无质粒大肠杆菌通过添加丙酮酸钠结合葡萄糖和温度控制策略提高 D-泛酸的生物合成。

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-11-04 DOI:10.1093/jambio/lxae267
Hai-Yan Zhou, Jin-Bang Peng, Yi-Hong Chen, Zi-Jian Yang, Zhi-Qiang Liu, Yu-Guo Zheng
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引用次数: 0

摘要

目的:D-泛酸(D-PA)是一种重要的维生素,广泛应用于饲料、制药和食品行业。本研究旨在提高重组大肠杆菌的 D-PA 产量,无需质粒和诱导剂诱导:方法和结果:通过优化摇瓶发酵培养基,D-PA 滴度提高了 39.50%(3.32 g L-1)。随后,对 5 升发酵罐中的饲料批量发酵进行了具体研究。首先,两阶段温度控制策略使 D-PA 滴度达到 52.09 g L-1。此外,还提出了两阶段葡萄糖喂料,D-PA 滴度提高到 65.29 g L-1。研究还发现,适量的丙酮酸钠有利于细胞生长和 D-PA 合成。最后,两阶段葡萄糖喂养结合丙酮酸钠添加,D-PA 产量大幅提高,滴度达到 72.90 g L-1:通过本研究建立的发酵工艺,即添加丙酮酸钠与温度和葡萄糖控制策略相结合,D-PA 的合成得到了显著改善。本研究的结果可为 D-PA 的工业发酵生产提供重要参考。
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Enhanced d-pantothenic acid biosynthesis by plasmid-free Escherichia coli through sodium pyruvate addition combined with glucose and temperature control strategy.

Aims: d-pantothenic acid (d-PA) is an important vitamin widely used in the feed, pharmaceutical, and food industries. This study aims to enhance the d-PA production of a recombinant Escherichia coli without plasmid and inducer induction.

Methods and results: The fermentation medium in shake flask was optimized, resulting in a 39.50% increased d-PA titer (3.32 g l-1). Subsequently, the fed-batch fermentation in a 5-l fermenter was specifically investigated. First, a two-stage temperature control strategy led to a d-PA titer of 52.09 g l-1. Additionally, a two-stage glucose feeding was proposed and d-PA titer was increased to 65.29 g l-1. It was also found that an appropriate amount of sodium pyruvate was beneficial to cell growth and d-PA synthesis. Finally, a two-stage glucose feeding combined with sodium pyruvate addition resulted in a substantially improved d-PA production with a titer of 72.90 g l-1.

Conclusion: The d-PA synthesis was significantly improved through the fermentation process established in this work, i.e. sodium pyruvate addition combined with the temperature and glucose control strategy. The results of this study could provide significant reference for the industrial fermentation production of d-PA.

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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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