妊娠期前三个月和后三个月妊娠糖尿病患者血浆总外泌体和胎盘衍生外泌体的蛋白质组学分析。

IF 2.8 2区 医学 Q1 OBSTETRICS & GYNECOLOGY BMC Pregnancy and Childbirth Pub Date : 2024-10-30 DOI:10.1186/s12884-024-06919-9
Jing Lin, Danqing Zhao, Yi Liang, Zhiyuan Liang, Mingxian Wang, Xiaoxiao Tang, Hongbin Zhuang, Hanghang Wang, Xiaoping Yin, Yuhan Huang, Li Yin, Liming Shen
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引用次数: 0

摘要

妊娠糖尿病(GDM)是妊娠期间首次出现的自发性高血糖。早期诊断和干预对疾病的治疗非常重要。本研究比较分析了妊娠12-16周的GDM孕妇、妊娠24-28周的GDM患者及其对照组的总血浆外泌体(T-EXO)和胎盘洐生外泌体(PLAP-EXO)的蛋白质,以研究与外泌体相关的GDM发病机制和生物标志物。通过蛋白质组学方法提取并研究了外泌体蛋白,然后应用生物信息学分析了各组间的差异表达蛋白(DEPs)。在妊娠12-16周和24-28周时,在T-EXO中分别发现了36个和21个差异表达蛋白,而在PLAP-EXO中分别发现了34个和20个差异表达蛋白。这些蛋白质主要参与补体途径、免疫、炎症、凝血和其他途径,其中大多数以前曾被报道为与GDM相关的血液或外泌体蛋白质。研究结果表明,GDM 的发展是一个渐进的过程,早期的变化会促进疾病的发展。母体和胎盘因素在 GDM 的发病机制中起着关键作用。这些蛋白质尤其是 Hub 蛋白有可能成为预测和诊断 GDM 的生物标志物。
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Proteomic analysis of plasma total exosomes and placenta-derived exosomes in patients with gestational diabetes mellitus in the first and second trimesters.

Gestational diabetes mellitus (GDM) is the first spontaneous hyperglycemia during pregnancy. Early diagnosis and intervention are important for the management of the disease. This study compared and analyzed the proteins of total plasma exosomes (T-EXO) and placental-derived exosomes (PLAP-EXO) in pregnant women who subsequently developed GDM (12-16 weeks), GDM patients (24-28 weeks) and their corresponding controls to investigate the pathogenesis and biomarkers of GDM associated with exosomes. The exosomal proteins were extracted and studied by proteomics approach, then bioinformatics analysis was applied to the differentially expressed proteins (DEPs) between the groups. At 12-16 and 24-28 weeks of gestation, 36 and 21 DEPs were identified in T-EXO, while 34 and 20 DEPs were identified in PLAP-EXO between GDM and controls, respectively. These proteins are mainly involved in complement pathways, immunity, inflammation, coagulation and other pathways, most of them have been previously reported as blood or exosomal proteins associated with GDM. The findings suggest that the development of GDM is a progressive process and that early changes promote the development of the disease. Maternal and placental factors play a key role in the pathogenesis of GDM. These proteins especially Hub proteins have the potential to become predictive and diagnostic biomarkers for GDM.

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来源期刊
BMC Pregnancy and Childbirth
BMC Pregnancy and Childbirth OBSTETRICS & GYNECOLOGY-
CiteScore
4.90
自引率
6.50%
发文量
845
审稿时长
3-8 weeks
期刊介绍: BMC Pregnancy & Childbirth is an open access, peer-reviewed journal that considers articles on all aspects of pregnancy and childbirth. The journal welcomes submissions on the biomedical aspects of pregnancy, breastfeeding, labor, maternal health, maternity care, trends and sociological aspects of pregnancy and childbirth.
期刊最新文献
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