马哈拉施特拉邦 Barvi 水库中感染 Ompok bimaculatus 和 Nemacheilus anguilla 的线虫寄生虫的 DNA 条形码

IF 0.7 4区 农林科学 Q4 FISHERIES Journal of Applied Ichthyology Pub Date : 2024-10-27 DOI:10.1155/2024/9644023
Gowhar Iqbal, Annam Pavan Kumar, Amjad Khansaheb Balange, Sanath Kumar, K. V. Rajendran, Sonal Suman, Nahida Quyoom, Sangeetha S., Showkat Ahmad Dar
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引用次数: 0

摘要

本研究对马哈拉施特拉邦巴维水库中感染 Ompok bimaculatus 和 Nemacheilus anguilla 鱼类的线虫寄生虫进行了 DNA 条形编码。为了确定这些线虫寄生虫的分类地位,使用了 18S 基因标记。准确鉴定鱼类寄生虫对于制定预防策略和研究宿主与环境的关系至关重要。本研究利用核 18S rDNA(SSU)序列对从巴维水库捕获的鱼类线虫寄生虫进行了条形编码。核 18S rDNA(SSU)被扩增成两个重叠的扩增片段,并根据与 NCBI GenBank 数据库的序列相似性进行测序以确定物种。本研究的序列(两个片段)与 Eustrongylides 的物种相似度为 98%。本研究样本与 Eustrongylides 物种之间的平均遗传距离值为 0.003。在系统发生树中,该序列也与 Eustrongylides 的物种聚类,且引导值显著。本研究确定从巴维水库捕获的鱼类的线虫寄生虫为 Eustrongylides 种。由于数据库中参考序列不足/缺乏,因此无法进行物种级别的鉴定。这表明在鱼类线虫寄生虫的物种特异性分子标记方面存在知识空白。
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DNA Barcoding of Nematode Parasites Infecting Ompok bimaculatus and Nemacheilus anguilla From Barvi Reservoir, Maharashtra

In the present study, the DNA barcoding of the nematode parasite infecting Ompok bimaculatus and Nemacheilus anguilla fish species was carried out in Barvi Reservoir, Maharashtra. To ascertain the taxonomic status of these nematode parasites, an 18S gene marker was used. Accurate identification of fish parasites is essential to formulate preventive strategies and to study host–environment relations. The present study did barcoding of the nematode parasites of the fishes caught from the Barvi Reservoir using the nuclear 18S rDNA (SSU) sequence. The nuclear 18S rDNA (SSU) was amplified into two overlapping amplicons and sequenced to identify the species based on the sequence similarity with the NCBI GenBank database. The present study sequences (both fragments) showed 98% similarity with the species of Eustrongylides. The average genetic distance value between the present study sample and species of Eustrongylides was 0.003. In the phylogenetic tree also, the sequence was clustered with the species of Eustrongylides with significant bootstrap values. The present study identified the nematode parasite of the fish caught from the Barvi Reservoir, as species of Eustrongylides. The species-level identification could not be possible due to the insufficient/lack of reference sequences in the database. It indicates the knowledge gap concerning the species-specific molecular markers for nematode parasites of the fish.

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来源期刊
Journal of Applied Ichthyology
Journal of Applied Ichthyology 生物-海洋与淡水生物学
CiteScore
2.30
自引率
11.10%
发文量
73
审稿时长
3-8 weeks
期刊介绍: The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.
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