中国松树植物色素相互作用因子(PIF)家族的全基因组分析及其在光和赤霉素信号传导中的潜在作用

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-10-30 DOI:10.1186/s12864-024-10915-w
Yingtian Guo, Chengyan Deng, Guizhi Feng, Dan Liu
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引用次数: 0

摘要

植物色素相互作用因子(PIFs)是基本螺旋环螺旋(bHLH)家族中的一个转录因子亚群,在整合各种环境信号以调控植物生长和发育方面发挥着至关重要的作用。尽管 PIFs 在这些过程中意义重大,但目前尚未对针叶树中的 PIFs 进行全面的全基因组分析。本研究在中国松树中发现了三个 PtPIF 基因,并将其分为三个亚组,其中 PtPIF1 和 PtPIF3 蛋白的保守基序表明存在 APA/APB 基序和 bHLH 结构域。系统进化分析表明,PtPIF1 和 PtPIF3 蛋白分别属于 PIF7/8 和 PIF3 组,在裸子植物中相对保守。此外,在针叶树中还发现了一类缺乏 APA/APB 基序的 PIF,表明其功能可能与传统的 PIF 不同。对 PtPIF 基因的顺式元件进行了系统研究,并分析了 PtPIF 基因在不同组织以及不同光照、温度和植物激素条件下的表达情况,结果表明 PtPIF1 和 PtPIF3 具有相似的表达谱。对蛋白-蛋白相互作用和共表达网络的研究推测,PtPIF 和 PtPHYA/Bs 参与了昼夜节律和激素信号转导。对转录组数据的进一步分析和实验验证表明,PtPIF3 和 PtPHYB1 之间存在相互作用,可能与昼夜节律有关。值得注意的是,该研究发现 PtPIF3 可能通过与 PtDELLAs 的相互作用参与赤霉素(GA)信号转导,这表明 PtPIF3 在介导光和 GA 响应方面具有潜在作用。总之,这项研究为今后研究 PIFs 在针叶树生长和发育中的功能奠定了基础。
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Genome-wide analysis of phytochrome-interacting factor (PIF) families and their potential roles in light and gibberellin signaling in Chinese pine.

Phytochrome-interacting factors (PIFs) are a subgroup of transcription factors within the basic helix-loop-helix (bHLH) family, playing a crucial role in integrating various environmental signals to regulate plant growth and development. Despite the significance of PIFs in these processes, a comprehensive genome-wide analysis of PIFs in conifers has yet to be conducted. In this investigation, three PtPIF genes were identified in Chinese pine, categorized into three subgroups, with conserved motifs indicating the presence of the APA/APB motif and bHLH domain in the PtPIF1 and PtPIF3 proteins. Phylogenetic analysis revealed that the PtPIF1 and PtPIF3 proteins belong to the PIF7/8 and PIF3 groups, respectively, and were relatively conserved among gymnosperms. Additionally, a class of PIF lacking APA/APB motif was identified in conifers, suggesting its function may differ from that of traditional PIFs. The cis-elements of the PtPIF genes were systematically examined, and analysis of PtPIF gene expression across various tissues and under different light, temperature, and plant hormone conditions demonstrated similar expression profiles for PtPIF1 and PtPIF3. Investigations into protein-protein interactions and co-expression networks speculated the involvement of PtPIFs and PtPHYA/Bs in circadian rhythms and hormone signal transduction. Further analysis of transcriptome data and experimental validation indicated an interaction between PtPIF3 and PtPHYB1, potentially linked to diurnal rhythms. Notably, the study revealed that PtPIF3 may be involved in gibberellic acid (GA) signaling through its interaction with PtDELLAs, suggesting a potential role for PtPIF3 in mediating both light and GA responses. Overall, this research provides a foundation for future studies investigating the functions of PIFs in conifer growth and development.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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