染色质转录延伸--结构视角。

IF 4.7 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Molecular Biology Pub Date : 2024-10-29 DOI:10.1016/j.jmb.2024.168845
Lucas Farnung
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引用次数: 0

摘要

在真核细胞中,RNA聚合酶II的转录是在染色质背景下进行的,转录机器在穿越基因体时需要穿过核小体。结构生物学的最新进展让人们对转录伸长的内在机制有了前所未有的深入了解。这篇综述从结构的角度阐述了通过染色质进行转录的问题,重点是转录 RNA 聚合酶 II-核小体复合物高分辨率结构的最新发现。我将讨论 RNA 聚合酶 II 如何与 SPT4/5、SPT6、ELOF1 和 PAF1 复合物等延伸因子协同作用,并通过核小体进行转录。这篇综述探讨了随着聚合酶的推进,核糖体 DNA 逐步解开的过程、延伸因子在促进这一过程中的作用,以及转录过程中核糖体保留和转移的机制。这一结构性视角为理解转录机制与染色质之间错综复杂的相互作用奠定了基础,有助于深入了解细胞如何在遗传可及性需求与维持基因组稳定性和表观遗传调控之间取得平衡。
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Chromatin Transcription Elongation - A Structural Perspective.

In eukaryotic cells, transcription by RNA polymerase II occurs in the context of chromatin, requiring the transcription machinery to navigate through nucleosomes as it traverses gene bodies. Recent advances in structural biology have provided unprecedented insights into the mechanisms underlying transcription elongation. This review presents a structural perspective on transcription through chromatin, focusing on the latest findings from high-resolution structures of transcribing RNA polymerase II-nucleosome complexes. I discuss how RNA polymerase II, in concert with elongation factors such as SPT4/5, SPT6, ELOF1, and the PAF1 complex, engages with and transcribes through nucleosomes. The review examines the stepwise unwrapping of nucleosomal DNA as polymerase advances, the roles of elongation factors in facilitating this process, and the mechanisms of nucleosome retention and transfer during transcription. This structural perspective provides a foundation for understanding the intricate interplay between the transcription machinery and chromatin, offering insights into how cells balance the need for genetic accessibility with the maintenance of genome stability and epigenetic regulation.

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来源期刊
Journal of Molecular Biology
Journal of Molecular Biology 生物-生化与分子生物学
CiteScore
11.30
自引率
1.80%
发文量
412
审稿时长
28 days
期刊介绍: Journal of Molecular Biology (JMB) provides high quality, comprehensive and broad coverage in all areas of molecular biology. The journal publishes original scientific research papers that provide mechanistic and functional insights and report a significant advance to the field. The journal encourages the submission of multidisciplinary studies that use complementary experimental and computational approaches to address challenging biological questions. Research areas include but are not limited to: Biomolecular interactions, signaling networks, systems biology; Cell cycle, cell growth, cell differentiation; Cell death, autophagy; Cell signaling and regulation; Chemical biology; Computational biology, in combination with experimental studies; DNA replication, repair, and recombination; Development, regenerative biology, mechanistic and functional studies of stem cells; Epigenetics, chromatin structure and function; Gene expression; Membrane processes, cell surface proteins and cell-cell interactions; Methodological advances, both experimental and theoretical, including databases; Microbiology, virology, and interactions with the host or environment; Microbiota mechanistic and functional studies; Nuclear organization; Post-translational modifications, proteomics; Processing and function of biologically important macromolecules and complexes; Molecular basis of disease; RNA processing, structure and functions of non-coding RNAs, transcription; Sorting, spatiotemporal organization, trafficking; Structural biology; Synthetic biology; Translation, protein folding, chaperones, protein degradation and quality control.
期刊最新文献
Editorial Board Outside Front Cover Assembly of the human multi-tRNA synthetase complex through leucine zipper motifs. Corrigendum to “The Role of ATG9 Vesicles in Autophagosome Biogenesis” [J. Mol. Biol. 436(15) (2024) 168489] Structural studies on Mycobacterial NudC reveal a class of zinc independent NADH pyrophosphatase.
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