用于促进跟腱愈合的富含 FGF-2 的纳米纤维支架:一项对比实验研究。

IF 1.6 4区 医学 Q2 SURGERY Frontiers in Surgery Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI:10.3389/fsurg.2024.1424734
Necmettin Turgut, Funda Cengiz Çallıoğlu, Aytül Bayraktar, Mehtap Savran, Halil Aşcı, Kanat Gülle, Meriç Ünal
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引用次数: 0

摘要

简介跟腱断裂是一种常见的致残性损伤,严重影响活动能力和生活质量。我们需要有效的治疗方案,以促进更快、更彻底的愈合。成纤维细胞生长因子-2(FGF-2)已显示出增强肌腱修复的潜力。本研究旨在调查 FGF-2 在跟腱断裂大鼠模型中促进肌腱愈合的功效,从而深入了解其作为治疗方案的潜力:48只跟腱完全断裂的大鼠后腿被分为四个等量组:假体(S)组(仅肌腱修复)、聚合物(P)组(带支架包裹的肌腱修复)、FGF-2(PF)组(涂有实验室生产的FGF-2的支架)和商业FGF-2(CF)组(涂有商业来源的FGF-2的支架)。手术后两周和四周的组织学分析根据核形态、血管、纤维组织、炎症和脂肪生成情况对愈合情况进行评估:第二周结束时,S 组和 P 组各有一只大鼠未观察到宏观愈合。第四周结束时,所有组均观察到大面积愈合。S 组和 P 组同样表现出严重的纤维重组、病理性脂肪生成和持续炎症,尤其是在第四周。相比之下,CF 组的肌腱愈合有所改善,血管和细胞外基质增加,炎症细胞浸润减少,纤维组织更好。CF组没有病理性脂肪生成,尤其是在第四周。PF组在第二周时有类似的改善,但到第四周时病情复发,炎症和脂肪生成增加:结论:在大鼠跟腱断裂模型中,FGF-2涂层支架通过改善纤维组织、增加血管性、减少炎症和病理性脂肪生成,明显促进了肌腱愈合。这些研究结果表明,FGF-2 可能是加速肌腱修复的一种有前途的治疗选择。肌腱修复的未来前景将集中在利用创新支架加强 FGF-2 的输送,为更有效的疗法和改善患者预后铺平道路。
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FGF-2 enriched nanofiber scaffold for advancing achilles tendon healing: a comparative experimental investigation.

Introduction: Achilles tendon rupture is a common and debilitating injury that significantly impacts mobility and quality of life. Effective treatment options that promote faster and more complete healing are needed. Fibroblast growth factor-2 (FGF-2) has shown potential in enhancing tendon repair. This study aims to investigate the efficacy of FGF-2 in promoting tendon healing in a rat model of Achilles tendon rupture, providing insights into its potential as a therapeutic option.

Materials and methods: Forty-eight rat hind legs with complete Achilles tendon ruptures were divided into four equal groups: the Sham (S) group (tendon repair only), the Polymer (P) group (tendon repair with scaffold wrapping), the Produced FGF-2 (PF) group (scaffold coated with lab-produced FGF-2), and the Commercial FGF-2 (CF) group (scaffold coated with commercially sourced FGF-2). Histological analyses at two and four weeks post-surgery evaluated healing based on nuclear morphology, vascularity, fibril organization, inflammation, and adipogenesis.

Results: At the end of the second week, no macroscopic healing was observed in one rat each from the S and P groups. By the end of the fourth week, macroscopic healing was observed in all groups. The S and P groups exhibited similarly severe fibril disorganization, pathological adipogenesis, and sustained inflammation, particularly at the fourth week. In contrast, the CF group demonstrated improved tendon healing with increased vascularity and extracellular matrix, lower inflammatory cell infiltration, and better fibril organization. Pathological adipogenesis was absent in the CF group, especially at the fourth week. The PF group showed comparable improvements at the second week but experienced a relapse by the 4th week, with increased inflammation and adipogenesis.

Conclusion: FGF-2 coated scaffolds significantly enhanced tendon healing in a rat Achilles tendon rupture model by improving fibril organization, increasing vascularity, and reducing inflammation and pathological adipogenesis. These findings suggest that FGF-2 could be a promising therapeutic option for accelerating tendon repair. Future perspectives on tendon repair will focus on enhancing FGF-2 delivery using innovative scaffolds, paving the way for more effective therapies and improved patient outcomes.

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来源期刊
Frontiers in Surgery
Frontiers in Surgery Medicine-Surgery
CiteScore
1.90
自引率
11.10%
发文量
1872
审稿时长
12 weeks
期刊介绍: Evidence of surgical interventions go back to prehistoric times. Since then, the field of surgery has developed into a complex array of specialties and procedures, particularly with the advent of microsurgery, lasers and minimally invasive techniques. The advanced skills now required from surgeons has led to ever increasing specialization, though these still share important fundamental principles. Frontiers in Surgery is the umbrella journal representing the publication interests of all surgical specialties. It is divided into several “Specialty Sections” listed below. All these sections have their own Specialty Chief Editor, Editorial Board and homepage, but all articles carry the citation Frontiers in Surgery. Frontiers in Surgery calls upon medical professionals and scientists from all surgical specialties to publish their experimental and clinical studies in this journal. By assembling all surgical specialties, which nonetheless retain their independence, under the common umbrella of Frontiers in Surgery, a powerful publication venue is created. Since there is often overlap and common ground between the different surgical specialties, assembly of all surgical disciplines into a single journal will foster a collaborative dialogue amongst the surgical community. This means that publications, which are also of interest to other surgical specialties, will reach a wider audience and have greater impact. The aim of this multidisciplinary journal is to create a discussion and knowledge platform of advances and research findings in surgical practice today to continuously improve clinical management of patients and foster innovation in this field.
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