从急性感染到持续感染:揭示阿戈纳沙门氏菌的系统发育变异。

IF 5.5 1区 医学 Q1 MICROBIOLOGY PLoS Pathogens Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.1371/journal.ppat.1012679
Emma V Waters, Winnie W Y Lee, Amina Ismail Ahmed, Marie-Anne Chattaway, Gemma C Langridge
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引用次数: 0

摘要

越来越多的人认识到,肠炎沙门氏菌血清阿戈纳菌(S. Agona)是导致肠胃炎的主要原因。阿戈纳沙门氏菌具有很强的生物膜形成能力,可进行基因组重排,进入可存活但不可培养的状态,同时保持新陈代谢活跃。伤寒杆菌(伤寒的病原体)在人类感染期间也采用了类似的策略,这被认为有助于从急性感染过渡到慢性携带。在此,我们报告了阿戈纳氏菌在人体内的存活能力,并研究了可能导致慢性携带的因素。我们对来自英国感染(2004-2020 年)和相关携带的 2233 株阿戈纳氏菌分离物进行了回顾,其中 1155 株有短线程测序数据。从个别患者的急性和持续感染的不同阶段选取了 207 个分离物子集。该子集进行了长线程测序和基因组结构(GS)分析,以及表型检测,包括碳源利用和生物膜形成。研究了基因型和表型之间的关联,以比较急性感染和发展为慢性感染的情况。GS 分析表明,195 个分离物中有 GS1.0 的保守排列,12 个分离物中有 8 个额外的 GS。这些重新排列的分离株通常与早期、恢复期(3 周至 3 个月)的携带有关。我们还发现,在这一感染时期,SNP 变异有所增加。我们认为,基因组规模和 SNP 变异的增加反映了急性 S. Agona 感染后的种群扩张,可能反映了一种免疫逃避机制,这种机制使持续感染得以确立。
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From acute to persistent infection: revealing phylogenomic variations in Salmonella Agona.

Salmonella enterica serovar Agona (S. Agona) has been increasingly recognised as a prominent cause of gastroenteritis. This serovar is a strong biofilm former that can undergo genome rearrangement and enter a viable but non-culturable state whilst remaining metabolically active. Similar strategies are employed by S. Typhi, the cause of typhoid fever, during human infection, which are believed to assist with the transition from acute infection to chronic carriage. Here we report S. Agona's ability to persist in people and examine factors that might be contributing to chronic carriage. A review of 2233 S. Agona isolates from UK infections (2004-2020) and associated carriage was undertaken, in which 1155 had short-read sequencing data available. A subset of 207 isolates was selected from different stages of acute and persistent infections within individual patients. The subset underwent long-read sequencing and genome structure (GS) analysis, as well as phenotyping assays including carbon source utilisation and biofilm formation. Associations between genotypes and phenotypes were investigated to compare acute infections to those which progress to chronic. GS analysis revealed the conserved arrangement GS1.0 in 195 isolates, and 8 additional GSs in 12 isolates. These rearranged isolates were typically associated with early, convalescent carriage (3 weeks- 3 months). We also identified an increase in SNP variation during this period of infection. We believe this increase in genome-scale and SNP variation reflects a population expansion after acute S. Agona infection, potentially reflecting an immune evasion mechanism which enables persistent infection to become established.

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PLoS Pathogens
PLoS Pathogens MICROBIOLOGY-PARASITOLOGY
自引率
3.00%
发文量
598
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
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