新诊断的急性髓细胞白血病（AML）中 CCL-3、FGF2、TPO 和 CTGF 的表达

IF 1 Q4 GENETICS & HEREDITY Gene Reports Pub Date : 2024-10-28 DOI:10.1016/j.genrep.2024.102068

Razieh Farkhondeh , Seyedeh Zahra Hasanpour , Mohsen Hamidpour , Mehdi Allah Bakhshian , Seyedeh Ommolbanin Ghasemian , Majid Gholami-Ahangaran

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引用次数: 0

摘要

材料与方法本研究调查了 60 名新诊断的急性髓细胞性白血病（AML）患者和 60 名健康志愿者的成纤维细胞生长因子 2（FGF2）、趋化因子（CC motif）配体 3（CCL-3）、血小板生成素（TPO）和结缔组织生长因子（CTGF）的表达情况。收集患者和健康人的骨髓和外周血样本，并使用 qRT-PCR 评估基因的表达。结果表明，与对照组相比，AML 患者的 CCL-3 和 FGF2 表达上调，而 TPO 表达下调。我们也没有发现 CTGF 的表达在患者和健康人之间有任何差异。此外，我们还发现基因表达与急性髓细胞性白血病患者的年龄和性别没有关联。只有 CTGF 的表达与急性髓细胞性白血病患者的囊泡百分比呈负相关。结论本研究提出，生长因子和细胞因子的表达可作为预后因素。然而，要更好地了解这些因子的确切作用，还需要在更大的统计人群中开展进一步的研究。

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The expression of CCL-3, FGF2, TPO and CTGF in newly diagnosed acute myeloblastic leukemia (AML)

Introduction

The interaction between Bone marrow (BM) microenvironment and acute myeloblastic leukemia (AML) cells is an essential event in the development of leukemia.

Material and methods

In this study investigated the expression of fibroblastic growth factor 2 (FGF2), chemokine (CC motif) ligand 3 (CCL-3), thrombopoietin (TPO) and connective tissue growth factor (CTGF) in 60 newly diagnosed AML patients and 60 healthy volunteers. BM and peripheral blood samples were collected from patients and healthy individuals and the expression of genes was assessed using qRT-PCR. The obtained data were analyzed using t-test, one-way ANOVA and Pearson correlation coefficient.

Results

The results showed that while the expression of CCL-3 and FGF2 was upregulated, the expression of TPO was downregulated in AML patients as compared with the control group. We also failed to find any difference in the expression of CTGF between patients and healthy individuals. Moreover, we found that there was no association between gene expression and the age and gender of AML patients. Only the expression of CTGF had a negative correlation with the percentage of blasts in AML patients. A positive significant correlation between FGF2 and CCL-3, FGF2 and TPO, FGF2 and CTGF as well as CCL-3 and TPO were detected.

Conclusions

This study proposed that the expression of growth factors and cytokines could be used as a prognostic factor. However, to gain better insight into the precise role of these factors, further studies at larger statistical population are required.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.