含 3-O-β-D-Glucopyranosyl Platycosides 的生物转化桔梗根提取物在 LPS 刺激的肺泡巨噬细胞 NR8383 细胞中的抗炎活性

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-10-31 DOI:10.4014/jmb.2408.08005
Jeong Won Choi, Hyeok Jin Choi, Chae Sun Na, Hwan Lee, Byung Joo Lee, Kyung-Chul Shin, Jin Boo Jeong
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引用次数: 0

摘要

急性肺损伤(ALI)是一种严重的炎症,其特点是过度的免疫反应和氧化应激,从而导致严重的组织损伤。鉴于对新型治疗药物的需求,本研究旨在探索生物转化桔梗根提取物(BT-PGR)的抗炎活性和机制。目的是评估 BT-PGR 作为 ALI 天然疗法的潜力。BT-PGR 能有效抑制 LPS 诱导的 NR8383 细胞中 NO、iNOS、IL-1β、IL-6 和 TNF-α 的产生。BT-PGR 可抑制 LPS 刺激的 NR8383 细胞中 ERK1/2、p38、JNK 和 p65 的磷酸化。此外,BT-PGR 还抑制了 LPS 介导的 NFκB 荧光素酶活性的激活。BT-PGR 增加了 HO-1 的水平,而 ZnPP 对 HO-1 的抑制减弱了 BT-PGR 介导的对 NO 生成的抑制。此外,LY294002 对 PI3K 的抑制也阻断了 BT-PGR 介导的 HO-1 水平的升高。BT-PGR 增加了核 Nrf2 的水平,用 siRNA 敲除 Nrf2 抑制了 BT-PGR 介导的 HO-1 水平的增加。此外,LY294002 对 PI3K 的抑制也抑制了核 Nrf2 水平的升高。基于这些结果,可以推断 BT-PGR 在大鼠肺泡巨噬细胞中具有抗炎活性,这表明它有可能成为改善 ALI 的天然候选药物。
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Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3-O-β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells.

Acute lung injury (ALI) is a severe inflammatory condition characterized by excessive immune responses and oxidative stress, leading to significant tissue damage. Given the need for novel therapeutic agents, this study aimed to explore the anti-inflammatory activity and mechanisms of biotransformed Platycodon grandiflorum root extracts (BT-PGR), which were enzymatically processed using rapidsase PL Classic from Aspergillus niger. The goal was to assess the potential of BT-PGR as a natural treatment for ALI. BT-PGR effectively inhibited the production of NO, iNOS, IL-1β, IL-6, and TNF-α induced by LPS in NR8383 cells. BT-PGR inhibited the phosphorylation of ERK1/2, p38, JNK and p65 in LPS-stimulated NR8383 cells. In addition, BT-PGR suppressed LPS-mediated activation of NFκB luciferase activity. BT-PGR increased the levels of HO-1 and the inhibition of HO-1 by ZnPP attenuated BT-PGR-mediated inhibition of NO production. In addition, the inhibition of PI3K by LY294002 blocked the BT-PGR-mediated increase of HO-1 level. BT-PGR increased nuclear Nrf2 level and the knockdown of Nrf2 by siRNA inhibited BT-PGR-mediated increase of HO-1 level. In addition, inhibition of PI3K by LY294002 suppressed the increase of nuclear Nrf2 level. Based on these results, it can be inferred that BT-PGR exhibits anti-inflammatory activity in rat alveolar macrophages, suggesting its potential as a natural candidate for the improvement of ALI.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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