愈合会受到各种因素的阻碍甚至破坏,导致越来越多的患者遭受痛苦。lncRNA LINC-PINT 因其在促进骨折愈合中的潜在作用而备受关注,但其在这一过程中的具体功能仍不清楚:本研究的主要目的是探讨 LINC-PINT 在骨折延迟愈合(DFH)中的临床意义和潜在分子机制,同时评估其作为早期诊断生物标志物的潜力:材料: 采用 RT-qPCR 方法检测了 LINC-PINT 在 DFH 患者和骨折愈合正常者血清中的表达水平。在 MC3T3-E1 细胞中,研究通过调控 LINC-PINT 和 miR-324-3p 对分化相关蛋白的表达、细胞活力和细胞凋亡的影响。为了阐明LINC-PINT、miR-324-3p和BMP2之间的靶向关系,研究采用了双荧光素酶报告实验:结果:研究结果显示,LINC-PINT在DFH患者中的表达明显下调。LINC-PINT作为DFH的诊断标志物具有很高的灵敏度和特异性。在 MC3T3-E1 细胞中,LINC-PINT 的过表达明显提高了 ALP、OCN、Runx2 和 OPN 的表达水平,改善了细胞活力并抑制了细胞凋亡。LINC-PINT 负向调节 miR-324-3p,而 miR-324-3p 则抵消了 LINC-PINT 的作用。结论:LINC-PINT可通过靶向miR-324-3p调节BMP2:结论:LINC-PINT可作为DFH的早期诊断生物标志物,并通过靶向调控miR-324-3p来调节BMP2,从而延缓DFH的进展。这项研究为诊断和治疗 DFH 提供了新的分子靶点。
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Functional Mechanism and Clinical Implications of lncRNA LINC-PINT in Delayed Fracture Healing.

Background: Fracture healing can be impeded or even compromised by various factors, resulting in a growing number of patients suffering. The lncRNA LINC-PINT has garnered attention for its latent role in enhancing fracture healing, but its specific functions in this process remain unclear.

Objectives: The primary objective of this study is to investigate the clinical relevance and underlying molecular mechanisms of LINC-PINT in delayed fracture healing (DFH), while also assessing its potential as an early diagnostic biomarker.

Materials and methods: The expression levels of LINC-PINT were measured in the serum of DFH patients and those with normal fracture healing using RT-qPCR. In MC3T3-E1 cells, the study investigated the influence on the expression of differentiation-related protein, cell viability, and apoptosis through the modulation of LINC-PINT and miR-324-3p. To elucidate the targeting relationship between LINC-PINT, miR-324-3p, and BMP2, a dual-luciferase reporter assay was employed.

Results: The findings revealed a significant downregulation of LINC-PINT expression in DFH patients. LINC-PINT showed high sensitivity and specificity as a diagnostic marker for DFH. In MC3T3-E1 cells, LINC-PINT overexpression markedly enhanced the expression levels of ALP, OCN, Runx2, and OPN, improved cell viability, and inhibited apoptosis. LINC-PINT negatively regulated miR-324-3p, and the effects of LINC-PINT were counteracted by miR-324-3p. LINC-PINT was found to regulate BMP2 by targeting miR-324-3p.

Conclusion: LINC-PINT could serve as an early diagnostic biomarker for DFH and slow the progression of DFH by modulating BMP2 through the targeted regulation of miR-324-3p. This research presents new molecular targets for the diagnosis and treatment of DFH.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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