一种含 DUF3844 结构域的蛋白质是酿酒酵母菌液泡蛋白分选所必需的。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-10-31 DOI:10.2323/jgam.2024.10.001
Tomoaki Inagawa, Kazuma Ohkubo, Masahiro Watanabe, Tomotake Morita, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
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引用次数: 0

摘要

在植物和真菌中,蛋白质向液泡、在动物中向溶酶体的运输对维持细胞平衡至关重要。在酿酒酵母(Saccharomyces cerevisiae)中,以液泡羧肽酶 Y(CPY)为模型对液泡蛋白分拣(VPS)途径进行了深入研究,并发现了许多 VPS 基因。相比之下,人们对酿酒酵母菌(Schizosaccharomyces pombe)的液泡蛋白运输途径仍然知之甚少。在这项研究中,我们发现了一个新的VPS基因(SPBC1709.03),该基因在真菌中广泛保守,但在酿酒酵母中并不保守。由于其 DUF3844 结构域的功能未知,该基因被命名为 vps3844。vps3844 的中断突变体在 CPY 分选和 FM4-64 染料掺入液泡膜方面都存在缺陷。对 Vps3844 蛋白的部分缺失分析表明,在 DUF3844 结构域中,由 354 至 380 个氨基酸组成的区域对蛋白质向液泡的运输非常重要。我们的研究结果首次报道了参与液泡转运的 VPS 基因,该基因在真菌(尤其是球囊霉)中是保守的,但在酿酒葡萄孢中却缺乏代表性。
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A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.

Protein trafficking to vacuoles in plants and fungi, and to lysosomes in animals, is essential for the maintenance of cellular homeostasis. In Saccharomyces cerevisiae, the vacuolar protein sorting (VPS) pathway has been well studied by using vacuolar carboxypeptidase Y (CPY) as a model, and many VPS genes have been identified. By contrast, the vacuolar protein trafficking pathway in Schizosaccharomyces pombe remains poorly understood. In this study, we identified a novel VPS gene (SPBC1709.03) in S. pombe that is broadly conserved in fungi, but not in S. cerevisiae. Owing to its DUF3844 domain of unknown function, the gene was named vps3844. Disruption mutants of vps3844 had defects in both CPY sorting and incorporation of FM4-64 dye into the vacuolar membrane. Partial deletion analysis of the Vps3844 protein revealed that, within the DUF3844 domain, the region comprising amino acids 354 to 380 is important for protein trafficking to the vacuole. Our findings represent the first report of a VPS gene involved in vacuolar transport that is conserved in fungi, particularly S. pombe, but lacks representation in S. cerevisiae.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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