用 MPXV A29 和 A35 抗体对猴痘皮肤病变进行免疫组化分析:临床病理形式的新见解。

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2024-10-31 DOI:10.7883/yoken.JJID.2024.145
Mei Shi, Chun-Yu Zhang, Dan-Yang Zou, Juan Wu, Nan-Hui Wu, Li-Yan Ni, Zhi-Fang Guan, Qian Du, Rui-Rui Peng, Fu-Quan Long
{"title":"用 MPXV A29 和 A35 抗体对猴痘皮肤病变进行免疫组化分析:临床病理形式的新见解。","authors":"Mei Shi, Chun-Yu Zhang, Dan-Yang Zou, Juan Wu, Nan-Hui Wu, Li-Yan Ni, Zhi-Fang Guan, Qian Du, Rui-Rui Peng, Fu-Quan Long","doi":"10.7883/yoken.JJID.2024.145","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study was to describe the clinical, histopathological, and immunohistochemical characteristics of MPX and offer meaningful insights into the clinicopathology of MPX. We recruited eight men who had sex with men diagnosed with MPX based on positive results from MPX Virus (MPXV)-specific polymerase chain reaction. Skin biopsies were obtained from four selected lesions, including typical and atypical lesions. Histopathological examinations of atypical solitary ulceration revealed infiltrating inflammatory cells predominantly composed of plasma cells and lymphocytes, forming a \"sleeve\" around the superficial vessels of the dermis. These features might be misinterpreted as indicative of cutaneous syphilis infection. Meanwhile, typical pustular lesions displayed central necrotic epidermis accompanied by perivascular inflammatory infiltrate dominated by neutrophils, as well as ballooning and reticular degeneration of keratinocytes. Additionally, multinucleated keratinocytes and eosinophilic cytoplasmic inclusions known as Guarnieri's bodies were also observed. Importantly, this study represented the pioneering report on immunohistochemical detection of MPXV A29 and A35 proteins in skin lesions, distinguishing it from previous studies that focused on detecting vaccinia virus protein. The anti-MPXV A29 antibody exhibited robust cytoplasmic staining specifically within affected keratinocytes in both adjacent epidermis and hair follicles, thereby it could contribute to the diagnosis of MPX, especially for cases with atypical skin lesions.</p>","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical Analyses of Monkeypox Skin Lesions with MPXV A29 and A35 Antibodies: A Novel Insight for Clinical-Histopathological Forms.\",\"authors\":\"Mei Shi, Chun-Yu Zhang, Dan-Yang Zou, Juan Wu, Nan-Hui Wu, Li-Yan Ni, Zhi-Fang Guan, Qian Du, Rui-Rui Peng, Fu-Quan Long\",\"doi\":\"10.7883/yoken.JJID.2024.145\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of this study was to describe the clinical, histopathological, and immunohistochemical characteristics of MPX and offer meaningful insights into the clinicopathology of MPX. We recruited eight men who had sex with men diagnosed with MPX based on positive results from MPX Virus (MPXV)-specific polymerase chain reaction. Skin biopsies were obtained from four selected lesions, including typical and atypical lesions. Histopathological examinations of atypical solitary ulceration revealed infiltrating inflammatory cells predominantly composed of plasma cells and lymphocytes, forming a \\\"sleeve\\\" around the superficial vessels of the dermis. These features might be misinterpreted as indicative of cutaneous syphilis infection. Meanwhile, typical pustular lesions displayed central necrotic epidermis accompanied by perivascular inflammatory infiltrate dominated by neutrophils, as well as ballooning and reticular degeneration of keratinocytes. Additionally, multinucleated keratinocytes and eosinophilic cytoplasmic inclusions known as Guarnieri's bodies were also observed. Importantly, this study represented the pioneering report on immunohistochemical detection of MPXV A29 and A35 proteins in skin lesions, distinguishing it from previous studies that focused on detecting vaccinia virus protein. The anti-MPXV A29 antibody exhibited robust cytoplasmic staining specifically within affected keratinocytes in both adjacent epidermis and hair follicles, thereby it could contribute to the diagnosis of MPX, especially for cases with atypical skin lesions.</p>\",\"PeriodicalId\":14608,\"journal\":{\"name\":\"Japanese journal of infectious diseases\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese journal of infectious diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.7883/yoken.JJID.2024.145\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of infectious diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.7883/yoken.JJID.2024.145","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在描述 MPX 的临床、组织病理学和免疫组化特征,为 MPX 的临床病理学提供有意义的见解。我们招募了八名根据 MPX 病毒(MPXV)特异性聚合酶链反应阳性结果被诊断为 MPX 的男男性行为者。我们选取了四个皮损部位进行皮肤活检,包括典型皮损和非典型皮损。非典型单发溃疡的组织病理学检查显示,浸润性炎症细胞主要由浆细胞和淋巴细胞组成,在真皮浅层血管周围形成一个 "套筒"。这些特征可能会被误认为是皮肤梅毒感染的表现。与此同时,典型的脓疱性病变表现为表皮中央坏死,伴有以中性粒细胞为主的血管周围炎症浸润,以及角质细胞气球化和网状变性。此外,还观察到多核角质细胞和嗜酸性细胞质包涵体(称为瓜尔尼里体)。重要的是,这项研究开创了免疫组化法检测皮肤病变中 MPXV A29 和 A35 蛋白的先河,有别于以往侧重于检测疫苗病毒蛋白的研究。抗 MPXV A29 抗体在邻近表皮和毛囊中受影响的角质细胞内均表现出特异性的强细胞质染色,因此有助于 MPX 的诊断,尤其是对非典型皮损病例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Immunohistochemical Analyses of Monkeypox Skin Lesions with MPXV A29 and A35 Antibodies: A Novel Insight for Clinical-Histopathological Forms.

The aim of this study was to describe the clinical, histopathological, and immunohistochemical characteristics of MPX and offer meaningful insights into the clinicopathology of MPX. We recruited eight men who had sex with men diagnosed with MPX based on positive results from MPX Virus (MPXV)-specific polymerase chain reaction. Skin biopsies were obtained from four selected lesions, including typical and atypical lesions. Histopathological examinations of atypical solitary ulceration revealed infiltrating inflammatory cells predominantly composed of plasma cells and lymphocytes, forming a "sleeve" around the superficial vessels of the dermis. These features might be misinterpreted as indicative of cutaneous syphilis infection. Meanwhile, typical pustular lesions displayed central necrotic epidermis accompanied by perivascular inflammatory infiltrate dominated by neutrophils, as well as ballooning and reticular degeneration of keratinocytes. Additionally, multinucleated keratinocytes and eosinophilic cytoplasmic inclusions known as Guarnieri's bodies were also observed. Importantly, this study represented the pioneering report on immunohistochemical detection of MPXV A29 and A35 proteins in skin lesions, distinguishing it from previous studies that focused on detecting vaccinia virus protein. The anti-MPXV A29 antibody exhibited robust cytoplasmic staining specifically within affected keratinocytes in both adjacent epidermis and hair follicles, thereby it could contribute to the diagnosis of MPX, especially for cases with atypical skin lesions.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
期刊最新文献
Streptococcal Toxic Shock Syndrome Caused by a Streptococcus pyogenes emm22 Genotype with a CsrS Mutation: a Case Report. A Case of Food Poisoning Caused by Campylobacter jejuni after the Ingestion of Undercooked Chicken Meal with Subsequent Development of Guillain-Barré Syndrome. An Analysis of Factors Contributing to Household Transmission of COVID-19 Using Data from Active Epidemiological Investigations Performed in the Setagaya Ward of Tokyo, Japan. Characterization of the Proinflammatory Cytokine Profile during Acute SARS-CoV-2 Infection in People with Human Immunodeficiency Virus. Distribution and Antimicrobial Susceptibility Pattern of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated in Chubu Region, Japan.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1