Gowhar Iqbal, Annam Pavan Kumar, Amjad Khansaheb Balange, Sanath Kumar, K. V. Rajendran, Sonal Suman, Nahida Quyoom, Sangeetha S., Showkat Ahmad Dar
{"title":"马哈拉施特拉邦 Barvi 水库中感染 Ompok bimaculatus 和 Nemacheilus anguilla 的线虫寄生虫的 DNA 条形码","authors":"Gowhar Iqbal, Annam Pavan Kumar, Amjad Khansaheb Balange, Sanath Kumar, K. V. Rajendran, Sonal Suman, Nahida Quyoom, Sangeetha S., Showkat Ahmad Dar","doi":"10.1155/2024/9644023","DOIUrl":null,"url":null,"abstract":"<div>\n <p>In the present study, the DNA barcoding of the nematode parasite infecting <i>Ompok bimaculatus</i> and <i>Nemacheilus anguilla</i> fish species was carried out in Barvi Reservoir, Maharashtra. To ascertain the taxonomic status of these nematode parasites, an 18S gene marker was used. Accurate identification of fish parasites is essential to formulate preventive strategies and to study host–environment relations. The present study did barcoding of the nematode parasites of the fishes caught from the Barvi Reservoir using the nuclear 18S rDNA (SSU) sequence. The nuclear 18S rDNA (SSU) was amplified into two overlapping amplicons and sequenced to identify the species based on the sequence similarity with the NCBI GenBank database. The present study sequences (both fragments) showed 98% similarity with the species of Eustrongylides. The average genetic distance value between the present study sample and species of Eustrongylides was 0.003. In the phylogenetic tree also, the sequence was clustered with the species of Eustrongylides with significant bootstrap values. The present study identified the nematode parasite of the fish caught from the Barvi Reservoir, as species of Eustrongylides. The species-level identification could not be possible due to the insufficient/lack of reference sequences in the database. It indicates the knowledge gap concerning the species-specific molecular markers for nematode parasites of the fish.</p>\n </div>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/9644023","citationCount":"0","resultStr":"{\"title\":\"DNA Barcoding of Nematode Parasites Infecting Ompok bimaculatus and Nemacheilus anguilla From Barvi Reservoir, Maharashtra\",\"authors\":\"Gowhar Iqbal, Annam Pavan Kumar, Amjad Khansaheb Balange, Sanath Kumar, K. V. Rajendran, Sonal Suman, Nahida Quyoom, Sangeetha S., Showkat Ahmad Dar\",\"doi\":\"10.1155/2024/9644023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>In the present study, the DNA barcoding of the nematode parasite infecting <i>Ompok bimaculatus</i> and <i>Nemacheilus anguilla</i> fish species was carried out in Barvi Reservoir, Maharashtra. To ascertain the taxonomic status of these nematode parasites, an 18S gene marker was used. Accurate identification of fish parasites is essential to formulate preventive strategies and to study host–environment relations. The present study did barcoding of the nematode parasites of the fishes caught from the Barvi Reservoir using the nuclear 18S rDNA (SSU) sequence. The nuclear 18S rDNA (SSU) was amplified into two overlapping amplicons and sequenced to identify the species based on the sequence similarity with the NCBI GenBank database. The present study sequences (both fragments) showed 98% similarity with the species of Eustrongylides. The average genetic distance value between the present study sample and species of Eustrongylides was 0.003. In the phylogenetic tree also, the sequence was clustered with the species of Eustrongylides with significant bootstrap values. The present study identified the nematode parasite of the fish caught from the Barvi Reservoir, as species of Eustrongylides. The species-level identification could not be possible due to the insufficient/lack of reference sequences in the database. 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DNA Barcoding of Nematode Parasites Infecting Ompok bimaculatus and Nemacheilus anguilla From Barvi Reservoir, Maharashtra
In the present study, the DNA barcoding of the nematode parasite infecting Ompok bimaculatus and Nemacheilus anguilla fish species was carried out in Barvi Reservoir, Maharashtra. To ascertain the taxonomic status of these nematode parasites, an 18S gene marker was used. Accurate identification of fish parasites is essential to formulate preventive strategies and to study host–environment relations. The present study did barcoding of the nematode parasites of the fishes caught from the Barvi Reservoir using the nuclear 18S rDNA (SSU) sequence. The nuclear 18S rDNA (SSU) was amplified into two overlapping amplicons and sequenced to identify the species based on the sequence similarity with the NCBI GenBank database. The present study sequences (both fragments) showed 98% similarity with the species of Eustrongylides. The average genetic distance value between the present study sample and species of Eustrongylides was 0.003. In the phylogenetic tree also, the sequence was clustered with the species of Eustrongylides with significant bootstrap values. The present study identified the nematode parasite of the fish caught from the Barvi Reservoir, as species of Eustrongylides. The species-level identification could not be possible due to the insufficient/lack of reference sequences in the database. It indicates the knowledge gap concerning the species-specific molecular markers for nematode parasites of the fish.
期刊介绍:
The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.