感染沙眼衣原体后间质干细胞多能性标志物的丧失

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-12-17 DOI:10.4014/jmb.2406.06023
Munir A Al-Zeer, Mohammad Abu Lubad
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引用次数: 0

摘要

细胞内病原体沙眼衣原体可对宿主造成重大损害。值得注意的是,衣原体感染因其精确调节多种宿主信号通路以确保细胞存活而得到公认,这一现象与宿主细胞中的基因调控变化密切相关。为了监测衣原体感染细胞内基因调控的变化,我们采用间充质干细胞(MSCs)作为原始细胞模型。利用生化方法和成像技术,我们的研究发现,人间叶干细胞受到衣原体急性感染后,转录因子 Oct4、Sox2 和 Nanog 会下调,这表明多能性标志物丧失。相反,用受感染间充质干细胞的条件培养基处理后,间充质干细胞中的多能性标记得以维持。此外,在急性感染期间还观察到碱性磷酸酶活性增强以及 Sox9 和 CD44 mRNA 表达水平升高。利用触控 PCR 对特定细胞标记进行的全面筛选表明,早期软骨形成基因 Sox9 的 mRNA 上调,而间叶干细胞标记波形蛋白的 mRNA 则下降。实时 PCR 定量进一步证实了基因表达的改变,包括 Sox9 和 CD44 mRNA 水平的升高以及碱性磷酸酶活性的增强。总之,间充质干细胞感染沙眼衣原体后会诱发多种基因失调,这意味着间充质干细胞有向软骨细胞分化的潜在趋势。这些发现共同强调了衣原体对宿主细胞基因调控的定向影响,对这些细胞的最终命运和分化具有重要意义。
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Loss in Pluripotency Markers in Mesenchymal Stem Cells upon Infection with Chlamydia trachomatis.

The intracellular pathogen Chlamydia trachomatis can inflict substantial damage on the host. Notably, Chlamydia infection is acknowledged for its precise modulation of diverse host signaling pathways to ensure cell survival, a phenomenon intricately connected to genetic regulatory changes in host cells. To monitor shifts in gene regulation within Chlamydia-infected cells, we employed mesenchymal stem cells (MSCs) as a naïve, primary cell model. Utilizing biochemical methods and imaging, our study discloses that acute Chlamydia infection in human MSCs leads to the downregulation of transcription factors Oct4, Sox2, and Nanog, suggesting a loss of pluripotency markers. Conversely, pluripotency markers in MSCs were sustained through treatment with conditioned medium from infected MSCs. Additionally, there is an augmentation in alkaline phosphatase activity, along with elevated Sox9 and CD44 mRNA expression levels observed during acute infection. A comprehensive screening for specific cell markers using touchdown PCR indicates an upregulation of mRNA for the early chondrogenesis gene Sox9 and a decrease in mRNA for the MSC marker vimentin. Real-time PCR quantification further corroborates alterations in gene expression, encompassing increased Sox9 and CD44 mRNA levels, alongside heightened alkaline phosphatase activity. In summary, the infection of MSCs with C. trachomatis induces numerous genetic deregulations, implying a potential trend towards differentiation into chondrocytes. These findings collectively underscore a targeted impact of Chlamydia on the gene regulations of host cells, carrying significant implications for the final fate and differentiation of these cells.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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