剥夺睡眠通过激活 AMPK 途径刺激小鼠的适应性产热。

Tian-Shu Zheng, Xin-Ran Gao, Rui-Ping Xu, Yi-Fei Zhao, Zhi-Teng Yang, De-Hua Wang
{"title":"剥夺睡眠通过激活 AMPK 途径刺激小鼠的适应性产热。","authors":"Tian-Shu Zheng, Xin-Ran Gao, Rui-Ping Xu, Yi-Fei Zhao, Zhi-Teng Yang, De-Hua Wang","doi":"10.1007/s00360-024-01590-0","DOIUrl":null,"url":null,"abstract":"<p><p>Sleep deprivation (SD) can affect the adaptive thermogenesis in laboratory rodents, but the molecular mechanism and the crosstalk with other organs remain largely unknown. In order to investigate the effects and mechanisms of SD on thermoregulation and energy metabolism, here we measured the changes of body weight, body fat mass, body temperature, resting metabolic rate (RMR), and thermogenic gene expression in brown adipose tissue (BAT), white adipose tissue (WAT), skeleton muscle and liver in C57BL/6J mice during 7-day SD with rotating rod sleep deprivation device. Results showed that compared with the control group, the body weight and body fat mass of SD mice were decreased and RMR of SD mice increased. The gene expression of Ampk, Pgc1α and Ucp1 which related to thermogenesis in BAT and WAT were significantly increased, and the expression of Ampk, Serca1, Serca2 and Ucp3 which related to thermogenesis in skeletal muscle were significantly increased in SD mice. Taken together, these data demonstrated that 7-day SD enhanced the adaptive thermogenesis in mice by activating AMPK, including the upregulation of the AMPK - PGC1α - UCP1 pathway in BAT, and the AMPK - UCP3 and SLN - SERCA pathway in skeleton muscle. Our data provide the molecular evidence for SD-stimulated adaptive thermogenesis and energy metabolism in small mammals.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sleep deprivation stimulates adaptive thermogenesis by activating AMPK pathway in mice.\",\"authors\":\"Tian-Shu Zheng, Xin-Ran Gao, Rui-Ping Xu, Yi-Fei Zhao, Zhi-Teng Yang, De-Hua Wang\",\"doi\":\"10.1007/s00360-024-01590-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sleep deprivation (SD) can affect the adaptive thermogenesis in laboratory rodents, but the molecular mechanism and the crosstalk with other organs remain largely unknown. In order to investigate the effects and mechanisms of SD on thermoregulation and energy metabolism, here we measured the changes of body weight, body fat mass, body temperature, resting metabolic rate (RMR), and thermogenic gene expression in brown adipose tissue (BAT), white adipose tissue (WAT), skeleton muscle and liver in C57BL/6J mice during 7-day SD with rotating rod sleep deprivation device. Results showed that compared with the control group, the body weight and body fat mass of SD mice were decreased and RMR of SD mice increased. The gene expression of Ampk, Pgc1α and Ucp1 which related to thermogenesis in BAT and WAT were significantly increased, and the expression of Ampk, Serca1, Serca2 and Ucp3 which related to thermogenesis in skeletal muscle were significantly increased in SD mice. Taken together, these data demonstrated that 7-day SD enhanced the adaptive thermogenesis in mice by activating AMPK, including the upregulation of the AMPK - PGC1α - UCP1 pathway in BAT, and the AMPK - UCP3 and SLN - SERCA pathway in skeleton muscle. Our data provide the molecular evidence for SD-stimulated adaptive thermogenesis and energy metabolism in small mammals.</p>\",\"PeriodicalId\":56033,\"journal\":{\"name\":\"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00360-024-01590-0\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00360-024-01590-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

睡眠剥夺(SD)会影响实验室啮齿动物的适应性产热,但其分子机制以及与其他器官的相互关系仍不清楚。为了研究睡眠剥夺对体温调节和能量代谢的影响及其机制,我们利用旋转棒睡眠剥夺装置测定了C57BL/6J小鼠在7天睡眠剥夺期间体重、体脂量、体温、静息代谢率(RMR)以及棕色脂肪组织(BAT)、白色脂肪组织(WAT)、骨骼肌和肝脏产热基因表达的变化。结果表明,与对照组相比,SD小鼠的体重和体脂肪量下降,RMR增加。SD小鼠BAT和WAT中与产热相关的Ampk、Pgc1α和Ucp1基因表达明显增加,骨骼肌中与产热相关的Ampk、Serca1、Serca2和Ucp3基因表达明显增加。总之,这些数据表明,7 天 SD 可通过激活 AMPK 增强小鼠的适应性产热,包括上调 BAT 中的 AMPK - PGC1α - UCP1 通路,以及骨骼肌中的 AMPK - UCP3 和 SLN - SERCA 通路。我们的数据为SD刺激小型哺乳动物的适应性产热和能量代谢提供了分子证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Sleep deprivation stimulates adaptive thermogenesis by activating AMPK pathway in mice.

Sleep deprivation (SD) can affect the adaptive thermogenesis in laboratory rodents, but the molecular mechanism and the crosstalk with other organs remain largely unknown. In order to investigate the effects and mechanisms of SD on thermoregulation and energy metabolism, here we measured the changes of body weight, body fat mass, body temperature, resting metabolic rate (RMR), and thermogenic gene expression in brown adipose tissue (BAT), white adipose tissue (WAT), skeleton muscle and liver in C57BL/6J mice during 7-day SD with rotating rod sleep deprivation device. Results showed that compared with the control group, the body weight and body fat mass of SD mice were decreased and RMR of SD mice increased. The gene expression of Ampk, Pgc1α and Ucp1 which related to thermogenesis in BAT and WAT were significantly increased, and the expression of Ampk, Serca1, Serca2 and Ucp3 which related to thermogenesis in skeletal muscle were significantly increased in SD mice. Taken together, these data demonstrated that 7-day SD enhanced the adaptive thermogenesis in mice by activating AMPK, including the upregulation of the AMPK - PGC1α - UCP1 pathway in BAT, and the AMPK - UCP3 and SLN - SERCA pathway in skeleton muscle. Our data provide the molecular evidence for SD-stimulated adaptive thermogenesis and energy metabolism in small mammals.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.90
自引率
0.00%
发文量
51
审稿时长
3.5 months
期刊介绍: The Journal of Comparative Physiology B publishes peer-reviewed original articles and reviews on the comparative physiology of invertebrate and vertebrate animals. Special emphasis is placed on integrative studies that elucidate mechanisms at the whole-animal, organ, tissue, cellular and/or molecular levels. Review papers report on the current state of knowledge in an area of comparative physiology, and directions in which future research is needed.
期刊最新文献
Oxidative stress across multiple tissues in house sparrows (Passer domesticus) acclimated to warm, stable cold, and unpredictable cold thermal treatments. Metabolic rate and saliva cortisol concentrations in socially housed adolescent guinea pigs. Metabolic effects of physical exercise on zebrafish (Danio rerio) fed a high-fat diet. Effects of in ovo supplementation of selenium (Se) and zinc (zn) on hatchability and production performance of broiler chickens. Microbial urea-nitrogen recycling in arctic ground squirrels: the effect of ambient temperature of hibernation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1