Lukasz Antoniewicz, Georgy Melnikov, Gustaf Lyytinen, Anders Blomberg, Jenny A Bosson, Linnea Hedman, Fariborz Mobarrez, Magnus Lundbäck
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引用次数: 0
摘要
加热烟草制品(HTPs)的出现为尼古丁给药系统及其健康影响的研究引入了新的变量。鉴于人们对心血管影响的担忧,本研究旨在阐明吸入加热烟草制品对年轻健康人细胞外小泡(EV)水平的急性影响。在这项受控急性暴露研究中,23 名年轻健康的志愿者吸入了 HTP。在接触前后,通过流式细胞术对内皮和血小板来源的 EV 水平进行了量化。数据分析采用多元方差分析来评估 EV 浓度的变化。我们的研究结果表明,短期吸入尼古丁的 HTP 后,内皮和血小板来源的 EV 明显增加。值得注意的是,白细胞和中性粒细胞来源的 EVs 没有明显变化。EVs 的增加表明存在急性血管应激,在暴露后 4 小时达到峰值。内皮和血小板衍生 EVs 的增加与文献记载的对急性血管损伤的反应一致,与传统香烟和电子烟的影响相似。尽管 HTP 在市场上被当作更安全的替代品,但我们的研究结果表明,含有尼古丁的 HTP 仍可能对血管构成重大风险。这些发现为越来越多的证据提供了依据,告诫人们不要认为 HTPs 是安全的,并强化了针对尼古丁递送技术的监管监督和公共卫生措施的重要性。试验注册:ClinicalTrials.gov ID:NCT04824495,注册日期为 2021-01-07。
Vascular Stress Markers Following Inhalation of Heated Tobacco Products: A Study on Extracellular Vesicles.
The advent of heated tobacco products (HTPs) has introduced new variables in the study of nicotine delivery systems and their health implications. Amidst concerns over cardiovascular effects, this study aims to elucidate the acute impact of HTP inhalation on extracellular vesicles (EV) levels in young, healthy individuals. In this controlled, acute exposure study, 23 young, healthy volunteers were subjected to HTP inhalation. EV levels of endothelial and platelet origin were quantified through flow cytometry before and after exposure. Data analysis was performed using multiple measures ANOVA to assess changes in EV concentrations. Our findings reveal a significant increase in EVs of endothelial and platelet origin following short-term HTP inhalation with nicotine. Notably, no significant change was observed in leukocyte- and neutrophil-derived EVs. This increase in EVs suggests acute vascular stress, with peak levels observed 4 h post-exposure. The rise in endothelial and platelet-derived EVs aligns with documented responses to acute vascular injury, paralleling the effects seen with traditional cigarette and e-cigarette use. Despite HTPs being marketed as safer alternatives, our results indicate that nicotine-containing HTPs may still pose significant vascular risks. These findings contribute to the growing body of evidence cautioning against the perceived safety of HTPs and reinforce the importance of regulatory oversight and public health initiatives targeting nicotine delivery technologies. Trial Registrations: ClinicalTrials.gov ID: NCT04824495, registered 2021-01-07.
期刊介绍:
Cardiovascular Toxicology is the only journal dedicated to publishing contemporary issues, timely reviews, and experimental and clinical data on toxicological aspects of cardiovascular disease. CT publishes papers that will elucidate the effects, molecular mechanisms, and signaling pathways of environmental toxicants on the cardiovascular system. Also covered are the detrimental effects of new cardiovascular drugs, and cardiovascular effects of non-cardiovascular drugs, anti-cancer chemotherapy, and gene therapy. In addition, Cardiovascular Toxicology reports safety and toxicological data on new cardiovascular and non-cardiovascular drugs.