{"title":"一种基于苯并噻二唑支架的具有大斯托克斯偏移的荧光探针，用于选择性检测溶酶体中的 Hg2+ 及其在生物成像中的应用。","authors":"Lin Wang, Ning Zhang, Yuan He, Jian-Yong Wang","doi":"10.1002/asia.202401067","DOIUrl":null,"url":null,"abstract":"<p><p>Mercury is highly toxic, and appropriate amounts of the mercury-selenium complex can protect plasma. However, when excessive mercury (II) ions (Hg<sup>2+</sup>) are exposed to human skin or ingested directly, it can lead to irreversible accumulation in the body. Therefore, detecting the presence of Hg<sup>2+</sup> in cells is important. A novel fluorescent probe BTD-Hg-Lyso was designed and constructed based on the intramolecular charge transfer mechanism. Thiotaldehyde could specifically recognize Hg<sup>2+</sup> so that the probe could produce a fluorescence enhancement effect. In addition, the fluorescent probe BTD-Hg-Lyso exhibited the advantages of large Stokes shift (210 nm) and good selectivity. More importantly, the probe BTD-Hg-Lyso could be used for the determination of Hg<sup>2+</sup> in cellular lysosomes. BTD-Hg-Lyso was able to image Hg<sup>2+</sup> in HeLa cells, zebrafish, and tobacco seedlings (rhizome and stem) successfully.</p>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":" ","pages":"e202401067"},"PeriodicalIF":3.5000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Fluorescent Probe with Large Stokes Shift Based on Benzothiadiazole Scaffolds for Selective Detection of Hg<sup>2+</sup> in Lysosomes and Its Application in Biological Imaging.\",\"authors\":\"Lin Wang, Ning Zhang, Yuan He, Jian-Yong Wang\",\"doi\":\"10.1002/asia.202401067\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mercury is highly toxic, and appropriate amounts of the mercury-selenium complex can protect plasma. However, when excessive mercury (II) ions (Hg<sup>2+</sup>) are exposed to human skin or ingested directly, it can lead to irreversible accumulation in the body. Therefore, detecting the presence of Hg<sup>2+</sup> in cells is important. A novel fluorescent probe BTD-Hg-Lyso was designed and constructed based on the intramolecular charge transfer mechanism. Thiotaldehyde could specifically recognize Hg<sup>2+</sup> so that the probe could produce a fluorescence enhancement effect. In addition, the fluorescent probe BTD-Hg-Lyso exhibited the advantages of large Stokes shift (210 nm) and good selectivity. More importantly, the probe BTD-Hg-Lyso could be used for the determination of Hg<sup>2+</sup> in cellular lysosomes. BTD-Hg-Lyso was able to image Hg<sup>2+</sup> in HeLa cells, zebrafish, and tobacco seedlings (rhizome and stem) successfully.</p>\",\"PeriodicalId\":145,\"journal\":{\"name\":\"Chemistry - An Asian Journal\",\"volume\":\" \",\"pages\":\"e202401067\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemistry - An Asian Journal\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://doi.org/10.1002/asia.202401067\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry - An Asian Journal","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1002/asia.202401067","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
A Fluorescent Probe with Large Stokes Shift Based on Benzothiadiazole Scaffolds for Selective Detection of Hg2+ in Lysosomes and Its Application in Biological Imaging.
Mercury is highly toxic, and appropriate amounts of the mercury-selenium complex can protect plasma. However, when excessive mercury (II) ions (Hg2+) are exposed to human skin or ingested directly, it can lead to irreversible accumulation in the body. Therefore, detecting the presence of Hg2+ in cells is important. A novel fluorescent probe BTD-Hg-Lyso was designed and constructed based on the intramolecular charge transfer mechanism. Thiotaldehyde could specifically recognize Hg2+ so that the probe could produce a fluorescence enhancement effect. In addition, the fluorescent probe BTD-Hg-Lyso exhibited the advantages of large Stokes shift (210 nm) and good selectivity. More importantly, the probe BTD-Hg-Lyso could be used for the determination of Hg2+ in cellular lysosomes. BTD-Hg-Lyso was able to image Hg2+ in HeLa cells, zebrafish, and tobacco seedlings (rhizome and stem) successfully.
期刊介绍：
Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics.
Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews.
A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal.
Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).
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