小鼠永久性心包导管技术

IF 8.4 1区医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS JACC: Basic to Translational Science Pub Date : 2024-10-01 DOI:10.1016/j.jacbts.2024.05.015

Vitali Rusinkevich MD, MS, PhD , David Elias BS , Monica Vladut Talor MSc , Daniela Čiháková MD, PhD

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引用次数: 0

摘要

心包内给药为心脏疗法提供了一条途径。小鼠心脏的大小和膜的厚度给导管插入带来了挑战，阻碍了心包靶向治疗。我们的目标是开发一种小鼠心包导管插入手术技术，并评估其是否适合心包内给药，包括在心肌缺血/再灌注模型中的应用。我们在 BALB/cJ 和 ΔdblGATA1 小鼠中成功植入了导管，展示了荧光珠和嗜酸性粒细胞在心包内的输送。我们展示了心包导管植入术与心肌缺血/再灌注模型的结合。可靠的导管技术能在小鼠心包内输送治疗药物，为基础研究和转化研究中研究心包空间和纵隔提供了宝贵的工具。

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The Technique of Permanent Pericardial Catheter in Mice

Intrapericardial delivery offers a route for heart therapies. Mouse heart size and membrane thickness pose catheterization challenges, hampering pericardium-targeted treatments. The objectives were to develop a mouse surgical technique for pericardial catheter insertion and to assess its suitability for intrapericardial delivery, including use with a myocardial ischemia/reperfusion model. We used successful catheter implantation in BALB/cJ and ΔdblGATA1 mice, showcasing intrapericardial delivery with fluorescent beads and eosinophils. We demonstrated a combination of pericardial catheterization with myocardial ischemia/reperfusion model. A reliable catheterization technique, enabling intrapericardial delivery of therapeutic agents in mice provides a valuable tool for studying the pericardial space and mediastinum in basic and translational research.

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来源期刊

JACC: Basic to Translational Science CARDIAC & CARDIOVASCULAR SYSTEMS-

CiteScore

14.20

自引率

1.00%

发文量

161

审稿时长

16 weeks

期刊介绍： JACC: Basic to Translational Science is an open access journal that is part of the renowned Journal of the American College of Cardiology (JACC). It focuses on advancing the field of Translational Cardiovascular Medicine and aims to accelerate the translation of new scientific discoveries into therapies that improve outcomes for patients with or at risk for Cardiovascular Disease. The journal covers thematic areas such as pre-clinical research, clinical trials, personalized medicine, novel drugs, devices, and biologics, proteomics, genomics, and metabolomics, as well as early phase clinical trial methodology.