Yoshikazu Sakagami , Katsuhiro Yamasaki , Hiroshima Yokoyama , Youki Ose , Takahiko Sato
{"title":"用液相电泳法检测消毒剂的DNA修复","authors":"Yoshikazu Sakagami , Katsuhiro Yamasaki , Hiroshima Yokoyama , Youki Ose , Takahiko Sato","doi":"10.1016/0167-8817(88)90004-1","DOIUrl":null,"url":null,"abstract":"<div><p>The DNA-damaging capacity and the mutagenicity of 6 disinfectants were studied by liquid rec-assay and Ames test. 5 disinfectants were found to be positive in DNA-damaging capacity while only one of them showed clear mutagenicity in the Ames test. Liquid rec-assay by direct incubation with S9 mix was the most sensitive method and gave the best correlation between the growth ratio (R 50) and the time lag, both of which compared Rec<sup>+</sup> and Rec<sup>−</sup>. liquid rec-assay may be useful for detecting the DNA-damaging capacity of chemicals with a strong killing effect.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90004-1","citationCount":"22","resultStr":"{\"title\":\"DNA repair test of disinfectants by liquid rec-assay\",\"authors\":\"Yoshikazu Sakagami , Katsuhiro Yamasaki , Hiroshima Yokoyama , Youki Ose , Takahiko Sato\",\"doi\":\"10.1016/0167-8817(88)90004-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The DNA-damaging capacity and the mutagenicity of 6 disinfectants were studied by liquid rec-assay and Ames test. 5 disinfectants were found to be positive in DNA-damaging capacity while only one of them showed clear mutagenicity in the Ames test. Liquid rec-assay by direct incubation with S9 mix was the most sensitive method and gave the best correlation between the growth ratio (R 50) and the time lag, both of which compared Rec<sup>+</sup> and Rec<sup>−</sup>. liquid rec-assay may be useful for detecting the DNA-damaging capacity of chemicals with a strong killing effect.</p></div>\",\"PeriodicalId\":100936,\"journal\":{\"name\":\"Mutation Research/DNA Repair Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0167-8817(88)90004-1\",\"citationCount\":\"22\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0167881788900041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0167881788900041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
DNA repair test of disinfectants by liquid rec-assay
The DNA-damaging capacity and the mutagenicity of 6 disinfectants were studied by liquid rec-assay and Ames test. 5 disinfectants were found to be positive in DNA-damaging capacity while only one of them showed clear mutagenicity in the Ames test. Liquid rec-assay by direct incubation with S9 mix was the most sensitive method and gave the best correlation between the growth ratio (R 50) and the time lag, both of which compared Rec+ and Rec−. liquid rec-assay may be useful for detecting the DNA-damaging capacity of chemicals with a strong killing effect.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
