从白纹伊蚊中提取的 Elizabethkingia anophelis 产品对 RAW264 鼠巨噬细胞系的趋化活性。

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2024-10-31 DOI:10.7883/yoken.JJID.2024.227
Satoru Yui, Norihiko Fujii, Jo Terauchi, Nana Tanabe, Marie Kanno, Kouta Umehara, Ryosuke Iijima, Riyo Kamata, Naoki Ohkura, Seishi Kishimoto, Toshinori Sasaki
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引用次数: 0

摘要

登革热病毒会进入皮肤巨噬细胞,而巨噬细胞来自蚊子叮咬后的其他组织。我们研究了登革热病媒蚊子白纹伊蚊对 RAW264 小鼠巨噬细胞系的趋化因子。我们发现,从蚊子体内分离出来的伊丽莎白金丝楠对 RAW264 细胞具有迁移诱导活性。使用乙酸乙酯提取活性物质以诱导趋化运动。使用反相色谱法在多个馏分中都能洗脱出趋化活性,这表明该活性是由多种物质引起的。我们从日本东京都富山公园采集的野生白纹伊蚊中分离出三个细菌菌落。细菌的 16S rRNA 基因序列与 Lonsdalea quercina 最为相似。这些细菌对 RAW264 细胞也具有迁移诱导活性。蚊子细菌的迁移诱导活性可能是蚊子介导的病毒感染的一个新方面。
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Chemotactic activity of products of Elizabethkingia anophelis derived from Aedes albopictus against RAW264 murine macrophage cell line.

Dengue viruses enter dermal macrophages which are derived from other tissues after mosquito bites. We examined chemotactic factors derived from the dengue vector mosquito, Aedes albopictus, toward a RAW264 murine macrophage cell line. We found that Elizabethkingia anophelis that was isolated from mosquitoes exhibited migration-inducing activity toward RAW264 cells. The active substances were extracted using ethyl acetate to induce chemotactic movements. Chemotactic activity was eluted in the several fractions using the reversed-phase chromatography, suggesting that multiple substances were responsible for the activity. We isolated three bacterial colonies from the wild A. albopictus mosquitoes collected in Toyama Park, Tokyo prefecture, Japan. The bacterial 16S rRNA gene sequences were the most similar to those of Lonsdalea quercina. These bacteria also exhibited migration-inducing activity toward RAW264 cells. The migration-inducing activity of mosquito bacteria might be a new aspect of mosquito-mediated viral infections.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
期刊最新文献
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